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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgggcacggttgaccgccgaagtagctacttcgaaaattgcaagcgttagtcatttcgacttcaatggatgattttgtttgagaatttatttaattgttctacaaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAGAGACACAACAGTGCACAATTAACAAGATGACACATCCTTCCTAGTGATG

Basic information

species Zea mays
transcript GRMZM2G011404_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G011404_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os11g09170.1 (Oryza sativa), 3'ss of exon 6
gtgggcacggttgaccgccgaagtagctacttcgaaaattgcaagcgttagtcatttcgacttcaatggatgattttgtttgagaatttatttaattgttctacaaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAGAGACACAACAGTGCACAATTAACAAGATGACACATCCTTCCTAGTGATG
||||| || |||||||  | |  |||||| || ||     |||| | | | | | |   | ||  |||                            | ||||       |||||||||||||||| || |||||||| ||| |||||||||| ||  |||||||||||| || |||| ||||| ||||||||||||| |   |   | |  |||
gtgggtacagttgaccatctagctagctagtttga---ccgcaaccat-actaaatctaatttacatg---------------------------ctattct-----cgttcagGATTGTGAGAGGCGGTCAATAGCAAAGTTATTCCATCATCGATCAGAAGAGAGACGCAGCAGTCCACAACTAACAAGATGACAGAGATTCTTTCGGAATG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|37379704|gb|CF626601.1|CF626601
EST:     AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAG                         GATTGTGATAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
genomic: AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
EST: gi|87154167|gb|DY398956.1|DY398956
EST:     AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAG                         GATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
genomic: AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
EST: gi|7119098|gb|AW497478.1|AW497478
EST:     AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAG                         GATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
genomic: AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
EST: gi|211160450|gb|FL446939.1|FL446939
EST:     AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAG                         GATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
genomic: AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
EST: gi|40334682|gb|CK368752.1|CK368752
EST:     AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAG                         GATTGTGATAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG
genomic: AACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 23

TGATCGATTCACACCTCGTGACCAGCTCAGCTTTGCATATACATACTTAAAACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAGAGACACAACAGTGCACAATTAACAAGATGACACATCCTTCCTAGTGATG


Block sizes: 41 (upstream exon), 49 (downstream exon)
Score: 11

TGATCGATTCACACCTCGTGACCAGCTCAGCTTTGCATATACATACTTAAAACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAGAGACACAACAGTGCACAATTAACAAGATGACACATCCTTCCTAGTGATG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 16

TGATCGATTCACACCTCGTGACCAGCTCAGCTTTGCATATACATACTTAAAACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAGAGACACAACAGTGCACAATTAACAAGATGACACATCCTTCCTAGTGATG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 12

TGATCGATTCACACCTCGTGACCAGCTCAGCTTTGCATATACATACTTAAAACTGAGAAGGACAAATCCTGACAGACCATTCCGTCTCAATATGTTTAAGgtgggcacgg ... aaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAGAGACACAACAGTGCACAATTAACAAGATGACACATCCTTCCTAGTGATG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

acttcaatggatgattttgtttgagaatttatttaattgttctacaaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAGAGACACAACAGTGCACAATTAACAAGATGACACATCCTTCCTAGTGATG
                              atttaat  putative branch site (score: 12)
                                                ttttc  putative PPT
                       agaatttatttaattg  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgggcacggttgaccgccgaagtagctacttcgaaaattgcaagcgttagtcatttcgacttcaatggatgattttgtttgagaatttatttaattgttctacaaattttcagGATTGTGAGAGACGATCAATAGCGAAGCTATTCCATCACCGGACAGAAGAGAGACACAACAGTGCACAATTAACAAGATGACACATCCTTCCTAGTGATG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGATG