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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaatttctaatgttctttccttgcatggacacatatttttttccgtagtgctaatttaatgccctatcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTTTCTGCTGCTTCTTACCCAGACCTGATAGATGCGTTGCCGAAAAATCCAC

Basic information

species Zea mays
transcript GRMZM2G407044_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G407044_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os02g39570.5 (Oryza sativa), 3'ss of exon 5
------------gtaatttctaat-gttctttccttgcatggacacatatttttttccgtagtgctaatttaatgccctatcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTTTCTGCTGCTTCTTACCCAGACCTGATAGATGCGTTGCCGAAAAATCCAC
              | |||||| | |   ||  | |||||| | |||| | || |||   |||||| | |   |   |   |  || |||||||||||||||||||||  ||||| ||||| ||||| |||||| ||||||||||||||||||||||||| || ||||| || ||||| |||||| | |
gtattttcttgaccattttctatttgcatttatcatgcatgcatacatgtgttattc-acagtgctgactcttttttc---cccctcagATTGCTTTGAGACGTGAAAAAATTGGAGCAACTGCCCGCTTCTGGGGATTTTCTGCTGCTTCTTACCCAGATCTCATAGAGGCATTGCCCAAAAATTCTC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|33098451|gb|CF058411.1|CF058411
EST:     AGAGGAGTCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAA                         ATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
genomic: AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
EST: gi|149075260|gb|EE163764.2|EE163764
EST:     AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAA                         ATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
genomic: AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
EST: gi|78122764|gb|DV541148.1|DV541148
EST:     AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCGGGACAGGAAAA                         ATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
genomic: AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
EST: gi|148949305|gb|EC894756.2|EC894756
EST:     AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAA                         ATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
genomic: AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
EST: gi|71435773|gb|DR816823.1|DR816823
EST:     AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAA                         ATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
genomic: AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT
EST: gi|149087824|gb|EE177122.2|EE177122
EST:     AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAA                         ATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCC-GTTTCTGGCGATTT
genomic: AGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 226

GTAGCTGGAGATCCTGGCAAAATTGCTGCAGTGCAGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTTTCTGCTGCTTCTTACCCAGACCTGATAGATGCGTTGCCGAAAAATCCAC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 122

GTAGCTGGAGATCCTGGCAAAATTGCTGCAGTGCAGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTTTCTGCTGCTTCTTACCCAGACCTGATAGATGCGTTGCCGAAAAATCCAC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 43

GTAGCTGGAGATCCTGGCAAAATTGCTGCAGTGCAGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTTTCTGCTGCTTCTTACCCAGACCTGATAGATGCGTTGCCGAAAAATCCAC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 123

GTAGCTGGAGATCCTGGCAAAATTGCTGCAGTGCAGAGGAATCTATGGAAATTTGGAATCAAAGAAATTTGCAGGACAGGAAAAgtaatttcta ... atcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTTTCTGCTGCTTCTTACCCAGACCTGATAGATGCGTTGCCGAAAAATCCAC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttgcatggacacatatttttttccgtagtgctaatttaatgccctatcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTTTCTGCTGCTTCTTACCCAGACCTGATAGATGCGTTGCCGAAAAATCCAC
                                       tgccctatcttctt  CT-rich tract
        acacatattttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaatttctaatgttctttccttgcatggacacatatttttttccgtagtgctaatttaatgccctatcttcttagATTGCTTTGAGACGTGAAAGGATTGGTGCAACAGCCCGTTTCTGGCGATTTTCTGCTGCTTCTTACCCAGACCTGATAGATGCGTTGCCGAAAAATCCAC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGCT