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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttttttgtttctgtatgtggctgggaaaaatgtttttatgcattctgatatcatatcccatttggcactgatgacttgcttaccttttttttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGCAACTTCTTTGAGAAATAAGGAGATGATCGGTAAATCAGACCCTTATGTG

Basic information

species Zea mays
transcript GRMZM2G092129_T02
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G092129_T02 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os07g22640.3 (Oryza sativa), 3'ss of exon 2
ttttttgtttctgtatgtggctgggaaaaatgttttta------tgcattctgatatcatatcccatttggcactgatgacttgcttaccttttttttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGCAACTTCTTTGAGAAATAAGGAGATGATCGGTAAATCAGACCCTTATGTG
 ||||||||| | | |    |||  ||  || ||| ||      ||||| |   |  ||| |    |     | |  | | |||     || |  || |||   |||||  |||||||||||||||||||||||||| |||||||||| |||||||||||||| ||||  ||||||||| |||| ||||||||||| || ||||||
-tttttgtttatttgtt---ctgaaaagtatctttataatgaattgcatcccattggcatgtaatttcaaaaatt--ttatttgtcctgctctacttcatctgcagTGAGCTGGAGCTTAAACCTCAGGGAAGACTTACTGTTACTGTAGTAAAAGCAACTTCATTGAAGAATAAGGAGTTGATTGGTAAATCAGATCCATATGTG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211220819|gb|FL457378.1|FL457378
EST:     TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAG                         TGACTTGGAGCTTAAACCTCAGGGAAGACTTTYTGTTACTGTGGTAAAAGC
genomic: TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
EST: gi|211335355|gb|FL444766.1|FL444766
EST:     TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAG                         TGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
genomic: TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
EST: gi|67025946|gb|CO454695.1|CO454695
EST:     TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCTATGTTGATA-GAG                         TGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
genomic: TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
EST: gi|67011685|gb|CO440434.1|CO440434
EST:     CACACA-GCTTGTTGTTAAACTTGGTGTCAATGTTGCTACGAG                         TGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
genomic: CACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
EST: gi|211220820|gb|FL457379.1|FL457379
EST:     TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAG                         TGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
genomic: TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
EST: gi|111145863|gb|EE258112.1|EE258112
EST:     TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAG                         TGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC
genomic: TTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 235

GACACTGTCGATTCAATTGTTTCTGATATGCTCTTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGCAACTTCTTTGAGAAATAAGGAGATGATCGGTAAATCAGACCCTTATGTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 297

GACACTGTCGATTCAATTGTTTCTGATATGCTCTTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGCAACTTCTTTGAGAAATAAGGAGATGATCGGTAAATCAGACCCTTATGTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 299

GACACTGTCGATTCAATTGTTTCTGATATGCTCTTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGCAACTTCTTTGAGAAATAAGGAGATGATCGGTAAATCAGACCCTTATGTG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 214

GACACTGTCGATTCAATTGTTTCTGATATGCTCTTGTGGCCACACAGGCATGTTGTTAAACTTGGTGTCAATGTTGATACGAGgtgggtttcc ... ttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGCAACTTCTTTGAGAAATAAGGAGATGATCGGTAAATCAGACCCTTATGTG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgatatcatatcccatttggcactgatgacttgcttaccttttttttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGCAACTTCTTTGAGAAATAAGGAGATGATCGGTAAATCAGACCCTTATGTG
                               tgcttac  putative branch site (score: 214)
                             cttgcttacctttttt  CT-rich tract
                                  ttaccttttttttat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttttttgtttctgtatgtggctgggaaaaatgtttttatgcattctgatatcatatcccatttggcactgatgacttgcttaccttttttttatccctagTGACTTGGAGCTTAAACCTCAGGGAAGACTTTCTGTTACTGTGGTAAAAGCAACTTCTTTGAGAAATAAGGAGATGATCGGTAAATCAGACCCTTATGTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGATG