Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   		4  
 5'  3'   		5  
 5'  3'   		6  
 5'  3'   		7  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...agctttagctctttccttctgttggtgcacatgcatgctttatgtatatttgtttaattagttaatagttacaacgtacattcattctctaccatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATTTCGATAAGGAAACAAAAGAGTATGTGTCTACTTTAT

Basic information

species Zea mays
transcript GRMZM5G862959_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G862959_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os12g19350.1 (Oryza sativa), 3'ss of exon 4
agctttagctctt-tccttctgttggtgcacatgcatgctttatgtatatttgtttaattagttaatagttacaacgt---acattcattctctaccatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATTTCGATAAGGAAACAAAAGAGTATGTGTCTACTTTAT
  | | | |  || | | |   |  || |   ||| |  |||    || |  | | |  |  |  |||  |  || ||   |||||  | || | |||||||||||||| |||| ||| |||||||| || ||||||||||| |||||||| |||||||| ||||||||||||||||||||||||||                 
cacatcatcaattgtgcataaatcagttc---tgc-tcttttgcacatgtgcgataacatgctgtatatattaaatgtctcacatttgtgctatgccatttcagGTTGCAGCTAAGGTCAAATCAGACGAAGAAAAAGATGAATGGTTTGTGGTGAAGGTGATACATTTCGATAAGGAAACAAAAGA-----------------
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148974314|gb|EE025087.2|EE025087
EST:     CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAG                         GTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG
genomic: CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG
EST: gi|29944945|gb|CB815389.1|CB815389
EST:     CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAG                         GTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG
genomic: CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG
EST: gi|31358058|gb|CD442415.1|CD442415
EST:     CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAG                         GTTGCTGCTAGGGTAAAATCAGATGACGAAAAAG
genomic: CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAG
EST: gi|211487166|gb|FL254349.1|FL254349
EST:     CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAG                         GTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG
genomic: CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG
EST: gi|28873324|gb|CB329328.1|CB329328
EST:     CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAG                         GTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG
genomic: CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG
EST: gi|93012241|gb|EB637761.1|EB637761
EST:     CATCTATGACGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAG                         GTTGCTGCTAGGGTAAAATCACATGACGAAAAAGATGAGTGGTTTGCCGCG
genomic: CATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 116

GAAAAAGAAGAGGATCAAGTCTGATACAGATTCTGCACGATTTTCTGCTGCATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATTTCGATAAGGAAACAAAAGAGTATGTGTCTACTTTAT


Block sizes: 43 (upstream exon), 49 (downstream exon)
Score: 146

GAAAAAGAAGAGGATCAAGTCTGATACAGATTCTGCACGATTTTCTGCTGCATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATTTCGATAAGGAAACAAAAGAGTATGTGTCTACTTTAT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 161

GAAAAAGAAGAGGATCAAGTCTGATACAGATTCTGCACGATTTTCTGCTGCATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATTTCGATAAGGAAACAAAAGAGTATGTGTCTACTTTAT


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 96

GAAAAAGAAGAGGATCAAGTCTGATACAGATTCTGCACGATTTTCTGCTGCATCTATGAGGAATCAACTTGACCAAGCTGCAAGCCTTAAAGGAGAGCAGgttcaacttc ... accatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATTTCGATAAGGAAACAAAAGAGTATGTGTCTACTTTAT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atatttgtttaattagttaatagttacaacgtacattcattctctaccatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATTTCGATAAGGAAACAAAAGAGTATGTGTCTACTTTAT
              agttaat  putative branch site (score: 96)
                                       ttctctaccatttc  putative PPT
       tttaattagttaatag  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agctttagctctttccttctgttggtgcacatgcatgctttatgtatatttgtttaattagttaatagttacaacgtacattcattctctaccatttcagGTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATTTCGATAAGGAAACAAAAGAGTATGTGTCTACTTTAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAA