ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtaagaataaacaaaagcaacattttctcatgtgctgaatatgctttaggtaagcaactgacatatggtttcttttgctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG

Basic information

species	Zea mays
transcript	GRMZM5G862107_T01
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM5G862107_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os03g20100.1 (Oryza sativa), 3'ss of exon 5

gtaagaataaacaaaagcaacattttctcatgtgctgaatatgctttaggtaagcaactgacatatggtttcttttg-ctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG
|||||  | ||    ||||| |||   | || || |  || ||   |   ||   ||||||||||  ||||  | || ||||||  |||||||||||||||||||||||||||||||||| |||||||| |||||||| |||||||||||||| ||||| ||||||||||| |||
gtaagtttgaa----agcaatattccttgatctggtccatctggaatgtataca-aactgacatactgttttctctgtctcaaaacagGCTGATGAGATGGCTCAGATATTCAGGCAGAGGATAGCTCAAGCAGAGGCAATGGCTCGTGCTGATATGTTAAGATTCCAGCCTGAG

upper sequence: GRMZM5G862107_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA17G04190.1 (Glycine max), 3'ss of exon 5

----------gtaagaataaacaaaagcaacattttctcat--gtgctgaatatgctttaggtaagca-actgacatatggtttcttttgctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG
           |      || | ||  ||||   ||    |  |   || |  |  |  |||  |    ||| |||||    ||   ||  |     ||||| || ||||||||||||| |||||| ||||||||||| || ||||| |||||||||||||| |||||| | || |||||||| |||
catcacttacatgttttaaatctaattcaacccatttctgttagcattggaatttttgaaggagaatttactcacataatccttaacttttttgttatagGCGGAGGAGATGGCTCAGACATTCAGACAGAGAATAGCCCAAGCAGAAGCTATGGCTCGTGCAGACATGCTAAGGTTCCAGCCTGAG

upper sequence: GRMZM5G862107_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA15G14550.1 (Glycine max), 3'ss of exon 5

---------gtaagaataaacaaaagcaacattttctcatgtgctgaatatgctttaggtaagcaactg---acatatggtttct--tttgctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG
          |||  ||  ||   | |    ||   |    |  | | |||  |||  | |||  | ||   || |||  ||  |  | || |   |  |||| || ||||||||||||| |||||| |||||||| ||||| ||||| ||||||||||||||||| ||| | |||||||||||||||
tctatgtttttaatcatctacttcaacccttttcattagcattatcattatcatttttgaaagagaatgcacacttataattcttaatctgttgttaa-agGCGGAGGAGATGGCTCAGACATTCAGACAGAGAATTGCTCAAGCAGAAGCTATGGCTCGTGCTGATATGCTTAGATTCCAGCCAGAG

upper sequence: GRMZM5G862107_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA09G03660.1 (Glycine max), 3'ss of exon 5

---------gtaagaataaacaaaagcaacattttctcatgtgctgaatatgctttaggtaagcaactgacatatggtttcttttgctcaaat----tagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG
          |||  ||  ||   | |    |||  |    |  | | ||   |||  | |||  | ||     |  | | | ||  ||   |    ||||| || ||||||||||||| |||||| |||||||| ||||| ||||| ||||||||||||||||| ||| | |||||||||||||||
tctatgtttttaatcatctacttcaaccctttttgttatcattatcattaccatttttgaaagagaatgcacagttatatttcttaatctgttgttatagGCGGAGGAGATGGCTCAGACATTCAGACAGAGAATTGCTCAAGCAGAAGCTATGGCTCGTGCTGATATGCTTAGATTCCAGCCAGAG

upper sequence: GRMZM5G862107_T01 (Zea mays), 3'ss of exon 5
lower sequence: AT5G30510.1 (Arabidopsis thaliana), 3'ss of exon 5

gtaagaataaacaaaagcaacattttctcatgtgctgaatatgctttaggtaagcaactgacatatggtttcttttgctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG
         |||   |    ||||     |     |   |||  ||    || |   |   ||| || |   || ||||      ||||||| ||||||||||||| |||||| |||||||| || || ||||| || ||||||||||| |||||| |  | ||||| || |||
-------gtaactctatttccattcaaaaaaactttccctat-tttcttttatgtctcaatcatttgctactttctgctgtctcaagGCTGAGGAGATGGCTCAGACATTCAGACAGAGAATTGCGCAAGCAGAAGCCATGGCTCGTGCAGACATGCTTCGCTTCCAACCTGAG

upper sequence: GRMZM5G862107_T01 (Zea mays), 3'ss of exon 5
lower sequence: Vv05s0077g00450.t01 (Vitis vinifera), 3'ss of exon 5

----------gtaagaataaacaaaagcaacatttt-ctcatgtgctgaatatgctttaggtaagcaactgacatatggtttct---tttgctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG
            |   |||||| | |      | || ||| | | |  | |     |   || |   |   | |  ||| |  |     || || ||  |||| || ||||||||||||| ||||||||||||||||||||| ||||| ||||||||||| || |||||| | |||||||| || |||
ttctgttacttcacctataaaccacacatgtgtgttgctcctctacctatttgaaatccagtgatttaacaatacttggctcttaaccctgttctaac-agGCGGAGGAGATGGCTCAGACATTCAGGCAGAGAATAGCTCAAGCAGAAGCTATGGCTCGGGCAGACATGCTAAGATTCCAACCTGAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|116813883|gb|EC856377.1|EC856377

EST:     AGCCAACACCTGGTGACATGATCCGCAATCCCAAGCTTGTGTTTGAGAAG                         GCTGATGAGATGGCTCAGATATTCAGGCAGCGAATAGCTCAGGCAGAGGCT
genomic: AGCCAACACCTGGTGACATGATCCGCAATCCCAAGCTTGTGTTTGAGAAGgtaagaataa ... ctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCT

EST: gi|4646619|gb|AI491439.2|AI491439

EST:     AGCCAACACCTGGTGACATGATCCGCAATCCCAAGCTTGTGTTTGAGAAG                         GCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCT
genomic: AGCCAACACCTGGTGACATGATCCGCAATCCCAAGCTTGTGTTTGAGAAGgtaagaataa ... ctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCT

EST: gi|32935407|gb|CF040219.1|CF040219

EST:     AGCCAACACCTGGTGACATGATCCGCAATCCCAAGCTTGTGTTTGAGAAG                         GCTGATGAGATGGCTCAGATATTCAGGCAGCGAATAGCTCAGGCAGAGGCT
genomic: AGCCAACACCTGGTGACATGATCCGCAATCCCAAGCTTGTGTTTGAGAAGgtaagaataa ... ctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 96

GAGCCATGACCGTGAAAGAGGCCGAGTCAGCCTTTCTACTAAGAAGCTTGAGCCAACACCTGGTGACATGATCCGCAATCCCAAGCTTGTGTTTGAGAAGgtaagaataa ... ctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 714

Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 38

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 257

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tgctgaatatgctttaggtaagcaactgacatatggtttcttttgctcaaattagGCTGATGAGATGGCTCAGATATTCAGGCAGAGAATAGCTCAGGCAGAGGCTATGGCTCGTGCTGACATGTTGAGATTCCAGCCAGAG