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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatgtacccttttaacctaacagggttttgaatacttcaatcctttaaatttatgtgatttgggttggaatttgttctgagaagtttatactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCAAGGGCATACAAAGTGGATAAG

Basic information

species Zea mays
transcript GRMZM2G074466_T01
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G074466_T01 (Zea mays), 3'ss of exon 7
lower sequence: LOC_Os01g39380.3 (Oryza sativa), 3'ss of exon 27
--gtatgtacccttttaacctaacagggttttgaatacttcaatcctttaaatttatgtgatttgggttggaatttgttctgagaagtttatactca---ccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCAAGGGCATACAAAGTGGATAAG
  |||  ||     |   || ||  |  |||     | ||  |   |    | ||  |  | |||||||| |||||  | | | ||    |    ||    |||   ||||||||||||||||||||| ||||| ||||||||||||||||| |||||||| ||||||||||||||
cagta-ataaatggtgtcccaaagtgcatttcactcaattttaaattc---acttgagcaacttgggttgcaatttaatgtaaaaaaaaaaattccatcttcagGGTGGCACATGTCTGGGATATTCGTTCCAGTAAGCAGATGTTCAAGCTCCAAGGGCACACAAAGTGGATAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211489769|gb|FL012890.1|FL012890
EST:     ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATAT                         CACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
genomic: ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
EST: gi|32933778|gb|CF038590.1|CF038590
EST:     ATGGAATATTTTGTTTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATAT                         CACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
genomic: ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
EST: gi|67010825|gb|CO439574.1|CO439574
EST:     ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATAT                         CACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
genomic: ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
EST: gi|71437503|gb|DR818553.1|DR818553
EST:     ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATAT                         CACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTNCAAGCTTC
genomic: ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTT-CAAGCTTC
EST: gi|32850651|gb|CD990332.1|CD990332
EST:     ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTTCTGGAAGAGATAT                         CACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
genomic: ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
EST: gi|211489771|gb|FL012892.1|FL012892
EST:     ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATAT                         CACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
genomic: ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
EST: gi|211489770|gb|FL012891.1|FL012891
EST:     ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATAT                         CACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA
genomic: ATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 150

CTGATACTTGTGTTGTTACCGTGGGTCGTTGCCAAAGTGCTGTTCTCTGTATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCAAGGGCATACAAAGTGGATAAG


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 112

CTGATACTTGTGTTGTTACCGTGGGTCGTTGCCAAAGTGCTGTTCTCTGTATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCAAGGGCATACAAAGTGGATAAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 123

CTGATACTTGTGTTGTTACCGTGGGTCGTTGCCAAAGTGCTGTTCTCTGTATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCAAGGGCATACAAAGTGGATAAG


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 92

CTGATACTTGTGTTGTTACCGTGGGTCGTTGCCAAAGTGCTGTTCTCTGTATGGAATATGATGATTCCACTGGGATTCTGGCGGCTGCTGGAAGAGATATgtatgtaccc ... tactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCAAGGGCATACAAAGTGGATAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttaaatttatgtgatttgggttggaatttgttctgagaagtttatactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCAAGGGCATACAAAGTGGATAAG
                                      aagtttata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatgtacccttttaacctaacagggttttgaatacttcaatcctttaaatttatgtgatttgggttggaatttgttctgagaagtttatactcaccagCACGGCACATGTCTGGGATATTCGCTCCAGCAAGCAGATGTTCAAGCTTCAAGGGCATACAAAGTGGATAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGTGGA