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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtgatcactccatgtacgtgggtcatcagtggactaccccagcttatgtctgcctacaatctgattgttcttttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG

Basic information

species Zea mays
transcript GRMZM2G155836_T01
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G155836_T01 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os05g04340.2 (Oryza sativa), 3'ss of exon 8
gtaagtgatcactccatgtacgtgggtcatcagtggactaccccagcttat-------gtctgcctacaatctg--attgttcttttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG
||||| ||||||   || ||| || | | |   ||||| | || |||| ||       |||||||||   | |    || || | |||||| ||||||| |||||||| || || || |||||||||||||||||||||||||||
gtaagagatcacattatttacatgaggcttt--tggaccatcc-agctcattgcaactgtctgcctaagctttatcctttttttgttttaaaagCCTCTATTCCCTGGCGACAGTGGCGTTGATCAGCTTGTTGAAATCATCAAG

upper sequence: GRMZM2G155836_T01 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA10G01280.1 (Glycine max), 3'ss of exon 8
gtaagtgatcactccatgtacgtgggtcatcagtggactaccccagcttatgtctgcctacaatctgattgttcttttttaac------agCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG
     |  |   |  | ||| ||  |       |  | |     |  | |  ||||   |  | || ||  |   ||| || |      ||||||| |||||||| || || || || ||||||||||||||||| ||||||
--gtatcttgcttttaagtatgtttgattctgctatatt--tagaattcacatctgtgcaggaactaataatgaatttctatcgttgttagCCTCTATTCCCTGGTGAGAGTGGAGTAGATCAGCTTGTTGAAATTATCAAG

upper sequence: GRMZM2G155836_T01 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA02G01220.1 (Glycine max), 3'ss of exon 8
gtaagtgatcactccatgtacgtgggtcatcagtggactaccccagcttatgtctgcctacaatctgattgttcttttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG
|||  |      |   | |   ||  ||  |  |  |  |  |     | ||   |  |  |   ||| | |   || ||   ||||||| |||||||| || || || || ||||||||||||||||| ||||||
gtatcttgcttttaagtatgtttgattctgctatttagaattcacatctgtgcaagaattaataatgaatttctgttgttgttagCCTCTATTCCCTGGTGAGAGTGGAGTAGATCAGCTTGTTGAAATTATCAAG

upper sequence: GRMZM2G155836_T01 (Zea mays), 3'ss of exon 8
lower sequence: PP1S52_55V6.1 (Physcomitrella patens), 3'ss of exon 7
----------------------------------------------------------------------------------------gtaagtgatcactccatgtacgtgggtcatcagtggactaccccagcttatgtctgcctacaa----tctgattgttcttttttaa--------cagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG
                                                                                        ||   |  | |    ||||| |||| |  | |   ||  |     | ||  ||  |   | |     ||||  ||  |  |||  |        |||||| ||||||||||||||||||| ||||||||  | || || |||||||||
gtacttatgtagttcctggtagatggtgaattcatggagattctattactcctgagtactatggttcggaatggagatagatcattgtgttgattgtaatgagatgtaagtggttggtgaagtgatcattgttggtttagttagatgatatgaattctgtctggacaatttcgatgttggtgcagCCTTTGTTCCCTGGAGAAAGCGGAGTTGATCAATTGGTGGAGATCATCAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|6194846|gb|AW146950.1|AW146950
EST:     TTGATGTTTGGTCTGCTGGCTGTGTGCTCGCTGAGCTGCTTCTAGGACAG                         CCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG
genomic: TTGATGTTTGGTCTGCTGGCTGTGTGCTCGCTGAGCTGCTTCTAGGACAGgtaagtgatc ... tttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG




















RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 98

CTACAGAGCTCCAGAGCTCATATTTGGTGCTACTGAATACACAACAGCCATTGATGTTTGGTCTGCTGGCTGTGTGCTCGCTGAGCTGCTTCTAGGACAGgtaagtgatc ... tttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 142

CTACAGAGCTCCAGAGCTCATATTTGGTGCTACTGAATACACAACAGCCATTGATGTTTGGTCTGCTGGCTGTGTGCTCGCTGAGCTGCTTCTAGGACAGgtaagtgatc ... tttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 121

CTACAGAGCTCCAGAGCTCATATTTGGTGCTACTGAATACACAACAGCCATTGATGTTTGGTCTGCTGGCTGTGTGCTCGCTGAGCTGCTTCTAGGACAGgtaagtgatc ... tttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 237

CTACAGAGCTCCAGAGCTCATATTTGGTGCTACTGAATACACAACAGCCATTGATGTTTGGTCTGCTGGCTGTGTGCTCGCTGAGCTGCTTCTAGGACAGgtaagtgatc ... tttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cagtggactaccccagcttatgtctgcctacaatctgattgttcttttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG
                                atctgat  putative branch site (score: 237)
                                      ttgttctttttt  CT-rich tract
                                     attgttcttttttaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtgatcactccatgtacgtgggtcatcagtggactaccccagcttatgtctgcctacaatctgattgttcttttttaacagCCTCTGTTCCCTGGAGAAAGCGGTGTTGATCAGCTTGTTGAAATCATCAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TCAGCT