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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatactactgtgaaatatggtgtcgttttacctttatcttctaatcgtccagcactctagccacaaaactagcaatatagttcacaagtgagtttgcctgtggatttatttctttccttattttttcggcataaatggtgctaagttgaccattcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTATTTTAAACTGGATCAGAGGATAGTCAATTTCTACACAAGAAATGGGGGAG

Basic information

species Zea mays
transcript GRMZM2G067299_T02
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G067299_T02 (Zea mays), 3'ss of exon 7
lower sequence: LOC_Os04g56580.1 (Oryza sativa), 3'ss of exon 7
gtatactactgtgaaatatggtgtcgttttacctttatcttctaatcgtcc-agcactctagccacaaaactagcaatatagttcacaagtgagtttgcctgt---ggatttatttctttccttattttttcggcataaatggtgctaagttgaccattcatt--tgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTATTTTAAACTGGATCAGAGGATAGTCAATTTCTACACAAGAAATGGGGGAG
                         || ||    | || |  |  | ||   || | |      |   | ||||     |     |||| ||  |||  || |     |  | ||| | |   | ||| ||   ||  ||      | | |||  ||||  ||  |||||||||||||  |||||||||||||||||||  ||||||||||||  ||||| |||||| ||||||| | |||||||||||||||||| |||||||| | ||
-------------------------gtattgatgtgatatgtttcttgtttgagaagttcgataagtcatctagttccttgtggaacaattgtttttttctttctaaaaactctttttattaatgtttctttcccaccaacc-cattgatttgcacatttgttgttgcagGCATATTTTATTGATCTGGATGTGAAACCCTTGGATAAGATGGTACATTACTTTAAATTGGATCAAAAGATAGTCAATTTCTACACTAGAAATGGAGAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60396151|gb|DN229020.1|DN229020
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|149086245|gb|EE175785.2|EE175785
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|60340715|gb|DN207688.1|DN207688
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|32805998|gb|CD958232.1|CD958232
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|60338305|gb|DN205278.1|DN205278
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|60398038|gb|DN230854.1|DN230854
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|88749203|gb|DY533344.1|DY533344
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|78112746|gb|DV531140.1|DV531140
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|71772064|gb|DR970001.1|DR970001
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|94477322|gb|EB706280.1|EB706280
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|94473866|gb|EB702824.1|EB702824
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|211510844|gb|FL479757.1|FL479757
EST:     TATGATTCCAGTGTTTCTTGAATCAAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: TATGATT-CAGTGTTTCTTGAATC-AGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
EST: gi|211182792|gb|FL070443.1|FL070443
EST:     AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAG                         GCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT
genomic: AGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 40

TAGCCTGATGATCAGCTTTCGGCCAAGAGAGAATGGTAGTACAGATTCTGAGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTATTTTAAACTGGATCAGAGGATAGTCAATTTCTACACAAGAAATGGGGGAG


Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 38

TAGCCTGATGATCAGCTTTCGGCCAAGAGAGAATGGTAGTACAGATTCTGAGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTATTTTAAACTGGATCAGAGGATAGTCAATTTCTACACAAGAAATGGGGGAG


Block sizes: 47 (upstream exon), 44 (downstream exon)
Score: 24

TAGCCTGATGATCAGCTTTCGGCCAAGAGAGAATGGTAGTACAGATTCTGAGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTATTTTAAACTGGATCAGAGGATAGTCAATTTCTACACAAGAAATGGGGGAG


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 27

TAGCCTGATGATCAGCTTTCGGCCAAGAGAGAATGGTAGTACAGATTCTGAGTATGATTCAGTGTTTCTTGAATCAGTGAAGCGAACCTATGAGTACAAGgtatactact ... tcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTATTTTAAACTGGATCAGAGGATAGTCAATTTCTACACAAGAAATGGGGGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttctttccttattttttcggcataaatggtgctaagttgaccattcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTATTTTAAACTGGATCAGAGGATAGTCAATTTCTACACAAGAAATGGGGGAG
                                   agttgac  putative branch site (score: 27)
                                            ttcattt  putative PPT
         ttatttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatactactgtgaaatatggtgtcgttttacctttatcttctaatcgtccagcactctagccacaaaactagcaatatagttcacaagtgagtttgcctgtggatttatttctttccttattttttcggcataaatggtgctaagttgaccattcatttgcagGCATATTTCCTTGATCTGGATGTGAAACCTCTGGATAAGATGGAGCATTATTTTAAACTGGATCAGAGGATAGTCAATTTCTACACAAGAAATGGGGGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAA