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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagctaaaattagctatcttgaacctaacgttcttagatgtatgtattactattgatttacactatgatcatctgtttgccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG

Basic information

species Zea mays
transcript GRMZM2G458035_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G458035_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os07g07560.1 (Oryza sativa), 3'ss of exon 7
gtgagctaaaattagctatcttgaacctaacgttcttagatgtatgtattactattgatttacactatgatcatctgtttgccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG
|| |  ||  ||      |||| | |      | |||||||| |      | | || |||||             ||||||  |||||||||||||||||||||||||||||||| ||||||||||| ||||||||||| |||||  | ||||||||||| ||||  |||||||||||||
gtaacttattatctttgttctttatcaggttatccttagatgcac----cattcttaattta-------------tgtttgt-agGTTTGTGCATGGTGATGTGAAACCAGAGAACTTTCTGCTTGGCCAGCCTGGATCACCTGACGAGAAGAAGCTTTATCTTATTGATCTTGGTTTAG

upper sequence: GRMZM2G458035_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA16G04580.1 (Glycine max), 3'ss of exon 7
-----------------gtgagctaaaattagctatcttgaac-ctaacgttc--ttagatgtatgtattact-attgatttacacta-tga---tcatctgtttg--ccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG
                  |     ||   |  | | ||| |||  || | ||   || |   |   | | ||| | | ||||  | || |||   || | |  |||    |||||||||||||| ||||||||||| || || ||| |||||||||| ||||||||  ||||| |||||||| | || ||||  ||||||||| | |
gtaaacccttgtgatagttttttcaatcctgtcaacctttaacattaccatttattttggcatggttgtgactgactaatttttattaatgaaactcgtatcattgaattagGTTTGTGCATGGAGATGTGAAACCTGAAAACTTTTTGCTTGGTCAACCTGGATCAGCTGAAGATAAGAAGTTATATCTTATTGATCTTGGTCTGG

upper sequence: GRMZM2G458035_T01 (Zea mays), 3'ss of exon 5
lower sequence: AT3G03940.1 (Arabidopsis thaliana), 3'ss of exon 7
----------------------------------------gtgagctaaaattagctatcttgaacctaacgttcttagatgtatgtattactattgatttacactatg-atcatctgttt---gccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG
                                         |     |||| ||  ||  ||    | ||     |  |   |   |      | ||   | || | |  ||   ||   |    | |||||||||||||| |||||||||||||| || ||| ||||||||||||||||| |  | ||  | ||||| || |||||||  ||||||||| |||
gttagtgcgaatttttgttgtcgccaataaatccatctatttcgaacaaaaatatttacatttgcaccaaaaaattacgccattgctgagctgactgtcatgcattgttcattccctacatcaaacaagGTTTGTGCATGGAGATGTGAAACCAGAAAACTTTTTGCTTGGTCAGCCTGGAACAGCAGACGAGAAGAAACTATACCTTATCGATCTTGGTCTAG

upper sequence: GRMZM2G458035_T01 (Zea mays), 3'ss of exon 5
lower sequence: Vv08s0007g07420.t01 (Vitis vinifera), 3'ss of exon 7
----------------------------------gtgagctaaaattagctatcttgaacctaacgttcttagatgtatgtattactattgatttac-actatgatcatctgtttgcc-------agGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG
                                        | |   |  || |||||| |   || ||| |  | ||   |  | |  |   || |  | | | ||| | | | | ||       |||||||||||||| |||||||| |||||||| ||| | |||||||| |||||| |  ||||  | |||||||| |  ||||  ||||||||||| |
gtgagtactattaataattccatgtttcactttctcagaatcatcctgtctctcttgagc---actttcctcaaagtggcttcttcattctgttaattattgtcatcttttttcttcctcaaccaagGTTTGTGCATGGAGATGTGAAGCCAGAGAACTTTTTACTTGGTCAACCTGGAACTGCTGATGAGAAGAAGCTATTTCTTATTGATCTTGGTTTGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 278

GACTAAACGAGTCAACAATCTCAAACAGGATGACTCCAAATATGGCTGCTTGCATTGCTGTAGAGAGCATATCAATTCTTGAGAAGCTCCATTCTAAAGGgtgagctaaa ... tgtttgccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 162

GACTAAACGAGTCAACAATCTCAAACAGGATGACTCCAAATATGGCTGCTTGCATTGCTGTAGAGAGCATATCAATTCTTGAGAAGCTCCATTCTAAAGGgtgagctaaa ... tgtttgccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 155

GACTAAACGAGTCAACAATCTCAAACAGGATGACTCCAAATATGGCTGCTTGCATTGCTGTAGAGAGCATATCAATTCTTGAGAAGCTCCATTCTAAAGGgtgagctaaa ... tgtttgccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 172

GACTAAACGAGTCAACAATCTCAAACAGGATGACTCCAAATATGGCTGCTTGCATTGCTGTAGAGAGCATATCAATTCTTGAGAAGCTCCATTCTAAAGGgtgagctaaa ... tgtttgccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cgttcttagatgtatgtattactattgatttacactatgatcatctgtttgccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG
                      tattgat  putative branch site (score: 172)
                                           tctgttt  putative PPT
                tattactattgattta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagctaaaattagctatcttgaacctaacgttcttagatgtatgtattactattgatttacactatgatcatctgtttgccagGTTTGTGCATGGTGATGTGAAACCAGAGAATTTTCTGCTTGGTCAGCCTGGATCGCCTGAATATAAGAAGCTTTACCTTACAGATCTTGGTTTAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGC