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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagcggcggtactgcgttttcgctgttccgactgtgtcttgttccggaaattttgaaagatttcttagtaactaatgtttttgtcgtccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCCGATGAAAAAGTTTCCGAGTGCGTGAAAGAAGTGGTCTGCTGCAGTTCTG

Basic information

species Zea mays
transcript GRMZM2G165044_T01
intron # 5
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149082639|gb|EE171841.2|EE171841
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|74234292|gb|DT642206.1|DT642206
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|148928371|gb|EE285057.2|EE285057
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|149037193|gb|EE155279.2|EE155279
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|148963606|gb|EE014646.2|EE014646
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|148938218|gb|EC882505.2|EC882505
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|148943118|gb|EC888176.2|EC888176
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|71766106|gb|DR964043.1|DR964043
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|148999876|gb|EE036910.2|EE036910
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|71420925|gb|DR805237.1|DR805237
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|94477093|gb|EB706051.1|EB706051
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|71760265|gb|DR958202.1|DR958202
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|86466629|gb|DY232999.1|DY232999
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|149042041|gb|EE047016.2|EE047016
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|148949435|gb|EC894894.2|EC894894
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|71774351|gb|DR972229.1|DR972229
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|78110998|gb|DV529395.1|DV529395
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|94477752|gb|EB706710.1|EB706710
EST:     GGACATGAGACTGGACATGGACAACATGAGCTGCGAG                         GACCTGTGGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: GGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|74232028|gb|DT639942.1|DT639942
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|76921859|gb|DV168737.1|DV168737
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|148961832|gb|EE012614.2|EE012614
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|71436822|gb|DR817872.1|DR817872
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|88755341|gb|DY539482.1|DY539482
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|76925038|gb|DV170262.1|DV170262
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|94476101|gb|EB705059.1|EB705059
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|149014262|gb|EE043830.2|EE043830
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|148934246|gb|EC878267.2|EC878267
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|71758169|gb|DR956106.1|DR956106
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|149082698|gb|EE171913.2|EE171913
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|76921857|gb|DV168736.1|DV168736
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|89248748|gb|DY620534.1|DY620534
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|50323081|gb|CO518207.1|CO518207
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|88755287|gb|DY539428.1|DY539428
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|110533295|gb|EE037475.1|EE037475
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|33467803|gb|CF244852.1|CF244852
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|50330081|gb|CO525207.1|CO525207
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|149102323|gb|EE287689.2|EE287689
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|71434518|gb|DR815568.1|DR815568
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|86472396|gb|DY238766.1|DY238766
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
EST: gi|71766107|gb|DR964044.1|DR964044
EST:     CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAG                         GACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC
genomic: CTGACCCCCACAGGGACATGAGACTGGACATTGACAACATGAGCTACGAGgtaagcggcg ... ccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCC


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tccggaaattttgaaagatttcttagtaactaatgtttttgtcgtccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCCGATGAAAAAGTTTCCGAGTGCGTGAAAGAAGTGGTCTGCTGCAGTTCTG
                                            tccgtcc  CT-rich tract
     aaattttgaaagattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagcggcggtactgcgttttcgctgttccgactgtgtcttgttccggaaattttgaaagatttcttagtaactaatgtttttgtcgtccgtccatagGACCTGTTGGCTCTGGGAGAAGCTATGGGCAATGTCAACACAGGCCTGGCCGATGAAAAAGTTTCCGAGTGCGTGAAAGAAGTGGTCTGCTGCAGTTCTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGCAG