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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtactaaacatattggatttctactgtccttgtcttgatgtgttttttttttgtcaatttgggatcttatatggaattaacccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAGGCCTTACCATCGGAGCTCTATCATTCGTCCTAGAACCAAGCCCGAAGAC

Basic information

species Zea mays
transcript GRMZM2G178859_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G178859_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os01g34390.1 (Oryza sativa), 3'ss of exon 6
gtaagtactaaacatattggatttctactgtccttgtcttgatgtgttttttttttgtcaatttgggatcttatatggaattaacccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAGGCCTTACCATCGGAGCTCTATCATTCGTCCTAGAACCAAGCCCGAAGAC
                |  |   |||  | | ||| |     |   || ||||| ||  ||      ||  | ||||  | |  |  ||  ||||||| |||||||| |||||||||||| ||||||| ||||| ||||| || |||||||| || |||||||||||||| || || || |||| ||| || ||
---------------gtaagtactcttatatacttttgcc--tcccttatttttctgaaaa---aaaatact-tatgagacttgcattt--tagGGGGTCTCACATCTGAGTGGTTCAGTCTTGAAGCATCACTTCTAACTTTGGGCCTTACAATTGGAGCTCTATCATTTGTGCTGGAGCCAACCCCCAAAAC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71318332|gb|DR795928.1|DR795928
EST:     GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
EST: gi|94479184|gb|EB708142.1|EB708142
EST:     TTGGGGTTATTTGCGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: TTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
EST: gi|211061642|gb|FL451761.1|FL451761
EST:     CATGCTACCTTTGGGGTAATTT-CGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: CATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
EST: gi|71443477|gb|DR824527.1|DR824527
EST:     GGGTTATTTGCGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: GGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
EST: gi|211061639|gb|FL451758.1|FL451758
EST:     GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
EST: gi|211061640|gb|FL451759.1|FL451759
EST:     GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
EST: gi|23199722|gb|BU582287.1|BU582287
EST:     GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
EST: gi|211061641|gb|FL451760.1|FL451760
EST:     GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: GCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
EST: gi|71322419|gb|DR797880.1|DR797880
EST:     AAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACT                         GGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG
genomic: AAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 48

CGAATGTTTTCTTTTGCTGACCCTACCGGTAGAAGAACTGCATGGGTGTCGCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAGGCCTTACCATCGGAGCTCTATCATTCGTCCTAGAACCAAGCCCGAAGAC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 82

CGAATGTTTTCTTTTGCTGACCCTACCGGTAGAAGAACTGCATGGGTGTCGCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAGGCCTTACCATCGGAGCTCTATCATTCGTCCTAGAACCAAGCCCGAAGAC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 103

CGAATGTTTTCTTTTGCTGACCCTACCGGTAGAAGAACTGCATGGGTGTCGCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAGGCCTTACCATCGGAGCTCTATCATTCGTCCTAGAACCAAGCCCGAAGAC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 61

CGAATGTTTTCTTTTGCTGACCCTACCGGTAGAAGAACTGCATGGGTGTCGCTAAGAAATTGTCTGTACATGCTACCTTTGGGGTTATTTGCGTACAACTgtaagtacta ... cccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAGGCCTTACCATCGGAGCTCTATCATTCGTCCTAGAACCAAGCCCGAAGAC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgatgtgttttttttttgtcaatttgggatcttatatggaattaacccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAGGCCTTACCATCGGAGCTCTATCATTCGTCCTAGAACCAAGCCCGAAGAC
                                       aattaac  putative branch site (score: 61)
                                             ccctt  CT-rich tract
       ttttttttttgtcaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtactaaacatattggatttctactgtccttgtcttgatgtgttttttttttgtcaatttgggatcttatatggaattaacccttggtagGGGGGCTCACATCCGAGTGGTTCAGTTTTGAAGCTTCACTCCTAACATTAGGCCTTACCATCGGAGCTCTATCATTCGTCCTAGAACCAAGCCCGAAGAC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CGAAGA