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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttgacagtaaaatcattttgttcttcactcatgaaggttccctcatatggtcccaaatttaaaatatactttattcaatggatatcgatgattttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT

Basic information

species Zea mays
transcript GRMZM2G366935_T05
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G366935_T07 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os01g40840.2 (Oryza sativa), 3'ss of exon 4
ttgacagtaaaatcatt-ttgttcttcactcatgaaggttccctcatatggtcccaaatttaaaatatactttattcaatggatatcgatgattttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT
 ||    |||||   || | ||||||  ||||| ||||||  |  ||||||   |||||  || |||   ||| ||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
-tgcttataaaacagttgtcgttctttgctcataaaggtttgccgatatggaaacaaatcaaagataatttttcttcaatggatatcgatggttttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCAACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT

upper sequence: GRMZM2G366935_T07 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA06G08480.2 (Glycine max), 3'ss of exon 4
ttgacagtaaaatcattttgttcttcactcatgaaggttcc-ctcatatggtcccaaattta--aaatatactttattcaatggatatcgatgattttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT
   | |||  | |  ||||  | |     ||||  ||||    ||||    |    || | |  | | | | |   ||||||| || |  ||| | || |||||| |||||||||||||| |||||||| | |||||||||||||||||||| |||| | || ||||||||||| ||||| ||||| |||||||| || || |
--tatagtgga-ttgtttttatatatgtccatgtgggttttattcatggatttgataactaagcagaaacattcctttcaatgcattttaatggtgttatcagGTCTTTGAGAAGCTTGGACCAAGCTTATTTGATTTTCTAAAGAGAAATAAATACTGCCCATTCCCTGTGGATCTTGTTCGGGAATTTGGACGTCAGCTTT

upper sequence: GRMZM2G366935_T07 (Zea mays), 3'ss of exon 4
lower sequence: AT4G32660.1 (Arabidopsis thaliana), 3'ss of exon 5
--ttgacagtaaaatcattttgttc-ttcactcatgaaggttccctcatatggtcccaaatttaaaatatactttat-tcaatggatatcgatgattttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT
  |  | |||    |  | | |||  || |||||||  | ||   ||  |  ||    || | ||   |  |||  | | |||| |     ||| |  |  ||||| || ||||||||||||||||| |||| ||| |||||||||||||||| ||||    | || ||| |||  ||||| ||||| |||||| | || || |
cgtgtatagtgt--ttgtgtagtttgtttactcatgtggattttttcgaa--gtttggaactaaacggaaccttcctgtgaatgaactctaatggtgataccagGTGTTCGAGAAGCTTGGGCCAAGTTTGTTTGACTTTCTAAAGAGAAATAAATACTCCGCATTTCCTCTGGCCCTTGTTCGGGATTTTGGATGCCAGCTTT

upper sequence: GRMZM2G366935_T07 (Zea mays), 3'ss of exon 4
lower sequence: Vv04s0008g04220.t01 (Vitis vinifera), 3'ss of exon 5
ttgacagtaaaatcattttgttcttcactcatgaaggttccctcatatggtcccaaatttaaaat----atactttattcaatggatatcgatgattttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT
       ||   | | ||  ||  || |   || |  |||  ||||| |     ||| |||| ||    ||  |   || |||| |  |  ||| | ||  ||||| |||||||||||||| ||||| || | |||||||||||||||||||| |||| | || || |||||||| ||||| ||||| |||||||| || || |
--tgtgatagtttgaattaatttctc-cctgtggac-ttcattcataagactggaaacttaacattgaaatcttcctttgaatgcactttaatggtgttaccagGTGTTTGAGAAGCTTGGACCAAGTTTATTTGATTTTCTAAAGAGAAATAAATACTGCCCATTTCCTGTGGATCTTGTTCGGGAATTTGGACGTCAGCTTT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|89248362|gb|DY620148.1|DY620148
EST:     CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATT                         GTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTC
genomic: CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATTgtaagtacta ... attttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTC
EST: gi|21331443|gb|BQ486824.1|BQ486824
EST:     CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATT                         GTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGA
genomic: CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATTgtaagtacta ... attttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGA
EST: gi|148930529|gb|EE285130.2|EE285130
EST:     CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATT                         GTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGA
genomic: CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATTgtaagtacta ... attttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 284

CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATTgtaagtacta ... attttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT


Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 188

CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATTgtaagtacta ... attttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 178

CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATTgtaagtacta ... attttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 199

CTGTGTTCAAATACAGAGATGGTTTGACTATCGTAATCATATATGCATTgtaagtacta ... attttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tatggtcccaaatttaaaatatactttattcaatggatatcgatgattttgtcagGTTTTTGAGAAGCTTGGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGT
                                              ttttgtc  CT-rich tract
         aaatttaaaatatact  TA-rich tract