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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...attattgtatgtatgttatattctccaatattggggactgtatgagtgtataggtgttgacctctttgttagcctttacttattgatgttctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT

Basic information

species Zea mays
transcript GRMZM2G381744_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G381744_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os03g59020.1 (Oryza sativa), 3'ss of exon 6
---attattgtatgtatgttatattctccaatattgggg-actgta-tgagtgtataggtgttgacctctttgttagcctttacttattgatgttctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT
   | | ||| |||| ||  |  |    |||  |||||  |||||| ||| | |      |||    | |    |||   ||||   |  ||    | |||||||||| |||||||| | |||||| || || |||||||| ||||| || ||||||||||| ||| |  ||||| ||
tctaatgttg-atgt-tgaaaggtgtctcaaagttgggtcactgtaatgattatgattaggttctgttttacagtagtacttaccattctat---ttatttgcagGCTAATGGTTCTTAGAATCAGTTCAAAATTTGAACTGAGAAGATTTTGCCGTACTACAGGGTCCATTGCACTT

upper sequence: GRMZM2G381744_T01 (Zea mays), 3'ss of exon 6
lower sequence: GLYMA16G26920.1 (Glycine max), 3'ss of exon 6
---------attattgtatgtatgttatattctccaatat---tggggactgtatgagtgtataggtgttgacctctttgttagcctttacttattgatgtt-------ctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT
          |  || |   | | |  |  | |  | | |   | |  | |  || | | |  |  |  | || |    ||   | ||   || || |  |        ||| ||||||||| |||||||||||||||||||| |||||||||||| |  | || ||||| || |||||  ||||||| || 
gtatgtgaagtctttattcatgtttggtggttttaattgtctcttgctattaaattaatatgaacattataacttacaagtgcacattagattgttaacttcaaaatccctgcttgcagGCTTATGGTTCTGAAAATCAGCTCAAAGTTTGAACTACGTCGATTTTGCCGGACAACTGGTTCTGTTGCCATG

upper sequence: GRMZM2G381744_T01 (Zea mays), 3'ss of exon 6
lower sequence: GLYMA02G07910.1 (Glycine max), 3'ss of exon 6
---------------attattgtatgtatgttata-ttctccaatattggggactgtatgagtgtataggtgttgacctctttgttagcctttacttattgatgttctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT
               ||   ||| ||   ||| || || ||   || |   || |  ||  |   ||      ||  |  |   ||    | ||  |    |  | ||| ||  ||||| |||||||||||||||||||| |||||||||||| |  | || ||||| || || ||  ||||||| || 
gtatgtgaagtctttatccatgtttggtggttttaattgtctcttactattgaattaatatgaacattataactgcacattagattgttttctaacttcaaaattcctgcttttagGCTTATGGTTCTGAAAATCAGCTCAAAGTTTGAACTACGTCGATTTTGCCGGACAACCGGCTCTGTTGCCATG

upper sequence: GRMZM2G381744_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv08s0007g07460.t01 (Vitis vinifera), 3'ss of exon 6
attattgtatgtatgttatattctccaat--attggggactgtatgagtgtataggtgttg-acctctttgt-tagcctttacttattgatgttctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT
    ||| || | |  |  ||      ||  ||     |    |  | | |||   ||| | |   |  ||| |   ||  ||   ||  | |  || |||||| || |||||| ||||||||||||| |||||||||||  |  | || |||||||| ||||| ||||||||| ||
----ttgcatcttttcttcatcaagggatgcatatccaagaagaacaatctatgcatgtggtatggcgatgtatgaactgaaccagtttgtttcttgcttgcagACTTATGGTTTTGAAAATCAGCTCAAAGTTTGAACTGCGGCGATTTTGCCGTACAACTGGAGCTGTTGCAGTT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|28873655|gb|CB329613.1|CB329613
EST:     AGTGGAGCTGCAGTGGGTGACATGGCACTTCATTTCTGTGAACGTTATAA                         GCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCG
genomic: AGTGGAGCTGCAGTGGGTGACATGGCACTTCATTTCTGTGAACGTTATAAgtgagttttt ... ctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCG
EST: gi|9794809|gb|BE553117.1|BE553117
EST:     TCATTTCTGTGAACG-TATAA                         GCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCG
genomic: TCATTTCTGTGAACGTTATAAgtgagttttt ... ctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCG
EST: gi|60347495|gb|DN214468.1|DN214468
EST:     AGTGGAGCTGCAGTGGGTGACATGGCACTTCATTTCTGTGAACGTTATAA                         GCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCG
genomic: AGTGGAGCTGCAGTGGGTGACATGGCACTTCATTTCTGTGAACGTTATAAgtgagttttt ... ctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 413

TGGAGGAGCTTATCAAAGCTGTCGCTGACTCTGGTGCCAAGGTTATTGTCAGTGGAGCTGCAGTGGGTGACATGGCACTTCATTTCTGTGAACGTTATAAgtgagttttt ... ctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 188

TGGAGGAGCTTATCAAAGCTGTCGCTGACTCTGGTGCCAAGGTTATTGTCAGTGGAGCTGCAGTGGGTGACATGGCACTTCATTTCTGTGAACGTTATAAgtgagttttt ... ctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 197

TGGAGGAGCTTATCAAAGCTGTCGCTGACTCTGGTGCCAAGGTTATTGTCAGTGGAGCTGCAGTGGGTGACATGGCACTTCATTTCTGTGAACGTTATAAgtgagttttt ... ctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT


Block sizes: 46 (upstream exon), 47 (downstream exon)
Score: 203

TGGAGGAGCTTATCAAAGCTGTCGCTGACTCTGGTGCCAAGGTTATTGTCAGTGGAGCTGCAGTGGGTGACATGGCACTTCATTTCTGTGAACGTTATAAgtgagttttt ... ctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gtgtataggtgttgacctctttgttagcctttacttattgatgttctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT
                                   tattgat  putative branch site (score: 203)
                                           ttctgttt  CT-rich tract
                             tttacttatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
attattgtatgtatgttatattctccaatattggggactgtatgagtgtataggtgttgacctctttgttagcctttacttattgatgttctgtttgcagGCTGATGGTTCTGAAAATCAGCTCGAAGTTTGAACTAAGAAGGTTCTGCCGTACTACTGGGGCTGTTGCAATT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGTT
- - - - atgtatg
- - - - - - - - - - - - - - - - - - -ctgtatg
- - - - - - - - - - - - - - - - - - - - - - - - - - ggtgttg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgatgtt