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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...agttcttattcttgtggcaaacagcacacgcataatgctttgcaatcctcatacatatcgccagcctaattttaccattttatcatcaaaccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG

Basic information

species Zea mays
transcript GRMZM2G069758_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G069758_T04 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os12g40830.3 (Oryza sativa), 3'ss of exon 6
agttcttattcttgtggcaaacagcacacgcataatgctttgca-atcctcatacat-atcgcc-agcctaattttaccattttatcatcaaaccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG
  || ||  || ||   |  | ||||  | |  | |  |   ||   || |||| || ||  |  | ||||||     |||||| | ||| || |||   |||||||| ||||||||| ||||||||||||||||||||||||||||||||||||| ||||| || ||
--ttatttctcatgattctta-agcatgctctaattcttgcacatgcccccatatatgattacttatcctaatacattcattttgtgatctaatcatccttagGTTGTCCCAGTTGCTACAATGCTTACACCTAACCAATTTGAAGTTGAACTACTCACTGGTTTAAG

upper sequence: GRMZM2G069758_T04 (Zea mays), 3'ss of exon 6
lower sequence: Vv14s0006g01660.t01 (Vitis vinifera), 3'ss of exon 6
agttcttattcttg--tggcaaacagcacacgcataatgctttgcaatcctcatacatatcgccagc--ctaattttaccattttatcatcaaaccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG
   |   ||   |   || ||   ||      ||| |   ||  | |||   ||   |||  || |   | ||  | || |  |   | |      ||   |||||||||||||||||||||||| | || ||||| || |||||||  |||||||| ||||| || ||
-acttgaataaataattgccattgagtttgatcatgaattttgtctatctgtattattatttcctgatgcaaagatcactagctagacttt---ttatggttagGTTGTTCCAGTTGCTTCAATGTTGACTCCTAATCAGTTTGAAGCAGAACTACTAACTGGGTTCAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|33097992|gb|CF057952.1|CF057952
EST:     GCTAATATCTGTTTATCAACAGAAG                         GTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
genomic: GCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
EST: gi|211278750|gb|FL388232.1|FL388232
EST:     AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAG                         GTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
genomic: AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
EST: gi|86471052|gb|DY237422.1|DY237422
EST:     AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAG                         GTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
genomic: AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
EST: gi|33096068|gb|CF056062.1|CF056062
EST:     AAGGAAAACTATATGTTCCTCAGGGGCTAATATCTGTTTATCAACAGAAG                         GTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
genomic: AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
EST: gi|17931888|gb|BM268848.1|BM268848
EST:     AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAG                         GTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
genomic: AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
EST: gi|67022839|gb|CO451588.1|CO451588
EST:     AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAG                         GTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
genomic: AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
EST: gi|40303606|gb|CK347993.1|CK347993
EST:     AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAG                         GTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
genomic: AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
EST: gi|16922789|gb|BM075781.1|BM075781
EST:     AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAG                         GTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA
genomic: AAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 62

TTTGTGACCCAGTTCTAGGTGATGAAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 205

TTTGTGACCCAGTTCTAGGTGATGAAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 292

TTTGTGACCCAGTTCTAGGTGATGAAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 154

TTTGTGACCCAGTTCTAGGTGATGAAGGAAAACTATATGTTCCTCAGGAGCTAATATCTGTTTATCAACAGAAGgtgatttagc ... ccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tcctcatacatatcgccagcctaattttaccattttatcatcaaaccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG
                  gcctaat  putative branch site (score: 154)
                     taattttaccatttta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agttcttattcttgtggcaaacagcacacgcataatgctttgcaatcctcatacatatcgccagcctaattttaccattttatcatcaaaccatttgtagGTTGTTCCAGTTGCTTCAATGCTTACACCTAACCAATTTGAAGTTGAACTACTTACTGGATTGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - -attcttg
- - - - - - - - gcaaaca
- - - - - - - - - - - - cacacgc
- - - - - - - - - - - - - - - - - - -ctttgca
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - cgccagc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -catcaaa