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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgaatatgacaataatgaattatcattacaccaatttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT

Basic information

species Zea mays
transcript GRMZM2G014805_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os12g30540.1 (Oryza sativa), 3'ss of exon 6
gtgaatatgacaataatgaattatcattacaccaatttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
|||||||| | || ||  | ||     ||   | |   | || ||||||||||| | ||  || |||| ||||||||||||||||||||||||||||||||| |||||||||||||| || || || ||||||||||| || || ||||||||||||||||||||||
gtgaatattataacaaacattttattatatgtctacg-atttgttattaactttgcgttg-acccagGAAACTGTTGTGGAAGGAACAATTGAACAATTATTTGAAGGCCACCACATAAACTATATCGAGTGCATTAATGTTGATTATAAATCTAACAGAAAAGAAT

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 6
lower sequence: GLYMA13G16170.1 (Glycine max), 3'ss of exon 6
-----------------gtgaatatgacaataatgaattatcattac--acca-atttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
                 |   || ||   || || | |  | ||     |||| ||  | |  || ||   | || ||| |   |||||||||||||| || ||||| ||  || ||||||| ||||| ||||| || |||||||| || ||||| || || ||||| ||||| |  ||||| || |
gtatggcaagagttttggaatatttgttcatgattattcttgatgggtgaccatatcaaatggttgttttgtgttatttgtcttcagGGAACTGTTGTTGAGGGAACCATACAAAAATTATTTGAAGGGCACCATATGAATTACATAGAATGCATCAATGTAGACTACAAATCAACTAGAAAGGAGT

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 6
lower sequence: GLYMA10G23680.1 (Glycine max), 3'ss of exon 2
---------------------------------------------gtgaatat-gacaataatgaattatcattacacc--aatttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
                                              | | | | || | |  || ||| | || |   |   |   | | ||| ||    |||| |  |   |||||||||||||||||||| || ||  | ||||| || ||||| ||||| || || || ||||| ||||| || ||||| |||||||| |  || |||||||
gtactggtttttcatgatggctcttattgtcttcttaaaatagcttttactctagatacttgtgtatttttatgatgtcttgaaagattatttgttggggtttcatcttggccagGGAACTGTTGTGGAAGGCACTATACAGCAATTGTTTGAAGGACACCATATGAACTATATTGAATGCATAAATGTGGATTATAAATCAACAAGGAAAGAAT

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 6
lower sequence: AT3G11910.2 (Arabidopsis thaliana), 3'ss of exon 6
gtgaata----tgacaataatgaattatcatt-acaccaatttactttttat----taacttttctttctacaca-gGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
|| |||     |    ||  ||   | || || |  |  | ||  | || ||    ||| |||| |   |   || ||||||||||||||| ||||||||  || |  |||| ||||| |||||||| |||||||||||||||||||| || || || |||||||   | ||||| |
gtaaattgtcttcttcatcgtgttctttctttcattctcagttggtgttcatgttttaatttttttcctttttcaagGGAACTGTTGTGGAGGGAACAATACAAAAGCTATTTGAAGGTCACCACATGAATTACATTGAGTGCATTAATGTAGATTACAAATCTACACGGAAAGAGT

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 6
lower sequence: AT5G06600.1 (Arabidopsis thaliana), 3'ss of exon 6
-----------gtgaatatgacaataatgaattatcattacaccaat--ttactttttattaacttttctttctacac--------agGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
            |   | | |     |||| |||  | |       |  ||  | ||| | | || || ||||| |          |||||||||||||||| ||||||||  ||||| | || || |||||||| || ||||||||||||||||| || || || | ||||||||   | |||||||
gtaatttttcttttgttgtca-ggccatgatttactactcagttggtgtttcttgtttttgagctcttttttcttcgtttttctgtagGGAACTGTTGTGGAGGGAACAATACAACAACTTTTTGAGGGCCACCATATGAATTACATTGAGTGCATAAATGTAGATTTTAAATCTACACGGAAAGAAT

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv13s0073g00610.t01 (Vitis vinifera), 3'ss of exon 6
---------------------------gtgaatatgacaataatgaattatcattacaccaatttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
                             || |       | || |   |   |   |     |||  ||| |||    ||| |     | ||||||||||||||||| || || ||  | || ||||| ||||| ||||| || || || ||||| |||||||| |||||||||||||| |  ||||| ||||
gtataggcgcttaacattgccacttgttggacttcagtgcttataagcaagtttgggaaatcattaaatttgattgttatttttaatggctcagGGAACTGTTGTGGAGGGTACTATACAGCAGTTATTTGAAGGGCACCATATGAACTATATTGAATGCATTAATGTGGACTATAAATCAACAAGAAAGGAAT

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv08s0032g00600.t01 (Vitis vinifera), 3'ss of exon 6
----gtgaa-tatgacaataatgaattatcat----tacaccaatttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
     |||  | | |||        ||    |    | |||   | |    ||| |||  || | || ||  |||||||||||| ||||| || |||||  | || || || ||||| ||||| || || |||||||||||||| || |||||||| |||||||  ||||| ||||
gtatatgaggtttaacagctgcagctttcagtatttttcactgttctgaagttttttattttatattactggacagGGAACTGTAGTGGAGGGGACAATACAGCAGTTGTTTGAAGGGCACCATATGAACTACATTGAGTGCATCAATGTGGACTACAAATCTACAAGAAAGGAAT

upper sequence: GRMZM2G014805_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv06s0009g00960.t01 (Vitis vinifera), 3'ss of exon 6
gtgaatatgacaataatgaattatcattacaccaatttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
     ||  |  || |    || |  |   |  | || | || | |  |   ||   | ||   ||||||||||||||||| || || ||  | || ||||| ||||| ||||| || || |||||||| ||||| || |||||||| |||||||  || || || |
----gtacaaacatgactg-ttgttgtggaattatttcagttctgacaattattaagtgcttgccagGGAACTGTTGTGGAGGGCACTATACAGCAGTTATTTGAAGGGCACCATATGAACTACATTGAATGCATCAATGTGGACTACAAATCTACAAGGAAGGAGT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|101389686|gb|EB816777.1|EB816777
EST:     TGCAAGAACTCAACAGAGTTCTTTGTGAGAAACTGGAAGATAAGATGAAG                         GGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATT
genomic: TGCAAGAACTCAACAGAGTTCTTTGTGAGAAACTGGAAGATAAGATGAAGgtgaatatga ... ttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATT
EST: gi|76920464|gb|DV168186.1|DV168186
EST:     TGCAAGAACTCAACAGAGTTCTTTGTGAGAAACTGGAAGATAAGATGAAG                         GGAACTGTTGTGGAAGGAACAATTGGACAATTATTCGAAGGCCACCACATT
genomic: TGCAAGAACTCAACAGAGTTCTTTGTGAGAAACTGGAAGATAAGATGAAGgtgaatatga ... ttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 495

GACTAAATCCTTTGGATGGGATACCTATGATTCTTTCATGCAGCATGATGTGCAAGAACTCAACAGAGTTCTTTGTGAGAAACTGGAAGATAAGATGAAGgtgaatatga ... ttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 372

GACTAAATCCTTTGGATGGGATACCTATGATTCTTTCATGCAGCATGATGTGCAAGAACTCAACAGAGTTCTTTGTGAGAAACTGGAAGATAAGATGAAGgtgaatatga ... ttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 285

GACTAAATCCTTTGGATGGGATACCTATGATTCTTTCATGCAGCATGATGTGCAAGAACTCAACAGAGTTCTTTGTGAGAAACTGGAAGATAAGATGAAGgtgaatatga ... ttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 488

GACTAAATCCTTTGGATGGGATACCTATGATTCTTTCATGCAGCATGATGTGCAAGAACTCAACAGAGTTCTTTGTGAGAAACTGGAAGATAAGATGAAGgtgaatatga ... ttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ataatgaattatcattacaccaatttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT
                                tattaac  putative branch site (score: 488)
                           ctttttattaactttt  CT-rich tract
                     aatttactttttatta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgaatatgacaataatgaattatcattacaccaatttactttttattaacttttctttctacacagGGAACTGTTGTGGAAGGAACAATTGAACAATTATTCGAAGGCCACCACATTAATTACATTGAGTGCATTAACGTGGACTATAAATCTAACAGAAAAGAAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA