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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagttggtatactgaaatccatttctcattaaagctgtgtatttgcactaacaagagtattcgatacttcaatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGTATTTACCACCATGTAGTTGGTGGTGTTCTAAACCCG

Basic information

species Zea mays
transcript GRMZM2G075153_T04
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G075153_T02 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os03g25940.1 (Oryza sativa), 3'ss of exon 6
gtgagttggtatactgaaatccat-ttctcattaaagctgtgtatttgcactaacaagagtattcgatacttcaatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGTATTTACCACCATGTAGTTGGTGGTGTTCTAAACCCG
|| |||   |  ||  || | | | | ||||||| |  | |  ||| ||  | |||||| ||||   |        ||   ||||||||||||| ||| ||||||||||||||||| ||||||||||||| || || || |||||||||||||||||||||||||| |||
gtaagtgtttggaccaaacttcctgtactcattacacatatagatt-gccttgacaagaatattttgt--------tttttagGTTATTGGAGGCTGCATCAGTGGCAGAGATGAGCTAGTTTCCAAAGTCCGCATCTATCACCATGTAGTTGGTGGTGTTCTAAATCCG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|47962545|gb|CN845254.1|CN845254
EST:     CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
EST: gi|78122335|gb|DV540719.1|DV540719
EST:     CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
EST: gi|60398293|gb|DN231109.1|DN231109
EST:     CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
EST: gi|4647443|gb|AI622518.1|AI622518
EST:     CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
EST: gi|76291941|gb|DV031509.1|DV031509
EST:     CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
EST: gi|60397682|gb|DN230498.1|DN230498
EST:     ACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: ACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
EST: gi|149094738|gb|EE180294.2|EE180294
EST:     CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
EST: gi|71419167|gb|DR804675.1|DR804675
EST:     CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: CTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
EST: gi|32849779|gb|CD989460.1|CD989460
EST:     TACATTGCTGGACACAATGAT                         GTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT
genomic: TACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 241

AAGGCATTAACTTTAGGTGCTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGTATTTACCACCATGTAGTTGGTGGTGTTCTAAACCCG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 456

AAGGCATTAACTTTAGGTGCTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGTATTTACCACCATGTAGTTGGTGGTGTTCTAAACCCG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 486

AAGGCATTAACTTTAGGTGCTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGTATTTACCACCATGTAGTTGGTGGTGTTCTAAACCCG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 350

AAGGCATTAACTTTAGGTGCTGACCTAGTTATTCATTCTGCAACGAAGTACATTGCTGGACACAATGATgtgagttggt ... aatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGTATTTACCACCATGTAGTTGGTGGTGTTCTAAACCCG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tcattaaagctgtgtatttgcactaacaagagtattcgatacttcaatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGTATTTACCACCATGTAGTTGGTGGTGTTCTAAACCCG
                    cactaac  putative branch site (score: 350)
                                               tttccc  putative PPT
                                          ttcaattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagttggtatactgaaatccatttctcattaaagctgtgtatttgcactaacaagagtattcgatacttcaatttcccagGTTATTGGAGGATGCGTCAGTGGCAGAGATGAGTTAGTTTCCAAAGTTCGTATTTACCACCATGTAGTTGGTGGTGTTCTAAACCCG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGTGG