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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagcacttactttcattcatctataatgactgcgtgcagtttatttgaacagtcttcacttcctgtcttattattttctttaacctttcgtgcctggatctatgtacttcattcttgtggaatctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT

Basic information

species Zea mays
transcript GRMZM2G160430_T04
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G160430_T04 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os05g49260.1 (Oryza sativa), 3'ss of exon 8
-----------gtaagcacttactttcattcatctataatgactgcgtgcagtttatttgaacagtcttcacttcctgtcttatta---ttttctttaacctttcgtgcctggatctatgtactt-cattcttgtggaa--------------------tctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT
             | |||   | ||| |||  |||  | | ||||  | ||  |||||||     ||      |    ||||| ||   |||||  ||||    |      |||  ||||||||| |||| || |||||                       ||||||||| ||| |||||||| |||||||||||    ||| |  | |||  ||||||||||||||||||||||||||| ||  || | 
gtgaatgatataaaggcaagcaattttatttttcttgattaactgactacaagttatttgcggtttcgcgtgatttcatcttagtaagattttcactaacacaacac-ttgggagatatgtactttcatttttctggaagactaatatggttcaaatccctgttatgcagAGAGCAGTAGCATCGGCAAACAAAAGAACCACAGTCCGTTTTAAAGAGTTGAACCAAAAGAAATACCGCACTCCCTTACTG

upper sequence: GRMZM2G160430_T04 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os05g49270.1 (Oryza sativa), 3'ss of exon 8
gtaagcacttactttcattcatctataatgactgcgtgcagtttatttgaacagtcttcacttcctgtcttattattttctttaacctttcgtgcctggat-ctatgtacttc-----attcttgtggaatctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT
                                                     ||   |||||  | |   |||     |  | |  | | |  ||   || | |  | ||||      |    |    ||||||||||||| ||| ||||| ||||||||||||||    || ||  | |||| ||| || ||||| |||||||||||||| || ||||| 
-----------------------------------------------------gtaagcacttgttataacattgagcacactgatatat-gcaccacaataccacatgcttctggaagtaacagcatcatctttatgcagAGAGCAGTAGCGTCAGCAAACAAAAGGACAACGATCCGTTTTCAAGAATTAAACCAGAAGAAATACCGCACACCCCTAATA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32832539|gb|CD972217.1|CD972217
EST:     TTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAG                         ATAGCTGTAGCATCAGCAAACAAAAGTGGT
genomic: TTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAGgtaagcactt ... ctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGT
EST: gi|32929449|gb|CF034261.1|CF034261
EST:     TTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAG                         ATAGCTGTAGCATCAGCAAACAAAAGTGGT
genomic: TTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAGgtaagcactt ... ctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGT
EST: gi|32834892|gb|CD974570.1|CD974570
EST:     TTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAG                         ATAGCTGTAGCATCAGCAAACAAAAGTGGT
genomic: TTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAGgtaagcactt ... ctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 38 (downstream exon)
Score: 10

GCGAGGATGAATATGATGGATGATTGCGAGACTTCTCTACAAGTTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAGgtaagcactt ... ctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 152

GCGAGGATGAATATGATGGATGATTGCGAGACTTCTCTACAAGTTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAGgtaagcactt ... ctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 108

GCGAGGATGAATATGATGGATGATTGCGAGACTTCTCTACAAGTTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAGgtaagcactt ... ctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT


Block sizes: 46 (upstream exon), 47 (downstream exon)
Score: 68

GCGAGGATGAATATGATGGATGATTGCGAGACTTCTCTACAAGTTCTGAGGGGATTTGATGCTGACATCACTGAAGAAGTGAATGATATAAAGgtaagcactt ... ctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttaacctttcgtgcctggatctatgtacttcattcttgtggaatctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT
 ctttaac  putative branch site (score: 68)
                                            tctttat  putative PPT
                                          aatctttat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagcacttactttcattcatctataatgactgcgtgcagtttatttgaacagtcttcacttcctgtcttattattttctttaacctttcgtgcctggatctatgtacttcattcttgtggaatctttatgcagATAGCTGTAGCATCAGCAAACAAAAGTGGTACAATAAGCTTTCGAGAGTTGAACCAAAAGAAATACCGCACGCCTCTAATT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAA