ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtttaaaaccttatctagcttaattgtgattgccttgattatgccattatagaacttaatatttggatgtattatgcgactaagacatattacctttcttctctgttgaaaagGCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCATGGTTTTGTTCCTCCATTCTTGGCACGTCCTTATCTGCAAACAAATGTC

Basic information

species	Zea mays
transcript	GRMZM2G080930_T03
intron #	6
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G080930_T03 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os02g54770.1 (Oryza sativa), 3'ss of exon 6

gtttaaaaccttatctagcttaat-tgtgattgccttgattatgccattatagaacttaatatttggatgtattatgcgactaagacatattacctttcttctctgt--tgaaaagGCAT-GTTCTGCTTAAACGACTCGGCCAACTAC-GGCTCTCAGTTAGTCTTCATGGTTTTGTTC------CTCCATTCTTGGCACGTCCTTATCTGCAAACAAATGTC
|||||  || ||||||  ||| || | | |    |||  || || | ||| | ||||||     |||||||| ||||| ||||||||||||||||||||| || |||  | || ||| ||  |||||||||| |||||| ||||||||| ||||||      |||| |||||||||||||      || ||||| | |  |||  ||  ||||   ||  ||  
gtttactactttatctgccttgatatttcagcctcttatttctgacgttaaaaaacttatgggatggatgtaatatgcaactaagacatattacctttctactatgtgctaaagAGGGATCATTCTGCTTAAGCGACTCAGCCAACTACCGGCTCT------GTCTGCATGGTTTTGTTCTGCACTCTTCATTCCTCGAGCGTG-TTGCCTGCTT-CATGTG--

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|93284594|gb|EB676858.1|EB676858

EST:     CAACTGCATGACTTCAGTTCAG                         GCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA
genomic: CAACTGCATGACTTCAGTTCAGgtttaaaacc ... tgttgaaaagGCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA

EST: gi|6102005|gb|AW126475.1|AW126475

EST:     CATGACTTCAGTTCAG                         GCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA
genomic: CATGACTTCAGTTCAGgtttaaaacc ... tgttgaaaagGCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA

EST: gi|60342272|gb|DN209245.1|DN209245

EST:     TCAGGAACAAATTTGGAACTGTCGTCAACTGCATGACTTCAGTTCAG                         GCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA
genomic: TCAGGAACAAATTTGGAACTGTCGTCAACTGCATGACTTCAGTTCAGgtttaaaacc ... tgttgaaaagGCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA

EST: gi|67013664|gb|CO442413.1|CO442413

EST:     CTGTCAGGAACAAATTTGGAACTGTCGTCAACTGCATGACTTCAGTTCAG                         GCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA
genomic: CTGTCAGGAACAAATTTGGAACTGTCGTCAACTGCATGACTTCAGTTCAGgtttaaaacc ... tgttgaaaagGCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA

EST: gi|76911951|gb|DV164503.1|DV164503

EST:     CTGTCAGGAACAAATTTGGAACTGTCGTCAACTGCATGACTTCAGTTCAG                         GCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA
genomic: CTGTCAGGAACAAATTTGGAACTGTCGTCAACTGCATGACTTCAGTTCAGgtttaaaacc ... tgttgaaaagGCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 39 (downstream exon)
Score: 10

GAAGCCCGTGCCTGATACTTCTTGGTGTGAGAACTTAGTCCTTGGTTTGGCTGTCAGGAACAAATTTGGAACTGTCGTCAACTGCATGACTTCAGTTCAGgtttaaaacc ... tgttgaaaagGCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCATGGTTTTGTTCCTCCATTCTTGGCACGTCCTTATCTGCAAACAAATGTC

Block sizes: 20 (upstream exon), 30 (downstream exon)
Score: 2

Block sizes: 49 (upstream exon), 40 (downstream exon)
Score: 5

Block sizes: 46 (upstream exon), 42 (downstream exon)
Score: 6

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

atatttggatgtattatgcgactaagacatattacctttcttctctgttgaaaagGCATGTTCTGCTTAAACGACTCGGCCAACTACGGCTCTCAGTTAGTCTTCATGGTTTTGTTCCTCCATTCTTGGCACGTCCTTATCTGCAAACAAATGTC