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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacgtagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG

Basic information

species Zea mays
transcript GRMZM2G004528_T03
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: LOC_Os03g09250.2 (Oryza sativa), 3'ss of exon 7
gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacgtagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
|| ||  |  ||  | |  |    | |  |    |    | |  |  |    |||| ||| |||  ||| |||| || |  |   ||  |   ||||||||||||||||||| |||||||| |||||||| |||||||||||||| ||||||||||||||||| || |||||||||||||| |||||||||||||| |
gtttgcaatttgtccatggattcttctgcagcagtatgccattaaaaaa---aaacttagttttggattaaaacttgtccatgtattcttc--tgcagTATGTGCCGTATGTTGGAGACAGCAAGAGGGCAATGGACGAGTACACCTCAGAGATCTTCATGGGGGGTAAGAGCACCATCGTTCTGCACAACACCTGTG

upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: GLYMA18G02210.3 (Glycine max), 3'ss of exon 7
gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcag---tttaaacgtagatttacattg--aaacctgccactc------ctttgctgcat--gcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
||       | || | |     | ||   || |       ||    |     ||| |   |   |||   |||  |   ||   | ||      ||||| ||| |  |||||||||||| || || || || || ||||| || ||||| |||||||| |||||||| ||||||||                                   
gtgaacatcccgaccatgttgttgttattaaggtatatttgtttcacccatttttcactatttctttctcttgtcagGTCTTTGATTCTATCTGCTTTGTTGCCTTTGCAGTATGTGCCTTACGTAGGGGATAGCAAGAGAGCCATGGATGAGTACACTTCAGAGATATTCATGGGT----------------------------------

upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: GLYMA08G14670.3 (Glycine max), 3'ss of exon 6
gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacgtagatttacattgaaacct--gccactcctttgctg--catgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
             |  |     | ||||   | ||  | |       | || ||  |    ||||  ||||  | |  |  | |  |||  ||    |||||||||| || || || || ||||| |||||||| ||||| |||||| | || ||||| ||||||||||| |||| ||| || ||| |||||||||| || |
------------gtaatttttatttttagtca-ctcactctctttcaaattcaattggttctttat-ttgactcttcggtaattaatttattggttttgcagTATGTACCTTACGTTGGGGACAGCAAGAGGGCCATGGATGAGTACTCTTCTGAGATATTCATGGGCGGAAAGAACACAATTGTGTTGCACAACACTTGTG

upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: GLYMA18G02210.4 (Glycine max), 3'ss of exon 7
gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacgtagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
|| | || | |  ||  |||  | |    |   ||  | | ||               || | |   ||||  |   |  ||   ||||    ||||||||||||| || || || || || ||||| || ||||| |||||||| |||||||| |||||||| || |||| |||||| ||  |||||||||| || |
gtatatttgtt--tcacccatttttcactatttctttctcttgt-------------caggtct---ttgattctatctgctttgttgcctt-tgcagTATGTGCCTTACGTAGGGGATAGCAAGAGAGCCATGGATGAGTACACTTCAGAGATATTCATGGGTGGAAAGAACACCATTGTTTTGCACAACACATGTG

upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: AT5G10170.1 (Arabidopsis thaliana), 3'ss of exon 7
gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacgtagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
      |   | || || |   |  ||| ||   |  |   |  | ||  | | |      || ||||  ||   | | | |   | | ||  ||||||||||||| | ||| |||||||| ||||| || ||||| || |||||||||||||| |||||||| || |||| ||| || ||| |||||||||| || |
-----gtgattaattttactattaattataa--ttatccaatctaacaaatcaca------ttaacat-aaattatactattggat-ggtgt-tgcagTATGTGCCATGTGTAGGAGACAGCAAGAGAGCAATGGATGAATACACATCAGAGATATTCATGGGAGGGAAGAACACGATTGTGATGCACAACACTTGTG

upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: AT4G39800.1 (Arabidopsis thaliana), 3'ss of exon 7
gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacgtagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
      |||   | |   ||   |  | |||| |||   |     ||| | | |     |||   ||||||  |          |||| |   || ||||||| |||||||| |  || |||||| | || ||||||||||||||||||||||| |||||||| || |||| ||| || ||| ||||||| |||||||
-gtaaatagagaaccggacaga-cactcaaaacctgtttctgttggcaatctga-----gatcc-cattgatccaatttgtgtgtttgtt--ttgtagTATGTACCGTATGTTGCGGATAGTAAGCGAGCCATGGACGAGTACACATCAGAGATATTCATGGGAGGGAAGAACACAATTGTGATGCACAATACCTGCG

upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: Vv07s0031g00920.t01 (Vitis vinifera), 3'ss of exon 7
gtctgttagctgatcttccacctctttaaaaagctggca---cgtgcagcagtttaaacgtag--atttacattga--aacctgccactcctttgctgca--tgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
|| | |      | | |||   | |||  |    |  ||   |        ||||  ||  |   |||  || ||   ||| |   ||    ||| ||    ||||||||||||| ||||| ||||| || ||||| || ||||| |||||||| || ||||| |||||||| || ||||||||||| |||||||||||||||||||
gtatatcgcagaaccctccttttgtttgtatgtattacaattcagatgctggtttggactcattcattctcactggctaacattatacggtgttgttgatattgcagTATGTGCCATATGTTGGAGATAGCAAGAGAGCCATGGATGAGTACACTTCTGAGATATTCATGGGTGGGAAGAGCACCATTGTGCTGCACAACACCTGCG

upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: PP1S55_204V6.1 (Physcomitrella patens), 3'ss of exon 6
----------------------------------gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacg-----tagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
                                  || |   |||   |    |     ||| | |  |      || | ||| |   | ||        || | | |   ||||     |      |  |  ||||||| || || |||||||| ||||| |||||||| |||||||||||||| || |||||||||||||||||    | ||| |||||  ||||||| || ||||
gtattcttatctctactccagccctgcagtcaatgttttcaagccatttcggctttgatttcagactccaagttgt-ctgcattcacaccgattctcgaatcttcctcttaaccaaaatcaatgacggcgtctgcagTACGTCCCTTATGTGGGTGACAGCAAGAGGGCAATGGACGAGTACACTTCGGAGATCTTCATGGGCGGACGTAACACAATCGTCATGCACAATACGTGCG

upper sequence: GRMZM2G004528_T03 (Zea mays), 3'ss of exon 7
lower sequence: EFJ37320 (Selaginella moellendorffii), 3'ss of exon 7
gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacgtagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
                             ||| |   | | ||      ||  |  | | | |  |     |   |||   || ||| ||      ||||||| || || || || || || ||| |||| |||||||| ||||| || ||||| |||||||| |||| |||||||||||   |||||||||| ||||
---------------------------gtaaaacaaacgcttgatttttttctagggaaaactcccg----agtttgct-ttcgttttct------agTATGTTCCTTACGTCGGTGATAGCAAGCGGGCCATGGACGAATACACGTCCGAGATATTCATGGGAGGCAGGAGCACCATCGCCATGCACAACACTTGCG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32855391|gb|CD995072.1|CD995072
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TACGTGCCGTACGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|32856301|gb|CD995982.1|CD995982
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TACGTGCCGTACGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|110941538|gb|EE161695.1|EE161695
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|32855713|gb|CD995394.1|CD995394
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TACGTGCCGTACGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|6637095|gb|AW261367.1|AW261367
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|6021766|gb|AW066694.1|AW066694
EST:     TGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGNCAGTAA-AGGGCTA-GG-CGAGTAC-C-TCA
genomic: TGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|71756431|gb|DR954368.1|DR954368
EST:     AGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: AGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|91871596|gb|EB401553.1|EB401553
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|71771141|gb|DR969078.1|DR969078
EST:     ATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: ATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|6527007|gb|AW216231.1|AW216231
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|6637051|gb|AW261323.1|AW261323
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGNTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|32854168|gb|CD993849.1|CD993849
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TACGTGCCGTACGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|149071426|gb|EE159821.2|EE159821
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|211029038|gb|FL245284.1|FL245284
EST:     CMATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TACGTGCCGTACGTTGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|32921535|gb|CF026347.1|CF026347
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTA
EST: gi|31364344|gb|CD448699.1|CD448699
EST:     CGGACTTCATCGCCGCTAACCAGGGCTCTCGTGCCAACAATGTCATCAAGGGCACCAAGAAAGAACAGGTGGAGCAGATCATCAAAGATATCAG
genomic: CGGACTTCATCGCCGCTAACCAGGGCTCTCGTGCCAACAATGTCATCAAGGGCACCAAGAAAGAACAGGTGGAGCAGATCATCAAAGATATCAG
EST: gi|32945128|gb|CF049947.1|CF049947
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TACGTGCCGTACGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|60397539|gb|DN230355.1|DN230355
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|148952105|gb|EC897897.2|EC897897
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|60346856|gb|DN213829.1|DN213829
EST:     ACCAGGGCTCTCGTGCC-ACAATGTCATCAAGGGCACCAAGAAAGAACAGGTGGAGCAGATCATCAAAGATATCAG
genomic: ACCAGGGCTCTCGTGCCAACAATGTCATCAAGGGCACCAAGAAAGAACAGGTGGAGCAGATCATCAAAGATATCAG
EST: gi|21621348|gb|BQ619354.1|BQ619354
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|32854394|gb|CD994075.1|CD994075
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TACGTGCCGTACGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|148928580|gb|EC873008.2|EC873008
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCA
EST: gi|32920511|gb|CF025323.1|CF025323
EST:     CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAG                         TATGTGCCGTA
genomic: CCATTCTCTATGGGCCCGGCGAGCATCCCGATCATGTTGTTGTCATCAAGgtctgttagc ... ctgcatgcagTATGTGCCGTA
EST: gi|67029746|gb|CO458495.1|CO458495
EST:     CGGACTTCATCGCCGCTAACCAGGGCTCTCGTGCCAACAATGTCATCAAGGGCACCAAGAAAGAACAGGTGGAGCAGATCATCAAGGATATCAG
genomic: CGGACTTCATCGCCGCTAACCAGGGCTCTCGTGCCAACAATGTCATCAAGGGCACCAAGAAAGAACAGGTGGAGCAGATCATCAAAGATATCAG


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cagcagtttaaacgtagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG
                                     ctcctttgct  CT-rich tract
                 atttacatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtctgttagctgatcttccacctctttaaaaagctggcacgtgcagcagtttaaacgtagatttacattgaaacctgccactcctttgctgcatgcagTATGTGCCGTATGTGGGAGACAGTAAGAGGGCTATGGACGAGTACACATCAGAGATCTTCATGGGCGGCAAGAGCACCATCGTGCTGCACAACACCTGCG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC