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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gacatcagtgggtttggcattagaaataatcagattgtcatcctcttgcttgctgatatcaaattccctcgctaattttgttttcttctgtcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC

Basic information

species Zea mays
transcript GRMZM2G173424_T02
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G173424_T02 (Zea mays), 3'ss of exon 7
lower sequence: LOC_Os12g40510.1 (Oryza sativa), 3'ss of exon 7
-------gacatcagtgggtttggcattagaaataatcagattgtcatcctcttgcttgctgatatcaaattccctcgctaattttgttttcttctgtcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC
         | ||| | || ||||||| ||| ||  |||| ||   ||| | || |||  | ||   || ||||   |||||      | | || | | |||   ||||||||||||||||||||||||||||||||||||||||||| || ||||||||||||||||| ||  |||| ||||| ||||||||||||||||||||||
atgacatatcctcaataggcttggcataagatatggtcagttt---atcttgtttctttttaattgtaacttccaatgctaa----actattttttcttatttgtagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGATAAAGAAGAGTGGCGCAATCTGGTTCTTCGTCATCCCTCTTTGCTGAAGAACATGACTC

upper sequence: GRMZM2G173424_T02 (Zea mays), 3'ss of exon 7
lower sequence: GLYMA08G44580.1 (Glycine max), 3'ss of exon 7
-----------------------gacatcagtgggtt-tggcattagaaataa----tcagattgtcatcctcttgcttgctgatatcaaattccctcgctaattttgttttc----ttctgtcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC
                        ||||   |  ||| |   | |  | || |    | ||| | ||||  |||  |||  |||    || |  | | | || |||     |     ||  |   |    |||| || ||||| ||||| |||||||||||||| |  |||||||||||||||||| | || || ||  | || ||||| ||  |  ||||||||||||| |
gttttaccccattttctataacttacatttctaagttctaatagtgcacatgagtcttaagagtctcattttct--ctttttga---aaactcacatggatagtttcaacattaaattttggatttctttagACCTTCGAGGAAGCAGATACAAAGCATGATGGCAAGATTGACAAGGAAGAGTGGAGAAACCTTGTTTTGCGCCATCCGTCACTTTTGAAGAACATGACCC

upper sequence: GRMZM2G173424_T02 (Zea mays), 3'ss of exon 7
lower sequence: GLYMA18G08230.1 (Glycine max), 3'ss of exon 8
------------------------gacatcagtgggtttggcattagaaataatcagattgtcatcctcttgcttgctgatatcaaattccctcgc-taattttgttttc---ttctgtcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC-
                         ||||   |  |||    |||    | ||  |  ||   |  |||||  |  ||  |   ||| || |  |  || ||| |   |    || ||   ||   |||| || ||||| ||||| |||||||||||||| | ||||||||||||||||||| | || || ||  | || ||||| ||  |  ||||||||||||| | 
ttttacccctattcttctatcacttacatttctaagttcta-attgtgcacaagaatcttaagagtctctttttctctttttgaaaactcacatgaatagtttcgacattaaattttggatttcttagaCCTTCGAGGAAGCAGATACAAAGCATGATGGCAAAATTGACAAGGAAGAGTGGAGAAACCTTGTTTTGCGCCATCCGTCACTTTTGAAGAACATGACCCT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211119821|gb|FL462978.1|FL462978
EST:     AGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAG                         ACATTTGAGGAGGCAGACACAAAGCWTGATGGAAGAATTGACAAGGAAGAG
genomic: AGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAGgtctgttaaa ... tcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAG
EST: gi|88755366|gb|DY539507.1|DY539507
EST:     AGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAG                         ACATTTGAGGAGGCGGACACAAAGCATGATGGAAGAATTGACAAGGAAGAG
genomic: AGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAGgtctgttaaa ... tcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAG
EST: gi|74244689|gb|DT652603.1|DT652603
EST:     AGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAG                         ACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAG
genomic: AGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAGgtctgttaaa ... tcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAG
EST: gi|71307467|gb|DR789938.1|DR789938
EST:     AGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAG                         ACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAG
genomic: AGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAGgtctgttaaa ... tcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 319

GTTAAGCAGATGGTTGTTGCAACACTTGCCGAGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAGgtctgttaaa ... tcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 221

GTTAAGCAGATGGTTGTTGCAACACTTGCCGAGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAGgtctgttaaa ... tcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 262

GTTAAGCAGATGGTTGTTGCAACACTTGCCGAGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAGgtctgttaaa ... tcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 230

GTTAAGCAGATGGTTGTTGCAACACTTGCCGAGTCAGGAATGAATCTTTCGGATGAAATTGTAGAGAGTATAATTGACAAGgtctgttaaa ... tcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttgcttgctgatatcaaattccctcgctaattttgttttcttctgtcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC
                        cgctaat  putative branch site (score: 230)
                              ttttgttttcttctgt  CT-rich tract
                           taattttgttttctt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gacatcagtgggtttggcattagaaataatcagattgtcatcctcttgcttgctgatatcaaattccctcgctaattttgttttcttctgtcattacaagACATTTGAGGAGGCAGACACAAAGCATGATGGAAGAATTGACAAGGAAGAGTGGCGCAATCTAGTCATTCGGCATCCTTCTTTGCTGAAGAACATGACTC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAC
- - - - - - - - - - -agaaataa
- - - - - - - - - - - - - - - - - - - catcctc
- - - - - - - - - - - - - - - - - - - - - - - tgcttgc