ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

...tacacgtcagtaaactattttctacctgctcatttgaaccagagttcggtgcgtaaggttttgtgctttgtcttattctgatttcataccttgcgtgcagATCTAGGATTGCAGTGTGGCAGATAGGCCTAACATTACAACATGGTGTTCTTTACAGAATGGACAGCATAGTTTATGTATTTCTTCAATTTTGTTGATTA

Basic information

species	Zea mays
transcript	GRMZM2G124502_T02
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G124502_T02 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os04g56980.1 (Oryza sativa), 3'ss of exon 5

tacacgtcagtaaactattttctacctgctcatttga-accagagttcggtgcg-taaggttttgtgctttgtcttattctgatttcatacct-tgcgtgcagATCTAGGATTGCAGTG-TGGCAGATAGGCCTAACATTACAACATGGTGTTCTTTACAGA---ATGGACAGCATAGTTTATGTATTTCTTCAATTTTGTTGATTA
     | || |   |  | |  | |  |  |||  || |      |    |  | |||  |  | || ||||| |  ||||| | |  ||||| | | ||||||||||||||||||||| || |||||||||||||| |||||| ||||||||||| ||| |   ||||||||| ||| |||||||| | ||| ||  | | ||   
--tttgacattcc-cagtatgatgctagtacatcagagatttatctattatctgctaaattgatttgatttgtttcgttctgttctgttacctgtccatgcagATCTAGGATTGCAGTGGTGCCAGATAGGCCTAACTTTACAATATGGTGTTCTTGACAAAAAAATGGACAGC-TAGCTTATGTATCTTTTCTATGCTCTGGA---

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|71440369|gb|DR821419.1|DR821419

EST:     ATGCTTGGTCACCACTTCATCAAGAAAACATAGCAAATCCTCATTAACTG                         ATCTAGGATTGCAGTGTGGCAGATAGGCCTAACATTACAACATGGTGTTCT
genomic: ATGCTTGGTCACCACTTCATCAAGAAAACATAGCAAATCCTCATTAACTGgtcagtttct ... ttgcgtgcagATCTAGGATTGCAGTGTGGCAGATAGGCCTAACATTACAACATGGTGTTCT

EST: gi|74239585|gb|DT647499.1|DT647499

EST:     ATGCTTGGTCCCCACTTCATCAAGAAAACATAGCAAATCCTCATTAACTG                         ATCTAGGATTGCAGTGTGGCAGATAGGCCTAACATTCCAACATGGTGTTCT
genomic: ATGCTTGGTCACCACTTCATCAAGAAAACATAGCAAATCCTCATTAACTGgtcagtttct ... ttgcgtgcagATCTAGGATTGCAGTGTGGCAGATAGGCCTAACATTACAACATGGTGTTCT

EST: gi|71422217|gb|DR805651.1|DR805651

EST:     ATGCTTGGTCCCCCCTTCATCAAGAAAACATAGCAAATCCTCATTAACTG                         ATCTAGGATTGCAGTGTGGCAGATAGGCCTACCATTACACCATGGTGTTCT
genomic: ATGCTTGGTCACCACTTCATCAAGAAAACATAGCAAATCCTCATTAACTGgtcagtttct ... ttgcgtgcagATCTAGGATTGCAGTGTGGCAGATAGGCCTAACATTACAACATGGTGTTCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 43 (upstream exon), 47 (downstream exon)
Score: 73

TTCAAGAGCTCTTTGGCTGTGAGAGCATTCCTATGTCAGGACTATCAGAAATGCTTGGTCACCACTTCATCAAGAAAACATAGCAAATCCTCATTAACTGgtcagtttct ... ttgcgtgcagATCTAGGATTGCAGTGTGGCAGATAGGCCTAACATTACAACATGGTGTTCTTTACAGAATGGACAGCATAGTTTATGTATTTCTTCAATTTTGTTGATTA

Block sizes: 47 (upstream exon), 47 (downstream exon)
Score: 44

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 78

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 30

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tcggtgcgtaaggttttgtgctttgtcttattctgatttcataccttgcgtgcagATCTAGGATTGCAGTGTGGCAGATAGGCCTAACATTACAACATGGTGTTCTTTACAGAATGGACAGCATAGTTTATGTATTTCTTCAATTTTGTTGATTA