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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtactatttctgagcttcttgtactaacttgcacaatctgtttacctttgttgctcttgttggttacgaattgctgccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG

Basic information

species Zea mays
transcript GRMZM2G135727_T03
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G135727_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os11g06750.1 (Oryza sativa), 3'ss of exon 5
----------------------------------------gtactatttctgagcttcttgtactaacttgcacaatctgtttacctttgttgctcttgttggttacgaattgctgccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG
                                         |   |  ||||   |   | |  ||   |     |||||||| |    |   ||         |     |||||        |||||| |||||||||||||||||||||||||||||||| ||||| ||| | |||||||| |||||||| ||||||||||| |||||||| || || ||||||
gtaccaattcgtctaccagatattacaccagcactccatgattgcaaatctgtcttctgtttgatatgctattgcatctgtttgcgagcgaaacttaaacgatattgtgcttgctcttgcgctcagGACTGAGAAGGACATCACTCCCATGGGTGGCTTCCCTCACTACGGTGTTGTGAAGGGCGACTACCTCATGATCAAGGGTTGCTGTGTTGGCCCCAAGAAG

upper sequence: GRMZM2G135727_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA09G00280.1 (Glycine max), 3'ss of exon 5
gtactatttctgagcttcttgtactaacttgcacaatctgtttacctttgt-tgctcttgttggttacgaattgctgcc-atttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG
    ||  | || | || ||||    | ||    |   | ||||  |  |  |||  |  ||| | |    ||| | || | |  |||||| |||||||| || ||||| |||||||| ||||| || |||||| | ||||||| ||| ||  | ||| | |||||||||||||| || || ||||||
---gtacatttgtgttttttgtttctattt---taggttatttatatcagagtgcagtatttgatcat--cttgtttcctaatgacagGACTGAGAAGGATATTACTCCTATGGGTGGGTTCCCTCATTATGGTGTGGTGAAGGATGATTATATTATGGTGAAGGGATGCTGTGTTGGACCGAAGAAG

upper sequence: GRMZM2G135727_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA20G30810.1 (Glycine max), 3'ss of exon 5
------gtactatttctga--gcttcttgtactaacttgcacaatctgt-ttacctttgttgctcttgttggttacg--aattgctgccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG
      ||  | ||  | |  | ||  || | ||| ||   ||    || ||   |||||   ||    |     |   | ||| |  |   | |||||||||||| ||||| || || || ||||| ||||| ||||||||| | || |||| ||| ||  | ||| ||||||||||||| || ||||| ||||| 
gtaagtgttttgttgattatggttttgtgcaataaatttagcaggaagtattttgtttgtaagtcaaactaacccctctacttgttttctgattcagGACCGAGAAAGACATTACCCCAATAGGTGGTTTCCCTCACTATGGTGTTGTTAAGGATGATTATATCATGGTCAAGGGATGCTGCGTTGGTCCCAAGAAA

upper sequence: GRMZM2G135727_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA10G36780.1 (Glycine max), 3'ss of exon 5
------gtactatttctga-----gcttcttgtactaacttgc---acaatctgtt-tacctttgttgc---tcttgttggtta-cgaattgctgccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG
      ||  | ||  |||     | ||  || | ||| ||     |||    |   ||| || ||||    ||   ||      | | ||| |  |   | |||||||||||| ||||| || || |||||||| ||||| ||||||||| | || |||| ||| |   | ||| |||||||||||||||| ||||| ||||||
gttagtgttgtcttcttgattatggttttgtgcagtaaattattgaacagcaggaagtactttggttgtaagtcaaattaaccctctacttgttttctgatccagGACCGAGAAAGACATTACCCCAATGGGTGGTTTCCCTCACTATGGTGTTGTTAAGGATGATTTTATCATGGTCAAGGGATGCTGTGTTGGTCCCAAGAAG

upper sequence: GRMZM2G135727_T01 (Zea mays), 3'ss of exon 5
lower sequence: AT1G61580.1 (Arabidopsis thaliana), 3'ss of exon 5
------gtactatt--tctgagcttcttgtactaacttgcacaatctgtttacctttgttgctcttgttg-gttacgaattgctgccattt--gcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG
       |||||    |||     | ||  | | |  ||   | |  |   || | | ||| | ||  |  | |      || |  | |||    || ||||||||||| ||||| || ||||| || || ||||||||||| || |||||||  || ||  |||||||||||||||| || ||||| || ||||| 
gtaataatactaacgatcttgaggtgtttcagtcatctgtgtagtaagcggacttgtcttggtgttcatatgctgttttctgttttcttttcaatagAACCGAGAAGGATATCACACCAATGGGAGGATTTCCCCACTATGGGATAGTGAAGGAAGATTATTTGATGATCAAGGGATGTTGCGTGGGGCCTAAGAAA

upper sequence: GRMZM2G135727_T01 (Zea mays), 3'ss of exon 5
lower sequence: Vv18s0072g00340.t01 (Vitis vinifera), 3'ss of exon 5
------gtactatttctgagcttct-tgtactaacttgca------caatctgtttacctttgttgctcttgttggttacgaattgctgccatttg-cagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG
       || ||   ||  | || | |   | | ||  ||      |||  |   |||| |   |  | |  ||| ||    | |      ||    |||||||||||| ||||| ||||| |||||||| ||||| ||||| ||| | ||||||| ||| ||  ||||||||||||| |||||||| || || ||||||
taagtaataatagccctatgtttgtatactcaatctcacaggatgccaacattgctaccgtaactaatatcattgtttctttacttggataataaaacagGACCGAGAAAGACATAACTCCAATGGGTGGTTTCCCTCACTACGGTGTGGTGAAGGATGATTATGTGATGATCAAGGGTTGCTGTGTTGGGCCTAAGAAG

upper sequence: GRMZM2G135727_T01 (Zea mays), 3'ss of exon 5
lower sequence: EDP06420 (Chlamydomonas reinhardtii), 3'ss of exon 5
----------------------------------------------------------------------------gtactatttctgagcttct-tgtactaacttgcacaatctgtttacctttgttgctcttgttggttacgaattgctgccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACT--ATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG----------------------------
                                                                            || ||    | | |  ||  ||   | | |    |  | |  |  |          ||| |   || |     |   ||  |||   |     |  |  |||   | |   | |  | |   |    |       |  |   | |    | |||||   | || ||  | |  ||||      ||| ||                            
gtgcgaggcggccgtggcgactggaataatgtttagtgccctgttggggagggtgtaccggattatggggatggctgtgctgccgcagcgtatcggtgggatgattcctgcgttgtcctcgctccacggctccttttccattgcttgcggtcaccgcttgtgcgcatctgccgtgcattcatgcttggcgcagGTGTACAAGATCGGCAAGAAGGGCGACGCCAGCCACCTGGCCACCACGGAGTTCGATGTGACCAAGAAGGAGATCACCCCCATGGGTGGCTTCCCCCACT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67020267|gb|CO449016.1|CO449016
EST:     ATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAG                         GACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGT
genomic: ATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAGgtactatttc ... ccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGT
EST: gi|67024182|gb|CO452931.1|CO452931
EST:     ATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAG                         GACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGT
genomic: ATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAGgtactatttc ... ccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGT
EST: gi|32916546|gb|CF021358.1|CF021358
EST:     ATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAG                         GACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGG
genomic: ATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAGgtactatttc ... ccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 1680

GTCAGAATGGGTACCACCACCGCACTGAGATGAACAAGAAGGTCTACAAGATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAGgtactatttc ... ccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG


Block sizes: 49 (upstream exon), 43 (downstream exon)
Score: 1426

GTCAGAATGGGTACCACCACCGCACTGAGATGAACAAGAAGGTCTACAAGATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAGgtactatttc ... ccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG


Block sizes: 49 (upstream exon), 36 (downstream exon)
Score: 1093

GTCAGAATGGGTACCACCACCGCACTGAGATGAACAAGAAGGTCTACAAGATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAGgtactatttc ... ccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG


Block sizes: 49 (upstream exon), 42 (downstream exon)
Score: 540

GTCAGAATGGGTACCACCACCGCACTGAGATGAACAAGAAGGTCTACAAGATCGGCAAGGCTGGACAAGAGACCCACGATGCCTCCACAGAGTTTGACAGgtactatttc ... ccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtactatttctgagcttcttgtactaacttgcacaatctgtttacctttgttgctcttgttggttacgaattgctgccatttgcagGACCGAGAAGGACATCACTCCCATGGGTGGCTTCCCCCACTATGGTATCGTGAAGGGTGACTACCTGATGATCAAGGGATGCTGTGTGGGTCCAAAGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ATGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG