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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gcaagaatagtgcttcttattacctatgattaatcataaatttatttctggcaattataattcatacatacatttttttaaaatgatacctttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGGTCTTTGGTTCCTTCAAAG

Basic information

species Zea mays
transcript AC209858.4_FGT002
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: AC209858.4_FGT002 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os03g05720.1 (Oryza sativa), 3'ss of exon 8
gcaagaatagtgcttcttattacctatgattaatcat-----aaatttatttctg-gcaattataattcatacatacatttttttaaaatgatacctttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGGTCTTTGGTTCCTTCAAAG
      || ||| |  |||  |  | | |  || ||      | ||| ||   ||   | | || ||||||   |  ||| | |    ||| |  |  || | ||| ||||||| ||||| |||||||| ||| ||||||||||||| |||||||||||| |||||||||  |||
------attgtgttcgttagcatatttaaaaaaccactaaacagattcatccttgtatattaatcattcatcatttgattatatatttatgttttccctgcaacagTCATTGCCATTCTCAGATGCACTTAAGATCATGTCTTACTTAAAGGAGTGGTCTATGGTTCCTTTGAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32833350|gb|CD973028.1|CD973028
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|32937153|gb|CF041972.1|CF041972
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|32932056|gb|CF036868.1|CF036868
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|32835694|gb|CD975372.1|CD975372
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|32835920|gb|CD975598.1|CD975598
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|32832909|gb|CD972587.1|CD972587
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCGCTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|211417128|gb|FL448753.1|FL448753
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|78084366|gb|DV512759.1|DV512759
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTTTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|211057077|gb|FL049064.1|FL049064
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|32833351|gb|CD973029.1|CD973029
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
EST: gi|78084367|gb|DV512760.1|DV512760
EST:     TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTG                         GCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG
genomic: TGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 88

ATTTCAACCTAATGTTATAATGCAAGGGCATTCACCTTCAGATTATGTTCTGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGGTCTTTGGTTCCTTCAAAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 31

ATTTCAACCTAATGTTATAATGCAAGGGCATTCACCTTCAGATTATGTTCTGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGGTCTTTGGTTCCTTCAAAG


Block sizes: 41 (upstream exon), 49 (downstream exon)
Score: 61

ATTTCAACCTAATGTTATAATGCAAGGGCATTCACCTTCAGATTATGTTCTGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGGTCTTTGGTTCCTTCAAAG


Block sizes: 41 (upstream exon), 47 (downstream exon)
Score: 40

ATTTCAACCTAATGTTATAATGCAAGGGCATTCACCTTCAGATTATGTTCTGAATGTAGTTTCAAGCATCCGTCCAAATGATCTGGAGCAGGCCCTCTTGgtatgtattt ... tttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGGTCTTTGGTTCCTTCAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttctggcaattataattcatacatacatttttttaaaatgatacctttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGGTCTTTGGTTCCTTCAAAG
                                         taccttt  CT-rich tract
       aattataattcataca  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gcaagaatagtgcttcttattacctatgattaatcataaatttatttctggcaattataattcatacatacatttttttaaaatgatacctttggaccagGCATTGCCGTTCTCGGATGCACTAAAGCTCATGTCTTACTTGAAGGAGTGGTCTTTGGTTCCTTCAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAG
- aagaatag
- - - - - tgcttct
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -acataca