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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtcagattcaaatgtcgagcacgccatttgtctccaacgagacaacgatgtagatgcttagactctttgatgtgccatgttgtgcacatttgaaccttatcaattcttcctcatatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCAGCAAAGCAGGAGGACATGGTGGATGTCAACCTGAAGTCGACTTCTAGCC

Basic information

species Zea mays
transcript GRMZM2G128771_T01
intron # 5
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149095335|gb|EE180972.2|EE180972
EST:     TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAG                         CCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
genomic: TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
EST: gi|37387848|gb|CF631126.1|CF631126
EST:     TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAG                         CCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
genomic: TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
EST: gi|211492535|gb|FL462462.1|FL462462
EST:     TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAG                         CCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
genomic: TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
EST: gi|211435904|gb|FL357606.1|FL357606
EST:     TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAG                         CCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
genomic: TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
EST: gi|37379915|gb|CF626729.1|CF626729
EST:     TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAG                         CCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
genomic: TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
EST: gi|40335969|gb|CK370039.1|CK370039
EST:     TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAG                         CCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
genomic: TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
EST: gi|211481374|gb|FL368109.1|FL368109
EST:     TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCTAG                         CCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
genomic: TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
EST: gi|211492534|gb|FL462461.1|FL462461
EST:     TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAG                         CCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA
genomic: TGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 28 (upstream exon), 43 (downstream exon)
Score: 4

ACAAGTGTCCACAGAAGAAGGAGAGAGCAAGGCAAAAGAATTGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCAGCAAAGCAGGAGGACATGGTGGATGTCAACCTGAAGTCGACTTCTAGCC


Block sizes: 28 (upstream exon), 26 (downstream exon)
Score: 7

ACAAGTGTCCACAGAAGAAGGAGAGAGCAAGGCAAAAGAATTGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCAGCAAAGCAGGAGGACATGGTGGATGTCAACCTGAAGTCGACTTCTAGCC


Block sizes: 28 (upstream exon), 49 (downstream exon)
Score: 5

ACAAGTGTCCACAGAAGAAGGAGAGAGCAAGGCAAAAGAATTGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCAGCAAAGCAGGAGGACATGGTGGATGTCAACCTGAAGTCGACTTCTAGCC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 100

ACAAGTGTCCACAGAAGAAGGAGAGAGCAAGGCAAAAGAATTGAATGTCATGTTCATCGAGACCAGCGCGAAAGCCGGCTTCAACATCAAGgtcagattca ... atatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCAGCAAAGCAGGAGGACATGGTGGATGTCAACCTGAAGTCGACTTCTAGCC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgatgtgccatgttgtgcacatttgaaccttatcaattcttcctcatatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCAGCAAAGCAGGAGGACATGGTGGATGTCAACCTGAAGTCGACTTCTAGCC
 ctttgat  putative branch site (score: 100)
                                    ttcttcctcat  putative PPT
                             ttatcaatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtcagattcaaatgtcgagcacgccatttgtctccaacgagacaacgatgtagatgcttagactctttgatgtgccatgttgtgcacatttgaaccttatcaattcttcctcatatgtgtagCCGTTGTTCCGCAAGATCGCTGCAGCCCTGCCAGGAATGGAGACATTGTCAGCAAAGCAGGAGGACATGGTGGATGTCAACCTGAAGTCGACTTCTAGCC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGAAG