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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgtttatctaacatagtattaccaagagatgcatgcttgtcttggtttgtatttactctcagcaactttagtttctaagacattcattcatgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG

Basic information

species Zea mays
transcript GRMZM2G078756_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G078756_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os12g34860.1 (Oryza sativa), 3'ss of exon 6
tgtttatctaacatagtattaccaagagatgcatgcttgtcttggtttgtatttactctcagcaactttagtttctaagacattcat--tcatgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
|||    |   ||| | |||  |        | |  | |    | | ||  | |  |   | ||| |||| |  |||| |  | |||  |  |      ||||| ||||||||||| |||||||||||||| |||||||| ||||||
tgtcctgccttcattgcatttactcttataactttatggctaagatgtgagtattgttata-caatttta-tggctaaaatgtgcatattattatctttcagTTTTCAGAAGGCAAACTTGGTGTCAAGTTTAAGCCATTCTCAAAG

upper sequence: GRMZM2G078756_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv11s0103g00120.t01 (Vitis vinifera), 3'ss of exon 6
-------------------------------------------------------------------------tgtttatctaacatagtattaccaagagat----gcatgcttgtcttggtttgtatttac--tctcagcaact---ttagtttctaaga--------cattcattcatgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
                                                                         |  ||  | ||  |   ||      || |     |||||||   | ||     | || ||  ||||  || ||   |||||| |   ||         ||  || | |  ||  ||||||||| | ||| |||| || ||||||||||||||||||||||||
gtaattccccaattcagtaaatgatactgtattttattttattctccttttctatgtctttgtggttaagtaatcatttccaaaattgaaatccttgggaaaataaagcatgctgaacatgaaccttgttgacattctcgacatctgatttagttcccctgaatgtttaatataaatgctttgtatgcagTTCTCAAAGGGCCAGCTAGGGGTCAAGTTCAAGCCATTTTCAAAG

upper sequence: GRMZM2G078756_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv19s0027g01200.t01 (Vitis vinifera), 3'ss of exon 6
-----------------------------------------------------------------------tgtttatctaacatagtattaccaagagat----gcatgct------tgtcttggtttgtatttactctcagcaactttagtttctaagacattcattc---atgct----acccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
                                                                       |  ||| | ||  |  |||    | || |     |||||||      || | | |||   |||     |     | ||  ||| |    |  || | |    |||||    |  ||||||||| | | | ||||||| ||||||||||||||||||||||||
gtaattccccaattcagtaaatgatactgtattttattttattctccttttctgtctttgtggttaagtaatcattaccaaaattgatatccttaggaaaataaagcatgctgaaaattgaccttgttgacattcttaatgtctgatttaggttccctgaatgtttaatttaaatgctttgtatgcagTTCTCAAAGGACCAGCTTGGGGTCAAGTTCAAGCCATTTTCAAAG

upper sequence: GRMZM2G078756_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv19s0027g01480.t01 (Vitis vinifera), 3'ss of exon 1
-------------------------------------------------------------------------tgtttatctaacatagtattaccaagagat----gcatgct------tgtcttggtttgtatttactctcagcaactttagtttctaagacattca-ttcatgc------tacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
                                                                         |  ||  | ||  |  |||    | || |     |||||||      || | | |||   |||     |     | ||  ||| |    |  || | || |  |      ||  ||||||||| | ||| ||||||| ||||||||||||||||||||||||
gtaattccccaattcagtaaatgatattctattttattttattctccttttctttgtctttgtggttaagtaatcatttccaaaattgatatccttaggaaaataaagcatgctgaaaattgaccttgttgacattcttaatgtctgatttgggttccctgaatgtttaatttaaacactttgtatgcagTTCTCAAAGGGCCAGCTTGGGGTCAAGTTCAAGCCATTTTCAAAG

upper sequence: GRMZM2G078756_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv19s0027g01150.t01 (Vitis vinifera), 3'ss of exon 7
-------------------------------------------------------------------------tgtttatctaacatagtattaccaagagat----gcatgct------tgtcttggtttgtatttactctcagcaactttagtttctaagacattca-ttcatgc------tacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
                                                                         |  ||  | ||  |  |||    | || |     |||||||      || | | |||   |||     |     | ||  ||| |    |  || | || |  |      ||  ||||||||| | ||| ||||||| ||||||||||||||||||||||||
gtaattccctaattcagtaaatgatactgtattttattttattctccttttctatgtctttgtggttaagcaatcatttccaaaattgatatccttaggaaaataaagcatgctgaaaattgaccttgttgacattcttaatgtctgatttaggttccctgaatgtttaatttaaacactttgtatgcagTTCTCAAAGGGCCAGCTTGGGGTCAAGTTCAAGCCATTTTCAAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211308521|gb|FL467499.1|FL467499
EST:     TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAG                         TTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
genomic: TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
EST: gi|76910024|gb|DV163657.1|DV163657
EST:     TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAG                         TTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
genomic: TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
EST: gi|31072245|gb|BQ479053.1|BQ479053
EST:     TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAG                         TTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
genomic: TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
EST: gi|71762613|gb|DR960550.1|DR960550
EST:     TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAG                         TTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
genomic: TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
EST: gi|78079223|gb|DV507635.1|DV507635
EST:     AACGGTTCACGTCCCAG                         TTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
genomic: AACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
EST: gi|71310636|gb|DR791614.1|DR791614
EST:     TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAG                         TTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
genomic: TTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
EST: gi|211026939|gb|FL261356.1|FL261356
EST:     ATAAACGGTTCACGTCCCAG                         TTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
genomic: ATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 112

ATGGGCCTTTGGATTGGGATTGGAGCGTCTTGCGATGGTCCTTTTCGACATTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 125

ATGGGCCTTTGGATTGGGATTGGAGCGTCTTGCGATGGTCCTTTTCGACATTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG


Block sizes: 48 (upstream exon), 45 (downstream exon)
Score: 63

ATGGGCCTTTGGATTGGGATTGGAGCGTCTTGCGATGGTCCTTTTCGACATTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG


Block sizes: 43 (upstream exon), 45 (downstream exon)
Score: 69

ATGGGCCTTTGGATTGGGATTGGAGCGTCTTGCGATGGTCCTTTTCGACATTCCAGATATTCGACTATTCTGGTCGAATGATAAACGGTTCACGTCCCAGgtactgctac ... tgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttgtatttactctcagcaactttagtttctaagacattcattcatgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG
                     tttagttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgtttatctaacatagtattaccaagagatgcatgcttgtcttggtttgtatttactctcagcaactttagtttctaagacattcattcatgctacccagTTCTCAGAAGGCAAGCTTGGTGTCAAGTTCAAGCCATTTTCAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAAGCT
- - - - - - - - - - - - - - -tgcatgc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -acattca