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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tctaacagataatgataagtactgaagcaactggctatggatttcttatcttcattttcctgatatttttatactaagacttctcttgcttcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTCATGATACTGGGGCCACCAATCGTGGCTGCTATCATCTACATAGTACAG

Basic information

species Zea mays
transcript GRMZM2G702159_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G702159_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os02g45650.1 (Oryza sativa), 3'ss of exon 5
-----------------------------------------------------------------tctaacagataatgataagtactgaagcaact--ggctatggatttcttatcttcattttcctgatatttttatactaagacttc--tcttgcttcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTCATGATACTGGGGCCACCAATCGTGGCTGCTATCATCTACATAGTACAG
                                                                  ||   |||| | |  ||||||  ||| | ||  |  |||| ||||  ||| |||   | ||  | || ||||| || |  ||      |||      ||  |||||||||| ||||||||||| ||||| |||||||||||  |||| |||| |||  |||| || || || || ||||||||||| |||  ||||||||||
gtatgtttgtgttctcaattttagcacctcagttattatatgttttaacatttgaatggtgttcacctgcaagatta-gtgaagtacaaaagtatcttggcttatgaatttgctatgttctaatccccaacatatttattctgaacctctagctttggacttttttcagCAAACTATTTGGCTCTTCATTAGGGACATGATCAAAGGGATTTTGCTATCCATCTTACTTGGACCTCCTATTGTGGCTGCTATTATCATCATAGTACAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|40286942|gb|CK327334.1|CK327334
EST:     CTCTATTTATTCAACTTTTGGTTATAGAGGCTCGACATGGTTTTAACAAG                         CAAACTATATGGCTCTTCATCAGGGATATGATCAANGGAGTTTTACTATTC
genomic: CTCTATTTATTCAACTTTTG-TTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTC
EST: gi|40286924|gb|CK327316.1|CK327316
EST:     TCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAG                         CAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAATTTTACTATCC
genomic: TCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTC
EST: gi|91875692|gb|EB405649.1|EB405649
EST:     TCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAG                         CAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTC
genomic: TCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTC
EST: gi|34391316|gb|CF384544.1|CF384544
EST:     TCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAG                         CAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTC
genomic: TCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTC
EST: gi|101394395|gb|EB819132.1|EB819132
EST:     TCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAG                         CAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTC
genomic: TCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 45 (upstream exon), 43 (downstream exon)
Score: 28

ATTACAGACTTGTCGTTCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTCATGATACTGGGGCCACCAATCGTGGCTGCTATCATCTACATAGTACAG


Block sizes: 31 (upstream exon), 40 (downstream exon)
Score: 3

ATTACAGACTTGTCGTTCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTCATGATACTGGGGCCACCAATCGTGGCTGCTATCATCTACATAGTACAG


Block sizes: 40 (upstream exon), 43 (downstream exon)
Score: 12

ATTACAGACTTGTCGTTCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTCATGATACTGGGGCCACCAATCGTGGCTGCTATCATCTACATAGTACAG


Block sizes: 41 (upstream exon), 41 (downstream exon)
Score: 16

ATTACAGACTTGTCGTTCTCTATTTATTCAACTTTTGTTATAGAGGCTCGACATGGTTTTAACAAGgtgcgttcac ... tcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTCATGATACTGGGGCCACCAATCGTGGCTGCTATCATCTACATAGTACAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttatcttcattttcctgatatttttatactaagacttctcttgcttcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTCATGATACTGGGGCCACCAATCGTGGCTGCTATCATCTACATAGTACAG
            tcctgat  putative branch site (score: 16)
                                  cttctcttgcttccct  putative PPT
               tgatatttttatacta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tctaacagataatgataagtactgaagcaactggctatggatttcttatcttcattttcctgatatttttatactaagacttctcttgcttcccttgtagCAAACTATATGGCTCTTCATCAGGGATATGATCAAAGGAGTTTTACTATTCATGATACTGGGGCCACCAATCGTGGCTGCTATCATCTACATAGTACAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGCT
- - - - - - - - - - -ctgaagc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ctcttgc