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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgctaaccttttcatgtttgctttagtatttttctcgtaaatgtggacattctagagtactgctacatattattgatgcatgtttgttttgtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG

Basic information

species Zea mays
transcript GRMZM2G149321_T01
intron # 7
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211428993|gb|FL402814.1|FL402814
EST:     TAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAG                         GCACAAGGATCACTTGAACGAGATGATGAG
genomic: TAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAGgtgataacat ... gtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG
EST: gi|31364305|gb|CD448660.1|CD448660
EST:     TAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAG                         GCACAAGGATCACTTGAACGAGATGATGAG
genomic: TAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAGgtgataacat ... gtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG
EST: gi|211266310|gb|FL390568.1|FL390568
EST:     TAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAG                         GCACAAGGATCACTTGAACGAGATGATGAG
genomic: TAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAGgtgataacat ... gtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 37 (downstream exon)
Score: 164

TGATGACTTCCGGGACAGGGATTACGACGTGGCAGCTCTTGCTAATAACTTAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAGgtgataacat ... gtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 145

TGATGACTTCCGGGACAGGGATTACGACGTGGCAGCTCTTGCTAATAACTTAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAGgtgataacat ... gtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 149

TGATGACTTCCGGGACAGGGATTACGACGTGGCAGCTCTTGCTAATAACTTAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAGgtgataacat ... gtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG


Block sizes: 49 (upstream exon), 42 (downstream exon)
Score: 140

TGATGACTTCCGGGACAGGGATTACGACGTGGCAGCTCTTGCTAATAACTTAAGTCAGGCATTTCGTTATGGGATATATAGCAACGATGACATTGAAGAGgtgataacat ... gtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gacattctagagtactgctacatattattgatgcatgtttgttttgtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG
                         tattgat  putative branch site (score: 140)
                                         ttttgtc  putative PPT
                  tacatattattgat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgctaaccttttcatgtttgctttagtatttttctcgtaaatgtggacattctagagtactgctacatattattgatgcatgtttgttttgtcaatgcagGCACAAGGATCACTTGAACGAGATGATGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATG
tgctaac
- - - - - - - tgtttgc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ctgctac
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -atgcatg