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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttgcgctttttagggacacgtcaaagtgctcatttttgggttgtatattcatgtttcaaatattagtatagtactcacattcacctttcattgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG

Basic information

species Zea mays
transcript GRMZM2G451483_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G451483_T01 (Zea mays), 3'ss of exon 6
lower sequence: GLYMA14G10950.1 (Glycine max), 3'ss of exon 5
-----ttgcgctttttagggacacgtca-aagtgctcatttttgggttgtatattcatgtttcaaatattagtatagtactcacattcacctttcattgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG
     || |  ||| || |      ||   | |||  ||  | || |||||  ||    | |||    |   | || |  |   | |    || | ||||| | |||| |||||  | |||||||| |||||||| ||||| |||||||| || || |||||||| ||||||||||||||| | ||||| ||||| ||||| || |
ctttgttacattttatatgcttctttcctgactgcatatagt-ggcttgtacttttccttatca----tctttgtaattgtatgactttttttcccttgtgac-agCGCTTTACTCGTCTTGGTGGTAAGCTTCCAAAAGGAGTTTTGCTTGTTGGTCCACCTGGCACTGGAAAAACTATGTTAGCAAGAGCTATTGCTGGAGAGG

upper sequence: GRMZM2G451483_T01 (Zea mays), 3'ss of exon 6
lower sequence: GLYMA14G10960.1 (Glycine max), 3'ss of exon 3
-----ttgcgctttttagggacacgtca-aagtgctcatttttgggttgtatattcatgtttcaaatattagtatagtactcacattcacctttcattgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG
     || |  ||| || |      ||   | |||  ||  | || |||||  ||    | |||    |   | || |  |   | |    || | ||||| | |||| |||||  | |||||||| |||||||| ||||| |||||||| || || |||||||| ||||||||||||||| | ||||| ||||| ||||| || |
ttttgttacattttatatgcttctttcctgactgcatatact-ggcttgtacttttccttatca----tctttgtaattgtatgactttttttcccttgtgac-agCGCTTTACTCGTCTTGGTGGTAAGCTTCCAAAAGGAGTTTTGCTTGTTGGTCCACCTGGCACTGGAAAAACTATGTTAGCAAGAGCTATTGCTGGAGAGG

upper sequence: GRMZM2G451483_T01 (Zea mays), 3'ss of exon 6
lower sequence: AT2G26140.1 (Arabidopsis thaliana), 3'ss of exon 5
----------------------------------------------------------------------ttgcgctttttagggaca-cgtcaaagtgctcatttt-tgggttgtatattc---atgtttcaaatattagta------------tagtactcaca--ttcacctttcattgtgccc---agCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG
                                                                       |      | |   |  | | |  |     | ||  | ||  || | | | |    | |||| |   ||||||            |||  |||||   |||| ||||   | |   |   || | || ||  | || || |||||||||||||| ||||| ||||| || || || ||||| || |||||||||||||| || || ||||| ||||| ||||
gtaaattcacagtgctagagtgtttttctagttaagtataaaatctgtctagtgatgtaaattcaaaagcataaatcatgtgaagttatcatatagacctttatcctctgaattatctgtgccaggtttttctagagttagtactgttttgttcttaggcctcactctttcatcttttgatctttactatagAGGTTCACTCGTCTGGGAGGAAAGCTTCCTAAGGGTGTGTTGCTTGTCGGTCCTCCTGGGACAGGAAAAACTATGCTTGCTAGAGCTATTGCTGGAGAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71304073|gb|DR788158.1|DR788158
EST:     AAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAG                         CGTTTTACATGCC
genomic: AAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGCC
EST: gi|76283904|gb|DV023472.1|DV023472
EST:     AAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAG                         CGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGA
genomic: AAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGA
EST: gi|71439745|gb|DR820795.1|DR820795
EST:     AGCTAAAGCTGAACTTGAGGAAATTTGT-CACTACCTACGAGATCCTAAG                         CGTTTTACATGC
genomic: AGCTAAAGCTGAACTTGAGGAAATT-GTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGC
EST: gi|74238940|gb|DT646854.1|DT646854
EST:     AAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAG                         CGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGA
genomic: AAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGA
EST: gi|93283938|gb|EB676202.1|EB676202
EST:     AAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAG                         CGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGA
genomic: AAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 45 (downstream exon)
Score: 82

GCCCAACATGGATTCGAAGACAAAGTTCAGTGATGTCAAGGGAGTTGACGAAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 37

GCCCAACATGGATTCGAAGACAAAGTTCAGTGATGTCAAGGGAGTTGACGAAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG


Block sizes: 44 (upstream exon), 47 (downstream exon)
Score: 27

GCCCAACATGGATTCGAAGACAAAGTTCAGTGATGTCAAGGGAGTTGACGAAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 129

GCCCAACATGGATTCGAAGACAAAGTTCAGTGATGTCAAGGGAGTTGACGAAGCTAAAGCTGAACTTGAGGAAATTGTTCACTACCTACGAGATCCTAAGgtgaattgtt ... ttgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tattcatgtttcaaatattagtatagtactcacattcacctttcattgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG
                          tactcac  putative branch site (score: 129)
                                      cctttcatt  putative PPT
        tttcaaatattagtat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttgcgctttttagggacacgtcaaagtgctcatttttgggttgtatattcatgtttcaaatattagtatagtactcacattcacctttcattgtgcccagCGTTTTACATGCCTTGGTGGAAAGCTTCCTAAAGGTGTTTTGCTGGTGGGACCACCTGGAACTGGAAAAACTATGCTGGCAAGGGCTATAGCTGGGGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ATGCTG
- - - - - - - - - - - - - tgctcat
- - - - - - - - - - - - - - - - - - - - - - - attcatg