ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...tcaaaattagctttccacactgttgtaccatattgacatttcatattcaactgaacttaaatgtttctgtatttatcattattatttgcccttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA

Basic information

species	Zea mays
transcript	GRMZM2G009845_T02
intron #	7
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G009845_T02 (Zea mays), 3'ss of exon 7
lower sequence: LOC_Os05g06350.1 (Oryza sativa), 3'ss of exon 7

---tcaaaattagctttccacactgttgtaccatattgacatttcatattcaactgaacttaaatgtt-tctgtatttatcattattattt--gcccttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA
   ||  ||||     |  ||   |||   |   |   | | || | ||   | |    ||| ||||| ||||  || ||  ||  | | |       | |||||||||||||| ||  ||||||| |||||||| || ||||||||  |||||| ||| || |||||||||||||||||||||||||||||||| ||||||||||
ctctctcaattgctgcttgactacgtt---ctcaagaaagaattgacataagatt---tttatatgttatctgggttcattgttcatctctcaattgctgctgcagTGTGTCATCGATCATCAAGCTCTTCCATGTCTCTTGAACCTCTTGACCAACAATCATAAGAAAAGCATCAAGAAAGAAGCATGCTGGACTATCTCAAACA

upper sequence: GRMZM2G009845_T02 (Zea mays), 3'ss of exon 7
lower sequence: PP1S165_54V6.1 (Physcomitrella patens), 3'ss of exon 7

tcaaaattagctttccacactgtt-gtaccatattga--catttcatattcaact-gaacttaaatgtttctgtatttatcattattatttgcccttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA
     | |    ||  | ||   | || ||| | ||   ||  |  | || |  | |  |||   |||  ||    |  |  || |  | |   |||    ||||   ||||  | ||||| || ||||| || ||| |    || ||||||| ||| ||||||||||| |||||||||||||| || ||||| || || || |
----gacttctattttataccactcgtcccagaatggttcaacttgtgttgactttgttcttgcttgtaactaacatccttcttgtattgttgtcttggatcagTTCATCATCAATAATCAGGCCCTTCCCTGTCTTCTTGCTCTTCTGACCAACAATCACAAGAAAAGTATCAAGAAAGAAGCCTGTTGGACTATTTCCAATA

upper sequence: GRMZM2G009845_T02 (Zea mays), 3'ss of exon 7
lower sequence: PP1S165_51V6.1 (Physcomitrella patens), 3'ss of exon 7

-----------------------------tcaaaattagctttccacactgttgtaccata-ttgacatttcatattcaactgaacttaaatgtttctgtattt-----------atcattattatttgc-----------ccttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA
                             ||    ||   |||| ||| |  || | || | |||    |   |||   || |   | |    ||  |  | ||           |||| || | ||| |           |  ||  |||||   |||| |||   || ||  | || |||||  |    ||| |||||| |||||||||||| ||||| |||||||||||||| ||||| ||||| ||||
gtatccatacaagatctgaaatcattgcttcgctgtttcttttcaacatttctgcatcaaagttgttcatgtgtatgttacggtcgtcacccattatttcactttccatcccgtcatcagtagtgtttacgtgcgtgtttactgtcgcgcagTTCATCATCGACTGCCAGGCCTTGCCCTGCCTGCTCGCACTATTGACCAACAACCACAAGAAGAGCATTAAGAAAGAAGCATGTTGGACTATCTCCAACA

upper sequence: GRMZM2G009845_T02 (Zea mays), 3'ss of exon 7
lower sequence: PP1S63_171V6.1 (Physcomitrella patens), 3'ss of exon 7

-------------------------------------tcaaaattagc---tttccacactgttgtaccatattgacatttcatattcaactgaacttaaatgttt--ctgtatttatcattattatttgcccttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA
                                     | |   || |    |||| ||  | ||  | | |  |        | | || |||      |  |  ||     | |  |    |    ||| ||| |   |||||   |||||||   |||||| || || || ||  |    ||||| |||| |||||||||||| || ||||| || |||||||||||||| || || ||||
gtaatttctcgatgcattctggccttttccatgtttttaagcctttgtccatttcgaccttcttccagcgttgtcctggcaaagagtccacttcttccagttcgttgataatgtcaactgcttacgttt-cccgttgcgcagTTCATCATTGATTGTCAAGCCCTACCGTGTCTGCTCGCACTACTCACCAACAACCACAAGAAGAGTATCAAAAAGGAAGCATGCTGGACCATTTCCAACA

upper sequence: GRMZM2G009845_T02 (Zea mays), 3'ss of exon 7
lower sequence: PP1S43_141V6.1 (Physcomitrella patens), 3'ss of exon 8

tcaaaattagctttccacac-tgttgtaccatattgacatttcatattcaactgaac--ttaaatgtttctgtatttatcattattatttgcccttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA
 |    || | |  | | || |||| |   | ||| |  |     | | |   | ||      |  ||| |||| ||  |  ||||  |     ||    ||||   ||||  ||||||| || || || || ||| |    || || |||| |||||||||||||||||| || || ||||| |||||||| ||||| ||||
cctctttttggtagcaatacgtgttttttaaaattca-gtaaggaaataagggggacggcctgaaatttgtgtactttcctctattgctg--ttttgtatcagTTCATCATCAACAATCAGGCCCTCCCGTGTCTTCTCGCTCTTCTCACCAACAACCACAAGAAAAGCATTAAAAAGGAAGCCTGCTGGACTATCTCCAACA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211452352|gb|FL292617.1|FL292617

EST:     TCTGCGCACGGTGGGTAACATCTGTAACTGGNGACGACATGCANACTCAG                         TGTGNCNTTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC
genomic: TCTGCGCACGGTGGGTAACATC-GTAACTGGGGACGACATGCAGACTCAGgtaaactcaa ... cttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC

EST: gi|71322679|gb|DR798011.1|DR798011

EST:     CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAG                         TGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC
genomic: CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAGgtaaactcaa ... cttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC

EST: gi|31350199|gb|CD434556.1|CD434556

EST:     CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAG                         TGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC
genomic: CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAGgtaaactcaa ... cttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC

EST: gi|32848180|gb|CD987861.1|CD987861

EST:     CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAG                         TGTGTCATTGACAATCAAGCACTCCCATGCCTTTTGAACCTACTGACCACC
genomic: CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAGgtaaactcaa ... cttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC

EST: gi|32929275|gb|CF034087.1|CF034087

EST:     CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAG                         TGTGTCATTGACAATCAAGCACTCCCATGCCTTTTGAACCTACTGACCACC
genomic: CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAGgtaaactcaa ... cttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC

EST: gi|32928651|gb|CF033463.1|CF033463

EST:     CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAG                         TGTGTCATTGACAATCAAGCACTCCCATGCCTTTTGAACCTACTGACCACC
genomic: CTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAGgtaaactcaa ... cttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 410

GCATCCTTCAGCCTCTGTACTGATTCCAGCTCTGCGCACGGTGGGTAACATCGTAACTGGGGACGACATGCAGACTCAGgtaaactcaa ... cttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 371

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 322

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 402

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttcaactgaacttaaatgtttctgtatttatcattattatttgcccttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA

                                    ttatttgcccttcct  CT-rich tract
        aacttaaatgtttctg  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tcaaaattagctttccacactgttgtaccatattgacatttcatattcaactgaacttaaatgtttctgtatttatcattattatttgcccttcctgcagTGTGTCATTGACAATCAAGCACTTCCATGCCTTTTGAACCTACTGACCACCAACCACAAGAAAAGCATCAAGAAAGAAGCATGCTGGACAATCTCAAACA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ATGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGGA
- - - - - - - - - -ctgttgt
- - - - - - - - - - - - - - - - -tgacatt
- - - - - - - - - - - - - - - - - - - - - - - - aactgaa