ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

gtttgtgctatgtatcatcactactctccatatttggagagtttacagtgtgcttgaggagtgagtgccgcagtgcagacgaagtgagaagtgaccacttctcactcattcattttattggtttcattttactctcctgattagatatagacctgacttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC

Basic information

species	Zea mays
transcript	GRMZM2G155348_T01
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G155348_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os01g71280.1 (Oryza sativa), 3'ss of exon 5

gtttgtgctatgtatcatcactactctccatatttggagagtttacagtgtgcttgaggagtgagtgccgcagtgca-gacgaagtgagaagtgaccacttctcactcattcattttattggtttcattttactctcctgattagatatagac--ctgactt-atgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC
                                                                         | || | | | || |    | | |  ||   | | | | | | ||     |   ||| |  |  |    ||  | ||     |||  || || | |||||||||| |  |||| || ||||| ||||||||||| || ||||| |||||||||||||| ||||||||||||||||| |||||||||||||||||||||
----------------------------------------------------------------------tcttacatgtcaaggtcaatcatcaactttttcta-taacttagtataacacatctgtttcaaccagcaatgtatctttaactcactgttttgatctacagGGAGCTCTCGACAAGAGATATTCTGCAAGCCCTGAGAGATGAAACCATGAATGATCCTCGTGAAAGGATTGAGATGGCGCAAAGCCATGCATTCTACCGC

upper sequence: GRMZM2G155348_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA10G15900.1 (Glycine max), 3'ss of exon 4

gtttgtgctatgtatcatcactactctccatatttggagagtttacagtgtgcttgaggagtgagtgccgcagtgcagacgaagtgagaagtgaccacttctcactcattcattttattggtttcattttactctcctgattagatatagacctgacttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC
                                                             |  |||    | |  | |  ||   |  | | |  | |   ||   | | |  |    ||  | | |||||  | ||  |  |||  |      | || |   |||||| |   || ||   |  ||||| ||||| || ||||| ||||   |||||||||| || ||| | |||||||| |||||||||||||| ||||||   
-----------------------------------------------------------tatatgtgaataattaaaaataaa---aagactaaaaattcgacataaactaaaatataaggactgaatttaccttgctact--gatgcattttgcatttct---cagGGAACAGTCATCAGAAGCTATTCTCCAAGCTCTTAGGGATGAAAATCTGAATGATCCCCGTGAACGCATTGAGATTGCACAAAGCCATGCTTTCTACATG

upper sequence: GRMZM2G155348_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA02G33340.1 (Glycine max), 3'ss of exon 5

gtttgtgctatgtatcatcactactctccatatttggagagtttacagtgtgcttgaggagtgagtgccgcagtgcagacgaagtgagaagtgaccactt--ctcactcattcattttattggtttcattttactctcctgattagatatagacctgacttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC
                                                                       | || | |  || | |    | |  |||     | | |    |   |||   |   | |||  |  |||   ||   ||  |  |  | | |   |||||| |    | |    |  ||||| ||||| || ||||| ||||   ||||||||||||||||| | |||||||| |||||||||||||| ||||||   
-------------------------------------------------------------------taaaattgtacataaattaaagtataaggactggatccgcgcgcctagcctat-actacaagtttctttaccttgctactgatgcattttgcatttcc-cagGGAACAGTTATCGGAAGCTATTCTCCAAGCTCTTAGGGATGAAAATCTGAATGATCCTCGGGAACGCATTGAGATTGCACAAAGCCATGCTTTCTACATG

upper sequence: GRMZM2G155348_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA03G28970.1 (Glycine max), 3'ss of exon 4

gtttgtgctatgtatcatcactactctccatatttggagagtttacagtgtgcttgaggagtgagtgccgcagtgcagacgaagtgagaa-gtgaccacttctcactcattcattttattggtttcattttactctcctgattagatatagacctgacttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC
           |||   || || |      |   || |   ||   ||| || ||      |   | ||    |||     |  |  |||     |||  ||  | |  | ||||| |     | | ||  |   | | |||  |   || | ||          |||||| |   || ||   |  |||||  ||||| ||||||| ||||   ||||||||||||| ||| | |||||||| |||||||||||||  ||||||   
-----------gtaatctc-ctgcaacaattgactgaaaccttggtagtctgttt------tctttacccttatgcttgttatctttgaacacttccaactccaagtactacatttgaaaatctcctttacatcttgccgat--gcactaaatct----------cagGGAACAGTCATCACAAGCTATTCTGAAAGCCTTGAGGGATGAAAATCTGAATGATCCTCGTGAACGAATTGAGATTGCACAAAGCCATGTTTTCTACAAG

upper sequence: GRMZM2G155348_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA19G31730.1 (Glycine max), 3'ss of exon 4

----gtttgtgctatgtatcatcactactctccatatttggagagtttacagt-gtgcttg--aggagtgagtgccgcagtgcagacgaagtg-----agaagtgaccacttctcactcattcattttattggtttcattttactctcctgatta-gatatagacctgacttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC
      |  ||| ||   | |     ||| | |   | |      |||||  |  ||||||  |    |||   |  |       | | | |      | |  ||   |  |   |  |||   |   ||    |  ||     || | || ||  | |  |  |  || || |   |||||| |   || ||   |  |||||  ||||| ||||||| ||||   ||||||||||||| ||| | |||||||| |||||||||||||  ||||||   
gtaatctcctgcaataattgactgaaactatgctagtcttctttctttacccttatgcttgttatctttgaacacttccaactccaagtactactactacatttgcaaatcttctatacatctttccaatgcactaaatgggtttcacatgtttctggtgcatgctggatttct---cagGGAACAGTCATCACAAGCTATTCTGAAAGCCTTGAGGGATGAAAATCTGAATGATCCTCGTGAACGAATTGAGATTGCACAAAGCCATGTTTTCTACAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|149110284|gb|EE294060.2|EE294060

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGAGGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|32928869|gb|CF033681.1|CF033681

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCCGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|149112969|gb|EE295754.2|EE295754

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTTTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|50325670|gb|CO520796.1|CO520796

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTTTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|148948103|gb|EC893612.2|EC893612

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|78089703|gb|DV518077.1|DV518077

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|149085936|gb|EE175464.2|EE175464

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|211249164|gb|FL479061.1|FL479061

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTYTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|78107372|gb|DV525790.1|DV525790

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGAGGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|113702398|gb|EE679902.1|EE679902

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGATGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|74237292|gb|DT645206.1|DT645206

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|148991616|gb|EE033150.2|EE033150

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTTTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|110501808|gb|EE016567.1|EE016567

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGAGGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|74245777|gb|DT653691.1|DT653691

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|149109827|gb|EE293621.2|EE293621

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|37375883|gb|CF624421.1|CF624421

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|60359720|gb|DN226693.1|DN226693

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|148957043|gb|EC903003.2|EC903003

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|78111933|gb|DV530329.1|DV530329

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|76014157|gb|DT941327.1|DT941327

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|44900398|gb|CK826943.1|CK826943

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|37390129|gb|CF632291.1|CF632291

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|110221136|gb|EC903034.1|EC903034

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|211437569|gb|FL434163.1|FL434163

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|74237291|gb|DT645205.1|DT645205

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

EST: gi|76908350|gb|DV162896.1|DV162896

EST:     GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAG                         GGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA
genomic: GCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 73

AACCCAGCTTAAGTGTGCAGCACCCAGAAGAAAACAAGCAGGTTGCTCCAGCTGAGCTATGCCCAATCCTGAAGAGGCTTTACAGAATACTGATAAAAAGgtttgtgcta ... ttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC

Block sizes: 49 (upstream exon), 39 (downstream exon)
Score: 22

Block sizes: 49 (upstream exon), 16 (downstream exon)
Score: 3

Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 184

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttttattggtttcattttactctcctgattagatatagacctgacttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC

                                      acctgac  putative branch site (score: 184)
         tttcatttta  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtttgtgctatgtatcatcactactctccatatttggagagtttacagtgtgcttgaggagtgagtgccgcagtgcagacgaagtgagaagtgaccacttctcactcattcattttattggtttcattttactctcctgattagatatagacctgacttatgtgcagGGAGCTCCCAGCAAGGGACATTCTTCAAGCCCTGAGGGACGAAACGATGAATGATCCTCGGGAAAGGATTGAGATGGCACAAAGCCATGCATTCTACCGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATG