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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgcgtggtttggtgtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttctgttccaaggcttattatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT

Basic information

species Zea mays
transcript GRMZM2G155242_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G155242_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os03g09250.2 (Oryza sativa), 3'ss of exon 5
gtgcgtggtttggtgtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttct--gttccaaggcttattatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
                                  || |   ||||    |  | |||   |||  |||   |||||  | | || ||||||||     ||||   |||    ||||||||| ||||||||||| || ||||||||| ||||||||||||| || ||||||||||||||||| |||||||| ||||||||||| |||||||| ||
----------------------------------gtatgtaattttctgtgtagagtagtatgcggtttattttctcaggttcatggcttatt-----tgtttactgtgtacagGGCTGATCGATCTTGCTATTAAGAACAACTGCCTGATTGGTGGTGATGATTTCAAGAGTGGACAGACAAAGATGAAGTCTGTCTTGGTTGATTTCCTAGT

upper sequence: GRMZM2G155242_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA18G02210.4 (Glycine max), 3'ss of exon 5
gtgcgtggtttggtgtgtttgcaaaagcttcatggtg--ttgcatttctgttccaaagtttcatggtgttgtatttctgtt---ccaaggcttattatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
                          | |  ||| |    |  || ||    ||| |  || |     |||||||| || |       |   | |  |   |||||  |||    |||||||||||||||||| ||    |  |||   ||||||||||| || |||||||||||||| |||||||| ||||||||||| ||||| ||||| |||||
--------------------------gttggatgcttgcctttatgtcccccccacaacttaaattgtttgtatttatgctaatttgtgaactttggtttgtgttgt-tgtcatcagGGCTGATTGATCTTGCCATCGCGAGGAACACTTTGATTGGTGGAGATGACTTCAAGAGTGGTCAGACCAAAATGAAATCTGTGTTGGTTGATTTTCTTGT

upper sequence: GRMZM2G155242_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA05G31450.2 (Glycine max), 3'ss of exon 5
gtgcgtggtttggtgtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttctgttccaaggcttattatacctgttgcatgttcgt-agGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
                |||    |  | ||  |  | ||  || |  |||   || ||| ||  |  || |       |  |  | | | | |  || |||  | ||||| ||||||||||||||| || || || |  ||| | |||||||| || || ||||| |||||||| |||||||| ||||||||||| ||||| |||||||||||
----------------gttcattattgattattattatt--atgtgggttattaaattttat--ttatgcaaaataaataaattggtgaattttgct-ttgtgttttcgtcagGGCTGATTGATCTGGCCATCAAGAGGAACAGTTTGATTGGAGGAGATGACTTTAAGAGTGGTCAGACCAAAATGAAATCTGTGTTGGTGGATTTCCTTGT

upper sequence: GRMZM2G155242_T01 (Zea mays), 3'ss of exon 5
lower sequence: AT4G39800.1 (Arabidopsis thaliana), 3'ss of exon 5
gtgcgtggtttggtgtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttctgttccaaggcttattatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
                          | ||||||  ||||  |||   |  |  |  || ||       ||||     || | | || ||| |    |  |  |||| ||||| || |||||| | || || |  |||||    ||||| ||||| || || ||||||||||| || ||||||||||||||||||||||| |||||||| ||
--------------------------gtttcatgtagttgagttttaatcactca--ttgatcaatcaatattggaaatc-atgactgattttcttgggggtttgtt-gtagGTCTAATTGATATGGCGATCAGGAACAATGTTTTGATCGGTGGAGATGATTTCAAGAGTGGTCAAACCAAGATGAAATCTGTCTTGGTTGATTTCCTAGT

upper sequence: GRMZM2G155242_T01 (Zea mays), 3'ss of exon 5
lower sequence: AT5G10170.1 (Arabidopsis thaliana), 3'ss of exon 5
gtgcgtggtttggtgtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttctgttccaaggcttattatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
                         | ||  ||     |  || |   |   |    || |||   ||  ||||    |   |  |  ||| | | | |   ||      |||||| |||||  | || ||||| |  ||||||||||| |||||||| |||||||||||||| |||||||||||||| |||||| | ||||||||||||||
-----------------------gtaactaaattaaactatatctagatagta----ttaatgaccttcaattttctatagtaatcacattttgcttatattatac----agGGCTTATTGAATTAGCCATAAAGAGAAACTGCTTGATCGGTGGTGATGACTTCAAGAGTGGTCAGACCAAGATGAAGTCTGTCCTCGTCGATTTCCTTGT

upper sequence: GRMZM2G155242_T01 (Zea mays), 3'ss of exon 5
lower sequence: Vv07s0031g00920.t01 (Vitis vinifera), 3'ss of exon 5
gtgcgtggtttggtgtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttctgttccaaggcttattat-acctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
|| | |  | ||   | ||| |       |      |||     |   |   | ||      || ||  || |     ||      || || | |    ||| ||||    ||||||||||||| | ||||| || |  ||  | ||||||||||| || || || |||||||| || ||||||||||||||||| ||||| |||||||||||
gttcttaatctgaaat-tttccttttctatgtgaaggttatgaattcatcatagag----ggggcgtc-taatctcactcatgtttttgttgtcaatctttgtatgtcttcagGGCTGATTGATTTGGCTATTAAGAGGAATAGTTTGATTGGTGGGGATGATTTTAAGAGTGGTCAAACCAAGATGAAATCTGTATTGGTGGATTTCCTTGT

upper sequence: GRMZM2G155242_T01 (Zea mays), 3'ss of exon 5
lower sequence: PP1S267_10V6.1 (Physcomitrella patens), 3'ss of exon 5
gtgcgtggtttggt-gtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttctgttccaaggcttat-tatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
    | |  ||  | | |  |   ||| ||| |||  ||     ||| |    ||| || |      |  ||    |        | |||| |||| | |   | |    | |||  | |||||    ||| || | || || |   | ||||| ||||| ||||||||||| ||||| |||||||||||||||||  | || || ||||| ||
----gcgacttattcgagcatctgaaaccttgatgaggtc-tgcttccg----aaaattccc-----tcataaacttaacagtgcgtttatgtatatcgggaactttggtgcagGCGTAATTGAGTACGCTGTAGAGAAGAAATCTCTTATTGGCGGTGATGACTTCAAGAGCGGACAAACCAAGATGAAATCTGTGCTTGTGGACTTCCTCGT

upper sequence: GRMZM2G155242_T01 (Zea mays), 3'ss of exon 5
lower sequence: PP1S55_204V6.1 (Physcomitrella patens), 3'ss of exon 4
gtgcgtggtttggtgtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttctgttccaaggcttattatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
|| |    || | | | ||||  | || |||   |||||||||    || ||    ||| |     ||  |   ||| | | | | |  ||  |  ||  |  || | | |||  | |||||    ||| |  |||| ||||  || ||||||||||||||||||||||| || || || ||||||||||| ||  | || || || || ||
gtacagaattggttttttttg--agaggttc---gtgttgcataagagtaccgc--ttttaaa---ttagagagctgattcgaagttggtttcaaatgcgg--tggttgcagGAGTAATTGAATACGCTGTTGAAAAGAACTCATTAATTGGTGGTGACGACTTCAAGAGCGGTCAAACGAAGATGAAATCCGTTCTAGTGGACTTTCTAGT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32838843|gb|CD978524.1|CD978524
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32925788|gb|CF030600.1|CF030600
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGCCT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32926333|gb|CF031145.1|CF031145
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|211029036|gb|FL245282.1|FL245282
EST:     CAATGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: CAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32859828|gb|CD999509.1|CD999509
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|211029043|gb|FL245289.1|FL245289
EST:     AATGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: AATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32843100|gb|CD982781.1|CD982781
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32924916|gb|CF029728.1|CF029728
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32925548|gb|CF030360.1|CF030360
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32908546|gb|CF013359.1|CF013359
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|31353688|gb|CD438045.1|CD438045
EST:     GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32863171|gb|CF002853.1|CF002853
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32925926|gb|CF030738.1|CF030738
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|211029042|gb|FL245288.1|FL245288
EST:     GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32843595|gb|CD983276.1|CD983276
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32924885|gb|CF029697.1|CF029697
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|4776520|gb|AI665523.1|AI665523
EST:     GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|6536588|gb|AW224901.1|AW224901
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGATGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT
EST: gi|32864017|gb|CF003699.1|CF003699
EST:     GGAGGGGGTGCCGTTCATCAACGGGAGCCCCCAGAACACCTTTGTGCCTG                         GGCTGATTGATCTTGCTATCAAAAACAACTGCTTGATTGGTGGTGACGACT
genomic: GGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 826

AACGAGGCGGAGGTATCACCATCAACACTATATGCCATTGCCTGTGTCATGGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 68

AACGAGGCGGAGGTATCACCATCAACACTATATGCCATTGCCTGTGTCATGGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 30

AACGAGGCGGAGGTATCACCATCAACACTATATGCCATTGCCTGTGTCATGGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 192

AACGAGGCGGAGGTATCACCATCAACACTATATGCCATTGCCTGTGTCATGGAGGGGGTGCCGTTCATCAATGGGAGCCCCCAGAACACCTTTGTGCCTGgtgcgtggtt ... atgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttcatggtgttgtatttctgttccaaggcttattatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT
                             ttattata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgcgtggtttggtgtgtttgcaaaagcttcatggtgttgcatttctgttccaaagtttcatggtgttgtatttctgttccaaggcttattatacctgttgcatgttcgtagGGCTGATTGATCTTGCTATAAAAAACAACTGCTTGATTGGTGGTGACGACTTCAAGAGTGGACAGACCAAGATGAAATCTGTCTTGGTCGATTTCCTTGT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGATG