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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gaataatataactggatctggatgcggcaaattttgcctcgtctttatggactgtaattcatatgttctaacagcgtattttgatgttttttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTATGGATCCTAGGCACCCTGAG

Basic information

species Zea mays
transcript GRMZM5G834205_T02
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G834205_T02 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os08g01740.2 (Oryza sativa), 3'ss of exon 6
--------gaataatataactgg-atctggatgcggcaaattttgcctcgtctttatggactgtaattcatatgttctaacagcgtattttgatgtttttt-cagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTATGGATCCTAGGCACCCTGAG
        || |   || | |   | ||   || |  ||   ||     | |||| |||  |    ||||   ||| | |||| ||| |||| | | | || ||||||| | | |||||| |||||||||||||||||  | | ||| |||||| |||| ||||||||||| |||||||||
ggtgtcacgattgtgatgattattacctctttgtgttaaccattcaactgccttt-tggctt----ttcaatcgtttttacagtgtactttggtttatattgcagTATAATTCGGAAAGCTTCTAGTGTCATGGAAGTTCTAAAGGACGTGCTTGATTCGATGGATCCTAGACACCCTGAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149079652|gb|EE168649.2|EE168649
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|93015304|gb|EB640824.1|EB640824
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|93015622|gb|EB641142.1|EB641142
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|149083864|gb|EE173239.2|EE173239
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|71428013|gb|DR809063.1|DR809063
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|9953455|gb|BE640038.1|BE640038
EST:     TTGACTGAAGCTCAACAGGAAGTGCATACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGNCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|78107222|gb|DV525640.1|DV525640
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTA
EST: gi|71421501|gb|DR805467.1|DR805467
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTA
EST: gi|211276223|gb|FL230638.1|FL230638
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|149102741|gb|EE288090.2|EE288090
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|71320275|gb|DR796886.1|DR796886
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|76291818|gb|DV031386.1|DV031386
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|76291817|gb|DV031385.1|DV031385
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|78116688|gb|DV535075.1|DV535075
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|76282167|gb|DV021735.1|DV021735
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|101391432|gb|EB817651.1|EB817651
EST:     GAAAGCTCAACAGGAAGGGCACACTCAGCCTGTTCCGGATGCAAGTATAG                         TCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCT
genomic: G-AAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCT
EST: gi|76924364|gb|DV169917.1|DV169917
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCT
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCT
EST: gi|148952812|gb|EC898804.2|EC898804
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|32837204|gb|CD976882.1|CD976882
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|76021099|gb|DT948269.1|DT948269
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|71326004|gb|DR799684.1|DR799684
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|149105966|gb|EE291599.2|EE291599
EST:     TGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCTAGTATAG                         TCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCT
genomic: TGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC-A--A--Ggtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCT
EST: gi|76924362|gb|DV169916.1|DV169916
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|89249854|gb|DY621640.1|DY621640
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|148938286|gb|EC882590.2|EC882590
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|71428014|gb|DR809064.1|DR809064
EST:     TGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGTCAGTATAG                         TCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTTATTCT
genomic: TGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATG-CA--A--Ggtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCT
EST: gi|86472430|gb|DY238800.1|DY238800
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|149085134|gb|EE174639.2|EE174639
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|76927739|gb|DV171347.1|DV171347
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|149088092|gb|EE287276.2|EE287276
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|71326005|gb|DR799685.1|DR799685
EST:     TGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGTCAGTATAG                         TCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGAT
genomic: TGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATG-CA--A--Ggtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGAT
EST: gi|149059924|gb|EE157051.2|EE157051
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|84970062|gb|DW468434.1|DW468434
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|74245587|gb|DT653501.1|DT653501
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|78091790|gb|DV520164.1|DV520164
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|149085242|gb|EE174748.2|EE174748
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|50339159|gb|CO534285.1|CO534285
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|76281749|gb|DV021317.1|DV021317
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|71421504|gb|DR805468.1|DR805468
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTA
EST: gi|149105965|gb|EE291598.2|EE291598
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAA
EST: gi|48690727|gb|CO049262.1|CO049262
EST:     GCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: GCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt
EST: gi|149095623|gb|EE174749.2|EE174749
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAG                         TATAGTCCAGAAAGCCTCTAGTGT
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagT-----CCAGAAAGCCTCTAGTGT
EST: gi|93017402|gb|EB642922.1|EB642922
EST:     TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGTATAG                     
genomic: TTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGC----A-AGgtactttctt ... ttcagt

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 2 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 6

CAGTCCCAGAAACGAGAACAGAACCAAATAATGAAAGTTTATCGACCCGTTTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTATGGATCCTAGGCACCCTGAG


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 10

CAGTCCCAGAAACGAGAACAGAACCAAATAATGAAAGTTTATCGACCCGTTTGACTGAAGCTCAACAGGAAGTGCACACTCAGCCTGTTCCTGATGCAAGgtactttctt ... ttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTATGGATCCTAGGCACCCTGAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tatggactgtaattcatatgttctaacagcgtattttgatgttttttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTATGGATCCTAGGCACCCTGAG
                                       tgttttttc  CT-rich tract
                               tattttgatgtttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gaataatataactggatctggatgcggcaaattttgcctcgtctttatggactgtaattcatatgttctaacagcgtattttgatgttttttcagtatagTCCAGAAAGCCTCTAGTGTCATGGAAGTATTGAGGGATGTGCTTAATTCTATGGATCCTAGGCACCCTGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAAG
- - - - - -ctggatc
- - - - - - - - - tggatgc
- - - - - - - - - - - - - - - - - tgcctcg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttcatat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgatgtt