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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagcgttcttttctttatttggttcaaatatgttagtatctatgcaaagttaatcatttgtctcaacatgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG

Basic information

species Zea mays
transcript GRMZM2G095778_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G095778_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os02g53810.1 (Oryza sativa), 3'ss of exon 6
----------------------------gtaagcg--ttcttttcttta--tttggttcaaatatgttagtatctatgca---aagttaatcatttgtctcaacatgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
                             | |||   ||||| ||||||  | | ||||| |      | | |||    |   ||| |||||||||   | ||||  |||| |||||||||||||||||| ||| ||||||| ||||| |||
gtatgtattttagttttcttgtgctaatatcagcacattcttatctttagctgtagttcagaagatacaatctctcaatatgcaagctaatcattttctttaacactcttttcagTGCATTTGAGAAGTCCCTTTGCTTCCGATTGCAGAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|76015881|gb|DT943051.1|DT943051
EST:     GTTAGGCTAGTAGTAAGCTACAATGGTCTCAGCATTGGCACAGTTCGAGA                         TGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
genomic: GTTAGGCTAGTAGTAAGCTACAATGGTCTCAGCATTGGCACAGTTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
EST: gi|37377338|gb|CF625266.1|CF625266
EST:     GTTAGGCTAGTAGTAAGCTACAATGGTCTCAGCATGGGCACAGTTCGAGA                         TGCA-TTGAGAAAGTCTC-TGGCTTTCCGGTTGCAAAAG
genomic: GTTAGGCTAGTAGTAAGCTACAATGGTCTCAGCATTGGCACAGTTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAG-AAGTCTCTTGGC-TTCCGGTTGCAAAAG
EST: gi|211482002|gb|FL392969.1|FL392969
EST:     CTCAGCATTGGCACAGTTCGAGA                         TGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
genomic: CTCAGCATTGGCACAGTTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
EST: gi|211430121|gb|FL449154.1|FL449154
EST:     AATGGTCTCAGCATTGGCACAGTTCGAGA                         TGCATATGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
genomic: AATGGTCTCAGCATTGGCACAGTTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
EST: gi|89760691|gb|DY689000.1|DY689000
EST:     ATGGTCTCAGCATTGGCACAGTTTCGAGG                         TGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
genomic: ATGGTCTCAGCATTGGCACAG-TTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 37 (downstream exon)
Score: 51

GGAAAATATTGATATGACTGTTAGGCTAGTAGTAAGCTACAATGGTCTCAGCATTGGCACAGTTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG


Block sizes: 49 (upstream exon), 37 (downstream exon)
Score: 58

GGAAAATATTGATATGACTGTTAGGCTAGTAGTAAGCTACAATGGTCTCAGCATTGGCACAGTTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG


Block sizes: 44 (upstream exon), 37 (downstream exon)
Score: 49

GGAAAATATTGATATGACTGTTAGGCTAGTAGTAAGCTACAATGGTCTCAGCATTGGCACAGTTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG


Block sizes: 46 (upstream exon), 37 (downstream exon)
Score: 43

GGAAAATATTGATATGACTGTTAGGCTAGTAGTAAGCTACAATGGTCTCAGCATTGGCACAGTTCGAGAgtaagcgttc ... tgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tcaaatatgttagtatctatgcaaagttaatcatttgtctcaacatgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG
                        agttaat  putative branch site (score: 43)
                                             tgctttcc  putative PPT
                      aaagttaatcattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagcgttcttttctttatttggttcaaatatgttagtatctatgcaaagttaatcatttgtctcaacatgctttccagTGCATTTGAGAAGTCTCTTGGCTTCCGGTTGCAAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGT