ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...tgtgtggattattatcagttcggttatgattatcttttctgacctttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG

Basic information

species	Zea mays
transcript	GRMZM2G014805_T01
intron #	8
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os12g30540.1 (Oryza sativa), 3'ss of exon 8

---tgtgtggattattatcagttcggttatgat-tatcttttctgacctttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG
   | |     |||  || | |     |||    ||  |    |||   | |||| |||    |  |  ||       | ||  ||||| | |  | |||||||||||||| ||| || || || |||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| |||
gattttccaagtta--atgaatggcaatatagcatacatgccatgattatattttcttacc-ttgatactgcattggcactt-gatattctgtaatcttttcagGATGCAAAGAAGGGAGTACTCTTCCTTGATTTCCCTCCTGTTTTGCAACTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTCAAG

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA14G04890.1 (Glycine max), 3'ss of exon 8

-tgtgtggattattatcagttcggttatgattatcttttctgacctttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG
 ||  |   |||   |   |   |||    | |  |    | | ||     | || ||| ||| |||   | |  |   | ||  | |||  | |||||||||||| | ||| ||||| || ||| ||||||||||||||||| | ||||||||||||||  | ||||||||||| |  ||||| ||||| ||||| |||
ctgaatcctttaagtttcttatagttgctgtcaa-tgcaataatctacacatttttttaatatagtttcacgtataaactgatgatttttcattttttcagGATGCTAAGAAGGGTGTCCTGTTCATTGATTTCCCTCCTGTTCTTCAGCTTCAACTGAAAAGATTTGAATATGACTTTATGCGGGATACTATGGTCAAG

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 8
lower sequence: GLYMA02G43930.1 (Glycine max), 3'ss of exon 8

-tgtgtggattattatcagttcggttatgattatcttttctgacctttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG
 ||  |   |||   |   |   ||||   | |   |     | || |   | || ||| ||| |||   | |  |   | ||  | |||  | |||||||||||| | ||| ||||| || ||| ||||||||||||||||| | ||||||||||||||  | ||||||||||| |  ||||| ||||| ||||| |||
ctgaatcttttaagcttcttatagttactgtcagaatgcaatatctatacat-tttttaatatagttccacgtataaactgatgatttttcattttttcagGATGCTAAGAAGGGTGTCCTGTTCATTGATTTCCCTCCTGTTCTTCAGCTTCAACTGAAAAGATTTGAATATGACTTTATGCGGGATACTATGGTCAAG

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 8
lower sequence: Vv06s0009g00960.t01 (Vitis vinifera), 3'ss of exon 8

--------tgtgtggattattatcagttcggttatgattatcttttctgacctttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG
        | | |    | ||| |  || | | ||||  | ||  |   |  |||   | |   |    |   ||||  || | |||  |||  || | |     |||||||| ||||| |||||  | ||| |||| |||||||||||  | || ||||| |||||||| |||||||||||||  ||||| || || ||||| |||
gggtttcatatttaccgtcttacccctttg-tcatgaaaacctcattctatgtttcgctttccatcaagtgactgtt--ttgaatttggtatgcttgtct-----cagGATGCTAGGAAGGGTGTCTTATTCATTGACTTCCCTCCTGTCCTTCAACTTCAGCTGAAGCGATTTGAATATGATTTTATGCGGGACACTATGGTAAAG

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 8
lower sequence: EFJ18164 (Selaginella moellendorffii), 3'ss of exon 6

tgtgtggattattatcagttcggttatgattatcttttctgacctttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG
                                                |||||| ||  | ||     ||   ||  ||  || |  || ||  ||| |||||||| | || || ||||| ||  | ||||| || |||||| | ||||||||||| ||||| |||||||||||||   ||||||| ||||||||||||
-----------------------------------------------gtttgtttttcgtgttccatgtcaagaaggtt--taatggttgtttcttttagGATGCACGCAAGGGCGTGCTCTTTGTGGATTTTCCCCCTGTTCTCCAGCTTCAACTCAAGCGGTTTGAATATGATTTTGTGCGAGACACAATGGTTAAG

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 8
lower sequence: EFJ14291 (Selaginella moellendorffii), 3'ss of exon 6

tgtgtggattattatcagttcggttatgattatcttttctgacctttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG
                                                |||||| ||  | ||     ||   ||  ||  || |  || ||| ||| |||||||| | || || ||||| ||  | ||||| || |||||| | ||||||||||| ||||| |||||||||||||   ||||||| ||||||||||||
-----------------------------------------------gtttgtttttcgtgttccatgtcaagaaggtt--taatggttgttgcttttagGATGCACGCAAGGGCGTGCTCTTTGTGGATTTTCCCCCTGTTCTCCAGCTTCAACTCAAGCGGTTTGAATATGATTTTGTGCGAGACACAATGGTTAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|5439328|gb|AI820249.1|AI820249

EST:     AGGGTGAT-ACAAGTACCATGCGGAGCGTTATGGCTTACAG                         GATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT
genomic: AGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAGgttcccacaa ... ttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT

EST: gi|5740126|gb|AI947816.1|AI947816

EST:     TACCATGCGGAGCGTTATGGCTTACAG                         GATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT
genomic: TACCATGCGGAGCGTTATGGCTTACAGgttcccacaa ... ttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT

EST: gi|101389686|gb|EB816777.1|EB816777

EST:     AGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAG                         GATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT
genomic: AGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAGgttcccacaa ... ttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT

EST: gi|76920464|gb|DV168186.1|DV168186

EST:     AGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAG                         GATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT
genomic: AGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAGgttcccacaa ... ttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT

EST: gi|78026582|gb|DV494969.1|DV494969

EST:     AGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAG                         GATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT
genomic: AGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAGgttcccacaa ... ttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT

EST: gi|5398807|gb|AI812151.1|AI812151

EST:     AGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAG                         GATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT
genomic: AGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAGgttcccacaa ... ttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 47 (downstream exon)
Score: 200

GAAAGGTTGTCGTGATGTTTATGCATCATTTGATAAGTATGTGGAAGTGGAGAGTCTAGAGGGTGATAACAAGTACCATGCGGAGCGTTATGGCTTACAGgttcccacaa ... ttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 208

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 199

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 155

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG

                              ttttaat  putative branch site (score: 155)
                                      ttattttttgttttc  CT-rich tract
       ttattactattgttgt  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgtgtggattattatcagttcggttatgattatcttttctgacctttttttgttattactattgttgttccttaattttaatattattttttgttttcagGATGCAAGGAAAGGTGTGCTTTTCCTTGATTTCCCTCCTGTTTTGCAGCTTCAACTGAAGCGTTTTGAATATGATTACATGCGAGATACAATGGTTAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGC
- - - - - - - - - - - - - - - - - ttttctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tgttgtt