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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtcagcaaatctgaaacctgttctttttagttttgactcctggtttttctcctcttcttaatgctgtttgtgtaccggattgtagGGTCCTCCCAGACACAATGTTTGAGAAACTGAAATGGCTGTACATCACTGTTAATATTGGTGAAATAAAACAGATACCGAATC

Basic information

species Zea mays
transcript GRMZM2G114131_T01
intron # 8
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148944967|gb|EC890137.2|EC890137
EST:     ACATGAACCTGAAGACATTTAAATCGGGTTCAACACTACCTTACTCACTG                         GGTCCTCCCAGACACAATGTTTGAGAAACTGAAATGGCTGTACATCACTGT
genomic: ACATGAACCTGAAGACATCTAAATCGGGTTCAACACTACCTTACTCACTGgtcagcaaat ... cggattgtagGGTCCTCCCAGACACAATGTTTGAGAAACTGAAATGGCTGTACATCACTGT
EST: gi|60401013|gb|DN233820.1|DN233820
EST:     AGATGAACCTAAACACATCTAAATCGGGTTCAACACTACCTTACTCACTG                         GGTC-TCCCAGACACAATGTTTGAGAAACTGAAATGGTTGTACATCACTGT
genomic: ACATGAACCTGAAGACATCTAAATCGGGTTCAACACTACCTTACTCACTGgtcagcaaat ... cggattgtagGGTCCTCCCAGACACAATGTTTGAGAAACTGAAATGGCTGTACATCACTGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 2 RNA-Seq libraries.


Block sizes: 25 (upstream exon), 25 (downstream exon)
Score: 1

AGTTACAAGAATGCTACCAAGCTGTTTTCTTATCCTGGCTCCAAAGTTCAACATGAACCTGAAGACATCTAAATCGGGTTCAACACTACCTTACTCACTGgtcagcaaat ... cggattgtagGGTCCTCCCAGACACAATGTTTGAGAAACTGAAATGGCTGTACATCACTGTTAATATTGGTGAAATAAAACAGATACCGAATC


Block sizes: 12 (upstream exon), 38 (downstream exon)
Score: 3

AGTTACAAGAATGCTACCAAGCTGTTTTCTTATCCTGGCTCCAAAGTTCAACATGAACCTGAAGACATCTAAATCGGGTTCAACACTACCTTACTCACTGgtcagcaaat ... cggattgtagGGTCCTCCCAGACACAATGTTTGAGAAACTGAAATGGCTGTACATCACTGTTAATATTGGTGAAATAAAACAGATACCGAATC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttttgactcctggtttttctcctcttcttaatgctgtttgtgtaccggattgtagGGTCCTCCCAGACACAATGTTTGAGAAACTGAAATGGCTGTACATCACTGTTAATATTGGTGAAATAAAACAGATACCGAATC
                         tcttaat  putative branch site (score: 3)
                        ttcttaat  TA-rich tract