ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtatgttcttccattctgggttctctgattattgtctacactgctgaaacacttgtcatttgtctgtggtaaattttatatttcttcctgactgttggctaatgttttatgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTTCCGAGACAGAGACTACGATGTGGCAGCACTCACTAATAATATGAGCCAG

Basic information

species	Zea mays
transcript	GRMZM5G812498_T01
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM5G812498_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os05g51490.1 (Oryza sativa), 3'ss of exon 15

gtatgttcttccattctgggttctctgattattgtctacactgctgaaacactt-gtcatttgtctgtggtaaattttatatttcttc-ctgactgttggcta---atgttttatgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTTCCGAGACAGAGACTACGATGTGGCAGCACTCACTAATAATATGAGCCAG
                                    ||   |      |   || || ||   | |||| |    |  |  | ||||| |||||| |||||||   ||| ||| |||||||| ||||| || ||||||||||||||||| |||||||||||||| || |||||||||||||| ||||| |||||||| |||||  |||||||  ||||||||
-----------------------------------gtaggttatcaccatgttttgtgatgcttttgtgattgcgtacacttgtcttcgctgacttttggctatcaatgctttgtgctgcagTCGTCCGACTTCTCTACATGATCGTGGTAGGGATAGCGATGATGATGACTTCCGAGACAGGGACTATGATGTGGCGGCACTTGCTAATAACCTGAGCCAG

upper sequence: GRMZM5G812498_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os01g40370.1 (Oryza sativa), 3'ss of exon 2

gtatgttcttccattctgggttctctgattattgtctacactgctgaaacacttgtcatttgtctgtggtaaattttatatttcttcctgactgttgg-cta-atgtttta-tgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTTCCGAGACAGAGACTACGATGTGGCAGCACTCACTAATAATATGAGCCAG
                  ||        |  |||| |    || |   |  ||    | |||  |||  | | || | | |||   |   |  |||   ||| |||| ||  |||| ||| || |||||||| || |||||  |||| |||||||| ||||| ||||||||||||||||  || || ||||||| ||| || ||||||||  ||||||||
------------------ggcatgtgactctttgttttttttgattgtatgctaa--acttggatgtctttacttctgttttttcactgaatagtttctctatatgtcttgctgctacagGCGCCCAACATCACTTCATGACTGTGGTAGGGATAGTGATGATGACGACTTCCGAGACATGGATTATGATGTGGTAGCTCTTACTAATAACTTGAGCCAG

upper sequence: GRMZM5G812498_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os01g40390.1 (Oryza sativa), 3'ss of exon 4

gtatgttcttccattctgggttctctgattattgtctacactgctgaaacacttgtcatttgtctgtggtaaattttatatttcttcctgactgttgg-cta-atgttttat-gctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTTCCGAGACAGAGACTACGATGTGGCAGCACTCACTAATAATATGAGCCAG
                  |  ||| || || || | |    || ||  |  ||   |  |||  |||  |   || | | |||   |   |  |||   ||| |||| || | ||||||| |  |||||||| || ||||| | ||| |||||||| ||||| || || ||||||||||| || || ||||||||||| ||  |||||||  ||||||||
---------------tgtgactctttgtttttttttt---ttgatggcatgctaacc--ttggatgtctttgcttctgttttttcactgaatagtttctctatatgtcttgttgctgcagGCACCCAACATCACTTCATGACCATGGTAGGGATAGTGATGATGATGATTTCCGAGACAGGGATTATGATGTGGCAGCTCTTGCTAATAACTTGAGCCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|18659647|gb|BM500448.1|BM500448

EST:     GTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGG                         CCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTT
genomic: GTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGGgtatgttctt ... tatgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTT

EST: gi|13388459|gb|BG462175.1|BG462175

EST:     GTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGG                         CCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGAC
genomic: GTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGGgtatgttctt ... tatgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGAC

EST: gi|78110602|gb|DV529010.1|DV529010

EST:     GTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGG                         CCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTT
genomic: GTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGGgtatgttctt ... tatgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTT

EST: gi|60399315|gb|DN232129.1|DN232129

EST:     GTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGG                         CCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTT
genomic: GTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGGgtatgttctt ... tatgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 72

GAGAACAGTGAGTGGGCTGTATGGCAAATAGATGTCCTGATCAAACGCAATGAGGTGGAGAATGTTTACCATTGGGCATGTGGgtatgttctt ... tatgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTTCCGAGACAGAGACTACGATGTGGCAGCACTCACTAATAATATGAGCCAG

Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 56

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 67

Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 55

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tctgtggtaaattttatatttcttcctgactgttggctaatgttttatgctgcagCCGTCCAACATCTCTACATGATCGTGGGAGGGATAGCGATGACGACGACTTCCGAGACAGAGACTACGATGTGGCAGCACTCACTAATAATATGAGCCAG