ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...agatactttcatccttgctacgtgtttcctaacatgaattggtagatagagcttcgcctgttattttcaattgtagttgatatatccctataactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCTTTTGTGCCAAAGGAAAAACACCTTGGGAGGATGTCAAAAGAGAGTATGT

Basic information

species	Zea mays
transcript	GRMZM2G468693_T05
intron #	6
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G468693_T03 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os04g44890.1 (Oryza sativa), 3'ss of exon 6

agatactttcatccttgctacgtgtttcctaacatgaattggtagatagagcttcgcctgttattttcaattg--tagttgatatatccctataactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCTTTTGTGCCAAAGGAAAAACACCTTGGGAGGATGTCAAAAGAGAGTATGT
    | |   |   ||| ||| ||     |    || ||||  |||||    ||    ||||  |||  ||||  | ||   |  ||  ||  |||||  ||||||||| ||||| |||||||| |||||||| |||||||||||||| || |||||| |||||||||||||||||||||||||||||||||||||||| ||
--gaattgctactgttgttacttggagagttcattgcattgccagatattattttttgtgtttctttttattggtttgtatcttgatttctgcaactgccagGTGCCGCTTCTGTAATGGTTGCCAGAGGTGCCATGTGGAATGCCTCAATTTTTTGTCCCAAAGGAAAAACACCTTGGGAGGATGTCAAAAGAGAGTACGT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|78022286|gb|DV490673.1|DV490673

EST:     TGGAGATGTCTTTGAGTATGAGGATTTTAAGAGAATTAAAGATGCAACAG                         GTGCCGCGTCTGTTATGGTTGCTAGAGGTGCCATGTGGAATGCCTCCATCT
genomic: TGGAGATGTCTTTGAGTATGAGGATTTTAAGAGAATTAAAGATGCAACAGgtttttgttt ... taactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT

EST: gi|211207814|gb|FL427954.1|FL427954

EST:     TGAGGATTTTAAGAGAATTAAAGATGCAACAG                         GTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT
genomic: TGAGGATTTTAAGAGAATTAAAGATGCAACAGgtttttgttt ... taactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT

EST: gi|211300563|gb|FL370635.1|FL370635

EST:     TGAGGATTTTAAGAGAATTAAAGATGCAACAG                         GTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT
genomic: TGAGGATTTTAAGAGAATTAAAGATGCAACAGgtttttgttt ... taactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT

EST: gi|211529896|gb|FL379285.1|FL379285

EST:     TGAGGATTTTAAGAGAATTAAAGATGCAACAG                         GNGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT
genomic: TGAGGATTTTAAGAGAATTAAAGATGCAACAGgtttttgttt ... taactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT

EST: gi|76015755|gb|DT942925.1|DT942925

EST:     TGGAGATGTCTTTGAGTATGAGGATTTTAAGAGAATTAAAGATGCAACAG                         GTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT
genomic: TGGAGATGTCTTTGAGTATGAGGATTTTAAGAGAATTAAAGATGCAACAGgtttttgttt ... taactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 50

GATGAGATAGCTGATGTTGTGTCAGCGCTGTCAATTCCAGTTATAGCTAATGGAGATGTCTTTGAGTATGAGGATTTTAAGAGAATTAAAGATGCAACAGgtttttgttt ... taactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCTTTTGTGCCAAAGGAAAAACACCTTGGGAGGATGTCAAAAGAGAGTATGT

Block sizes: 46 (upstream exon), 46 (downstream exon)
Score: 47

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 93

Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 54

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

atagagcttcgcctgttattttcaattgtagttgatatatccctataactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCTTTTGTGCCAAAGGAAAAACACCTTGGGAGGATGTCAAAAGAGAGTATGT

                                          ctataac  putative branch site (score: 54)
                                       tccctat  CT-rich tract
               ttattttcaattgta  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agatactttcatccttgctacgtgtttcctaacatgaattggtagatagagcttcgcctgttattttcaattgtagttgatatatccctataactgttagGTGCCGCGTCTGTTATGGTTGCTAGAGGTGCTATGTGGAATGCCTCCATCTTTTGTGCCAAAGGAAAAACACCTTGGGAGGATGTCAAAAGAGAGTATGT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGA