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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtacgtatttctatatgttttggcaattcttaacgatttattccatcgccttgtcttagttgtgggttgcccttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG

Basic information

species Zea mays
transcript GRMZM2G129513_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G129513_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os08g44810.1 (Oryza sativa), 3'ss of exon 6
gtacgta-tttctatatgttttggcaat-tcttaacgatttattccatcgccttgtcttagttgtgggttgccc---ttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG
||| | | ||| | |||||||| | | | | | ||||| | || | ||| | | || | || | | ||||| ||||||| || || ||||| || |||||||| |||||||||||||||||||||||||||||||||||||||||||| |||||||||
gtatggaatttttttatgttttctaagtgtgtcattattttatactattatcatgtttgaattaagttttttttaaacttttgtagTGCATTGATTTGCTTAAAGAATGCTCCCAACATACCAGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCCAAGTGCCAG

upper sequence: GRMZM2G129513_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv13s0019g05250.t01 (Vitis vinifera), 3'ss of exon 6
---------------------------------------------------gtacgtatttctatatgttttggcaat--tcttaacg---atttattccatcgccttgtctt----agttgtgggttgcccttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG
|| || | | | | | |||| || |||| | | | || | || |||| | ||||| | | ||||||| |||| ||| |||||||||| ||||||||||| ||||| ||||||||||| ||||| ||||| ||||| ||||||||||| || |||
gtaagaagcattgatttttctttcgttcattcttcttttgtgtgagcatgtgtgtgtgtgtgtgttttttttattgatgttcttggtgtgcagtcctttgtcctccctgtcatttatgattgtgtttcctttcctatgcagTGCATTGATATGTATGAAAAATGCTCCAAACATACCTGCAAAGAACTTTCATGCTTTGACTAGGTTAGATGAGAATAGAGCAAAATGTCAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211395431|gb|FL283721.1|FL283721
EST:     GCCTCGCGGAACGCGAAAGTCTTAGTTGTTGGAAATCCCTGTAACACTAA                         TGCGCTGATTTGTTTGAAAAATGCGCCAAACATACCGGCAAANAACTTTCA
genomic: GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
EST: gi|32916975|gb|CF021787.1|CF021787
EST:     GCCTCGCGGAACGCGAAAGTCTTAGTTGTTGGAAATCCCTGTAACACTAA                         TGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
genomic: GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
EST: gi|211398041|gb|FL283723.1|FL283723
EST:     GCCTCGCGGAACGCGAAAGTCTTAGTTGTTGGAAATCCCTGTAACACTAA                         TGCGCTGATTTGTTTGAAAAATGCGCCAAACATACCGGCAAAAAACTTTCA
genomic: GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
EST: gi|211398042|gb|FL283724.1|FL283724
EST:     GCCTCGCGGAACGCGAAAGTCTTAGTTGTTGGAAATCCCTGTAACACTAA                         TGCGCTGATTTGTTTGAAAAATGCGCCAAACATACCGGCAAAAAACTTTCA
genomic: GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
EST: gi|211395432|gb|FL283722.1|FL283722
EST:     GCCTCGCGGAACGCGAAAGTCTTAGTTGTTGGAAATCCCTGTAACACTAA                         TGCGCTGATTTGTTTGAAAAATGCGCCAAACATACCGGCAAAAAACTTTCA
genomic: GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
EST: gi|13558383|gb|BG549739.1|BG549739
EST:     GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAA                         TGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
genomic: GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
EST: gi|13490961|gb|BG517725.1|BG517725
EST:     GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAA                         TGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
genomic: GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
EST: gi|30705491|gb|CD058800.1|CD058800
EST:     GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAA                         TGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA
genomic: GCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 33

GGGAAAGCACTTAATGCGGTCGCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 840

GGGAAAGCACTTAATGCGGTCGCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG


Block sizes: 39 (upstream exon), 35 (downstream exon)
Score: 9

GGGAAAGCACTTAATGCGGTCGCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 608

GGGAAAGCACTTAATGCGGTCGCCTCGCGGAACGCGAAAGTCTTAGTTGTCGGAAATCCCTGTAACACTAAgtacgtattt ... cttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttcttaacgatttattccatcgccttgtcttagttgtgggttgcccttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG
                                        ttgccctt  CT-rich tract
 atttatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtacgtatttctatatgttttggcaattcttaacgatttattccatcgccttgtcttagttgtgggttgcccttgacgcagTGCGCTGATTTGTTTGAAAAATGCCCCAAACATACCGGCAAAAAACTTTCATGCATTGACGAGGTTGGATGAAAATAGAGCAAAGTGCCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAAG