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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgctatggtttggcatggtcttggtaagttggatttgcaagttctgatgtgcttccctagtcctctttaaatgaagctgatggttgtttttcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG

Basic information

species Zea mays
transcript GRMZM2G386991_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G386991_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os01g62080.1 (Oryza sativa), 3'ss of exon 8
tgctatggtttggcatggtcttg--gtaagttggatttgcaagttc---tgatgtgcttccctagtcctctttaaatgaagctgatggttgtttttcc------tctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG
 |      ||  |     |  ||  ||  ||||| | ||||| ||    |  |||  |  | |   |||      || |  || ||| || ||||         | |  ||||||||||||| |||||||||||||||| |||||||| || || ||||||||||||||||||||||| |||||| |||||||||||||||||
-gtgggtattcagttaatttctgctgtttgttggttatgcaaattaacgttttgttttatcttgcccctaacagtattattctaatgtttattttattgtaatatttgcagTTATGCATGACCTGCGACTTATTCACACAGATCTGAAACCTGAGAACATTCTCCTTGTTTCATCAGAATCTATCCGGGTGCCTGATTATAAG

upper sequence: GRMZM2G386991_T01 (Zea mays), 3'ss of exon 6
lower sequence: GLYMA03G33100.1 (Glycine max), 3'ss of exon 6
---tgctatggtttggcatggtcttggtaagttggatttgcaagttctgatgtgcttccctagtcctctttaaatgaagctgatggt-tgtttttcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG
        |   ||   || |  |||| |  ||    ||  |   |  |||    ||    | |  | | |  | |   || || || | | |     ||  |||||||||||| ||  |  | |||||||| ||| |||||||||| |||||||| ||| |||||||||| |  || |  ||||| || ||||||
aaacaaactaaattattattgaattggcacatt----ttagataattagataattttggtcacttgtatcttcaagtcacttattgtgtctctcatacctgcagTTATGCATGATTTATGCTTGATTCACACTGATTTGAAGCCAGAGAACATTCTTCTTATTTCATCAGAATTCATTAAAGTGCCAGACTATAAG

upper sequence: GRMZM2G386991_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv08s0040g02640.t01 (Vitis vinifera), 3'ss of exon 6
tgctatggtttggcatggtcttggt-aagttggatttgcaagttctgatgtgcttccctagtcctctttaaatgaagctgatggttgtttttcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG
   ||     ||  |   | ||| |  |  | |  | |  | || | ||      ||| |   ||  |  | |||  |  ||   | | ||  |  |  |||||||||||| || || ||||| ||||| ||||||||||||||||| ||||| |||||||| ||||| |  ||||  ||||| |||||||||
gtttaaaacatgaaacactattgtttgaactagcatggtgaatt-taatagtaaaccccacatctgatccactgattcacatcactttgttatccttgcagTTATGCATGATTTACGCCTTATCCACACTGATCTGAAGCCAGAAAATATTCTTCTTGTTTCTTCAGATTACATCAAAGTGCCAGATTATAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211512137|gb|FL430403.1|FL430403
EST:     TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCAT                         TTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAGAACATTC
genomic: TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTC
EST: gi|32866970|gb|CF006652.1|CF006652
EST:     TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCAT                         TTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAGAACATTC
genomic: TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTC
EST: gi|149089547|gb|EE178794.2|EE178794
EST:     TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCAT                         TTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTC
genomic: TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTC
EST: gi|6095292|gb|AW119959.1|AW119959
EST:     TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCAT                         TTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTC
genomic: TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTC
EST: gi|6695913|gb|AW289021.1|AW289021
EST:     TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCAT                         TTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTC
genomic: TATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 154

GGGCCAAGCCTATATGACTTTCTGCGAAAGAATAGCTACCGTTCATTTCCTATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 161

GGGCCAAGCCTATATGACTTTCTGCGAAAGAATAGCTACCGTTCATTTCCTATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 179

GGGCCAAGCCTATATGACTTTCTGCGAAAGAATAGCTACCGTTCATTTCCTATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 193

GGGCCAAGCCTATATGACTTTCTGCGAAAGAATAGCTACCGTTCATTTCCTATTGACCTTGTCCGAGAATTTGCCAGACAAATATTAGAGTCTGTTGCATgtgagtactc ... tcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gatgtgcttccctagtcctctttaaatgaagctgatggttgtttttcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG
                                      ttgtttttcctctat  CT-rich tract
                    tttaaatgaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgctatggtttggcatggtcttggtaagttggatttgcaagttctgatgtgcttccctagtcctctttaaatgaagctgatggttgtttttcctctatagTTATGCATGACTTGCGACTTATTCACACGGATCTGAAGCCAGAAAACATTCTCCTTGTTTCATCAGAGTCTATCAGGGTGCCTGATTATAAG

tgctatg
- - - - - - gcatggt
- - - - - - - - - - - - - - - - atttgca
- - - - - - - - - - - - - - - - - - - - - -ctgatgt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gctgatg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - gttgttt