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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ccctttatccttaaacttgagtgtgtctagtatgtctggggttagatggatacaaggtatcgcacagtttgtactaatgatttacaattcccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCAAGAGCATTCAATCTCGGTCGCGTGTTTATCCATTTCTG

Basic information

species Zea mays
transcript GRMZM2G066362_T01
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G066362_T01 (Zea mays), 3'ss of exon 7
lower sequence: LOC_Os01g07720.3 (Oryza sativa), 3'ss of exon 6
ccctttatccttaaacttgagtgtgtctagtatgtctgggg----ttagatggatacaaggtatcgcacagtttgtactaatgatttacaattcccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCAAGAGCATTCAATCTCGGTCGCGTGTTTATCCATTTCTG
      ||    |   ||| |    | || |  |   |||     ||||||| |||    | ||| |||  ||  | |||||| || ||  |||| |||| ||||||||||||||||||||||||||| | |||||||||||||||||||||||| ||||||||||||| || || || || |||||||||||
----acatgaaaatgtttgtggtactttaatcagctagggagtgattagatgaatatgcagcatcacactattgatgctaatgcttcacgtttcctatctatagGTTTTGTTGGGTATGCCATTCTTGTTATCACATCCAGTTGAGTACATCTCACGAGCATTCAATCTTGGGCGTGTCTTCATCCATTTCTG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71446401|gb|DR827451.1|DR827451
EST:     TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAG                         GTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCA
genomic: TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCA
EST: gi|78103804|gb|DV522232.1|DV522232
EST:     TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAG                         GTTTTGTTGGGTATG
genomic: TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATG
EST: gi|76929771|gb|DV172155.1|DV172155
EST:     TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAG                         GTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCCGTTGAGTACCTCTCA
genomic: TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCA
EST: gi|148950403|gb|EC895970.2|EC895970
EST:     TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAG                         GTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCA
genomic: TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCA
EST: gi|50327840|gb|CO522966.1|CO522966
EST:     TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAG                         GTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCA
genomic: TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCA
EST: gi|149078217|gb|EE166971.2|EE166971
EST:     TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAG                         GTTTTGTTGGGTATGCCATTCTTGCTGT
genomic: TGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 93

GCCATGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCAAGAGCATTCAATCTCGGTCGCGTGTTTATCCATTTCTG


Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 63

GCCATGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCAAGAGCATTCAATCTCGGTCGCGTGTTTATCCATTTCTG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 107

GCCATGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCAAGAGCATTCAATCTCGGTCGCGTGTTTATCCATTTCTG


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 71

GCCATGAGCATCAAAGGAGTCTTCTTTGCCTTATTGGGAGCTGCTGTCGTACAGgtacattgtt ... ccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCAAGAGCATTCAATCTCGGTCGCGTGTTTATCCATTTCTG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atggatacaaggtatcgcacagtttgtactaatgatttacaattcccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCAAGAGCATTCAATCTCGGTCGCGTGTTTATCCATTTCTG
                          tactaat  putative branch site (score: 71)
                                          ttcccatct  putative PPT
                             taatgatttacaatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ccctttatccttaaacttgagtgtgtctagtatgtctggggttagatggatacaaggtatcgcacagtttgtactaatgatttacaattcccatctgtagGTTTTGTTGGGTATGCCATTCTTGCTGTCACATCCAGTTGAGTACATCTCAAGAGCATTCAATCTCGGTCGCGTGTTTATCCATTTCTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGCA
- - - - - - - - - - - - - - - - tgtctgg