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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagacggcagagctcaaaatagaaagttgaattgataatagtcgtggcgatattagtaattggtgcctgacattgttttttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG

Basic information

species Zea mays
transcript GRMZM2G323622_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G323622_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os01g54080.1 (Oryza sativa), 3'ss of exon 14
----gtaagacggcagagct----caaaatag-aaagttgaattgataatagtcgt-ggcgatattagtaattggtgcctgacattgttttttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG
     ||  || |   | ||    ||| | || |||  ||   || |||   || | || ||||||   |||||    ||   ||  ||||      |||||||| ||||| ||||||| |||||| ||||||||||||||||| |||||||||||  ||||| |||| ||||||||||| |  ||||| ||||||||||
gtatttactactgtttaactaattcaagacagcaaacctgctctgctaactatcattggggatattgtaaattgac-tctcttatc-ttttaccctttagAGTGGACCAGGGACATCAAGAGAAGACTGGGGTGTTAACTATCGTGCTTTAAATGATCTGTTTGATATTTCTCTAAGTAGGAAAAATGCATTCTCATATG

upper sequence: GRMZM2G323622_T01 (Zea mays), 3'ss of exon 5
lower sequence: AT5G41310.1 (Arabidopsis thaliana), 3'ss of exon 12
----------gtaagacggcagagctcaaaatagaaagttgaattgataatagtcgtggcgatat-tagtaattggtgcctgacattgttttttttcctagAGTGGGCCAGGCACA---TCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG
          ||       ||   |      |||||| |||  | || | |   | ||      | ||||   |        |  | ||   ||  |  ||||||| ||| ||| |   ||| |||||||| |||||||||||||||||||||| |||||||||||  |  |  ||| |||||||   ||  ||   | || | 
tttcgtaaatgtttttgaccatttcattggttagaaacttgg-tcgaaattccacatgctatcttgtagtggataagttaaaaattagtgaattaacacagAGTGGACCAAGCATAACTTCAGAAGAAGATCGGGGTGTTAACTATCGAGCTTTGAATGACTTGTTCCACTTAACTCAAAGTAGACAAAA--CTCAGTGATGT-

upper sequence: GRMZM2G323622_T01 (Zea mays), 3'ss of exon 5
lower sequence: AT1G63640.1 (Arabidopsis thaliana), 3'ss of exon 14
gtaagacggcagagctcaaaatagaaagttgaattgataatagtcgtggcgatattagtaattggtgcctgacattgttttttttcc-----tagAGTGGGCCAGGCA---CATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG
        | | |   |||  | ||   |   |  |||| |  |     | ||  |  ||| |  |  || ||| | | | |||| |      ||||||||||| |||   | ||||||||||| |||||||| || ||| ||||||| ||||||||||||    |  |||  ||||||   ||  || ||   |   
--------gtaaattacaagcttgattttgttaacgata-tgctttaaccaatgattataagtt-tttctaacagtttctattttgcaaacacagAGTGGGCCAAGCATCACTTCAAAAGAAGACTGGGGTGTCAATTATAGAGCTTTGAATGACTTGTTTCTCTTAACTCAGAGTAGACAAAACACTGTCATGT---

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211110697|gb|FL352656.1|FL352656
EST:     TGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATG                         AGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTA
genomic: TGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATGgtaagacggc ... tttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTA
EST: gi|211353939|gb|FL310943.1|FL310943
EST:     TGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATG                         AGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTA
genomic: TGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATGgtaagacggc ... tttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTA
EST: gi|38687291|gb|CK144322.1|CK144322
EST:     TGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATG                         AGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTA
genomic: TGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATGgtaagacggc ... tttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTA
EST: gi|211021362|gb|FL343609.1|FL343609
EST:     TGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATG                         AGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTA
genomic: TGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATGgtaagacggc ... tttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 466

ATTCTCTGATATCCAGCCTCTGATCAGATCAGTTCTTGATGGGTTTAATGTGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATGgtaagacggc ... tttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG


Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 80

ATTCTCTGATATCCAGCCTCTGATCAGATCAGTTCTTGATGGGTTTAATGTGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATGgtaagacggc ... tttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 63

ATTCTCTGATATCCAGCCTCTGATCAGATCAGTTCTTGATGGGTTTAATGTGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATGgtaagacggc ... tttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 321

ATTCTCTGATATCCAGCCTCTGATCAGATCAGTTCTTGATGGGTTTAATGTGTGCATTTTTGCCTATGGTCAAACTGGTTCAGGAAAGACTTACACAATGgtaagacggc ... tttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gataatagtcgtggcgatattagtaattggtgcctgacattgttttttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG
                               gcctgac  putative branch site (score: 321)
                                     cattgttttttttcct  CT-rich tract
                                    acattgtttttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagacggcagagctcaaaatagaaagttgaattgataatagtcgtggcgatattagtaattggtgcctgacattgttttttttcctagAGTGGGCCAGGCACATCAAAAGAAGATTGGGGTGTTAACTATCGAGCTTTAAATGACTTGTTTTATATCTCTCTAAGTAGAAGGAATGCTTTCTCATATG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAA