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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...agatgatggactagctcattcctcaaaccaaacaccctattactctcttttttggttcacttcttcattttaaactttttatgacctatgcaaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG

Basic information

species Zea mays
transcript GRMZM2G073054_T01
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G073054_T02 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os04g33460.1 (Oryza sativa), 3'ss of exon 8
---------------------------------------------------------------agatgatggactagctcattcctcaaaccaa-acaccctattactctcttttttggttcac-----ttcttcattttaaacttttt---------------atgacctatgcaaaa-tgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG
                                                                 ||| | |  |  || |  ||| | |  |        || |||| | ||| || ||| |      |  | |||||||| |||| |                |    ||| |  || ||||||| |||||||||||| |||| ||||||||||| || || || || ||||||||||||||||||||| |||||||||||||||||
gtatgtttctaccttacagtctttccacgaaatatttttcccttgcattttttttaaaaaaatctatgctcgtgtcactaaaaccttatagaactgtgtaatactactttttttctttgttgatcaggatattccattttaacctttattttccatctcctaatgttgtttatacgcaactgcagGAAAAATATGTATTCCAACATCCTCAACCTAAACGACCAAATTCGCTGCGGATATATGAATCACATATTGGAATGAGTAGCCCG

upper sequence: GRMZM2G073054_T02 (Zea mays), 3'ss of exon 8
lower sequence: AT2G36390.1 (Arabidopsis thaliana), 3'ss of exon 8
-agatgatggactagctcattcctcaaaccaaacaccctattactctcttttttggttcacttcttcattttaaactttttatgacctatgcaaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG
 |  || |   |    | ||   |      |       |  ||      | | |   |   | | ||  |  |  | |   | |  ||        | ||||| |||||||  |||||||| ||||  || |||   ||||  || ||||| |||||||||||||||||||||||||||||  ||
taactggtatccagcattatctatgggtatatgtggtttcatatatatatatatatatatatccctcgctgaaggcctaaaaagtactccattgt-ttcagGATAAATATGCGTTCAAACATCCTCGTCCAAAGAAACCCACATCGCTGCGTATATATGAATCACATGTTGGAATGAGTAGTACG

upper sequence: GRMZM2G073054_T02 (Zea mays), 3'ss of exon 8
lower sequence: AT5G03650.1 (Arabidopsis thaliana), 3'ss of exon 8
-------------------------------------------------------------------------------agatga-tggactagctcattcctcaaaccaaacaccctat-tactctcttttttggttcacttcttcattttaaactttttatgacct-------atgcaaaatgc------agGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG
                                                                               ||||   | ||  || |   ||    ||    |     ||| | ||  |  |||    | |   | |||   || ||||||  |  ||         | | ||||        ||||||| |||||||||||||| |||||||| ||| | || ||||| ||  |||| |||||| |||||||||| ||||||||| ||
gtaaggctctacagtcttttgaataattatcttgtggaaaacaattgtgccagtgctagtctgaaatttttgaagatttagatttctcgataagttttctcagtcaattccatttaatatatgcttacaatttaatataaaaacatcaacatatacttttgcttgcccttcaacggttccaaatttctttttagGAGAAGTATGTATTCAAACATCCTCAACCAAAGAGACCTAAGTCGCTAAGGATTTATGAAGCACATGTTGGCATGAGTAGCACG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|22472538|gb|BU037018.1|BU037018
EST:     CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAG                         GAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG
genomic: CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG
EST: gi|211263679|gb|FL032932.1|FL032932
EST:     CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAG                         GAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCAAAGTCACTGNGG
genomic: CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG
EST: gi|87155797|gb|DY400586.1|DY400586
EST:     CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAG                         GAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG
genomic: CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG
EST: gi|26558524|gb|CA830759.1|CA830759
EST:     CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAG                         GAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG
genomic: CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG
EST: gi|67022129|gb|CO450878.1|CO450878
EST:     CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAG                         GAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG
genomic: CTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 675

ATCTGGTGTTAAGGATTCCATTCCTGCCTGGATCAAGTTTTCTGTGCAGGCTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 193

ATCTGGTGTTAAGGATTCCATTCCTGCCTGGATCAAGTTTTCTGTGCAGGCTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG


Block sizes: 47 (upstream exon), 46 (downstream exon)
Score: 13

ATCTGGTGTTAAGGATTCCATTCCTGCCTGGATCAAGTTTTCTGTGCAGGCTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 247

ATCTGGTGTTAAGGATTCCATTCCTGCCTGGATCAAGTTTTCTGTGCAGGCTCCAGGTGAAATACCATACAACGGTATATATTATGACCCACCTGAAGAGgtatgctcaa ... caaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tcttttttggttcacttcttcattttaaactttttatgacctatgcaaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG
               ttcttcattttaaact  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agatgatggactagctcattcctcaaaccaaacaccctattactctcttttttggttcacttcttcattttaaactttttatgacctatgcaaaatgcagGAGAAATATGTATTCAAACACCCTCAACCTAAGCGGCCCAAGTCACTGCGGATATATGAATCACATGTTGGAATGAGTAGCCCG

-gatgatg
- - - - - - - - cattcct
- - - - - - - - - - - - - -ccaaaca