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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttgttacaagccaatgagcccctcttttggcaaccgagtaagaattacagttgtttacaccagcagttgctgatcctcaagttctgattattttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT

Basic information

species Zea mays
transcript GRMZM2G121456_T02
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G121456_T03 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os03g19510.1 (Oryza sativa), 3'ss of exon 8
------ttgttacaagccaatgagcccctcttttggcaaccgagtaagaattacagttgtttacaccagcagttgctgatcctcaagttctgattattttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT
      ||   |||||| ||| |||  |||   ||||   | | |   | ||  |     | ||   |  | ||     ||    | ||   |||||||  || |||| |||||||||||||| |||||||| ||| | ||||||||||| ||||||||||||||||||||||| ||||||||||||||||||||||||||||| |
catttatttccacaagcgaat-agcaactccgctggctttctattgttacttgta-----tcaccagaagatttttctgtcactgattttccattatttcttttagGCTAATGAGATGCTGCATCACAAGGCTAACCTGAATGGATACCTAGCATACCACACTGGGCAGCCCCTAGATAAGATCAACGTAGATACTGACCGTGATT

upper sequence: GRMZM2G121456_T03 (Zea mays), 3'ss of exon 8
lower sequence: AT1G02560.1 (Arabidopsis thaliana), 3'ss of exon 8
-----------------ttgttacaagccaatgagcccc-----tcttttggcaaccgagtaagaattacagttgttt--acaccagcagttgctgatcctcaagttc------tgattattttat-tcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT
                 || |     |    | |  |||     |||| |      |  | |   | || | |  |||  | | |||  | |  |||  |  ||   |      | ||  | | |   ||||| ||||| |||||||| |||||||| ||| |||| || ||||| |||||||||||||| ||   |||||| ||||| |||   || || |||||||| |
gtcattcatcctcgcctttatatttcggtttttaatcccagtcatcttcttaagttcttggagcgactagattgatttgaatatcagatgattttgaaacctaaccacacaagttcatggtctgaaaccagGCAAATGAAATGCTGCATCACAAGGCAAACCTAAACGGTTACCTCGCATACCACACTGGTCAAAGCCTGGAGAAGATAAACCAGGACACAGACCGTGATT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|19769319|gb|BQ034040.1|BQ034040
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|149088220|gb|EE287341.2|EE287341
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|88752492|gb|DY536633.1|DY536633
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCCCAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|20208074|gb|BQ134163.1|BQ134163
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|32942454|gb|CF047273.1|CF047273
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|71307771|gb|DR790104.1|DR790104
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|76281084|gb|DV020652.1|DV020652
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|149043157|gb|EE047302.2|EE047302
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|74237087|gb|DT645001.1|DT645001
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|76012849|gb|DT940019.1|DT940019
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCCCAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
EST: gi|148934817|gb|EC878863.2|EC878863
EST:     ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAG                         GCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC
genomic: ATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 355

GAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 439

GAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 210

GAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 214

GAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGgtaaactcgc ... ttttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tacagttgtttacaccagcagttgctgatcctcaagttctgattattttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT
                                    ttctgat  putative branch site (score: 214)
                                             ttttattc  putative PPT
                                    ttctgattattttatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttgttacaagccaatgagcccctcttttggcaaccgagtaagaattacagttgtttacaccagcagttgctgatcctcaagttctgattattttattcagGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGATCA
- - - caagcca
- - - - - - - - - - - - - - - - - - - - - - - - -gttgttt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -caccagc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gttgctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttctgat