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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...cgtcgaacctatgacctttggctcggtgtgaaggcttcccacctccatgccaagcctgtctttattggcttcttattcatgataataatttggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTTTGTACCGTCTTGGGGATGGATTTTCTCAGCACAGTGATTGAAGTTCATC

Basic information

species Zea mays
transcript GRMZM2G101874_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G101874_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os06g20370.1 (Oryza sativa), 3'ss of exon 4
cgtcgaacctatgacctttg-gctcggtgtgaaggcttcccacctccatgccaagcctgtctttattggcttcttattcatgataataatttggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTTTGTACCGTCTTGGGGATGGATTTTCTCAGCACAGTGATTGAAGTTCATC
    | || | | |||| |    |   |      | | |   |    |||||   ||  |  | |   | ||    | | |  |   |||| ||||||||||| |||||||||||||||||||||||||||||  ||||  ||||| |||| ||||| ||||| |||||||||||||| |||||||||| |||||||||||
----gtacatgtaacctgtataatttcttcatttggtgctagctgtgatgcctgaccattgctgacaagatttggttccct--tgggaatt-ggtttgcagAGCAATAGGTTGGAGAAGGTTCTTGATTATGTGAGCATGATACATAATCTCTGTACTGTCTTAGGGATGGATTTTCTTAGCACAGTGACTGAAGTTCATC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211376680|gb|FL281484.1|FL281484
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|148946917|gb|EC892199.2|EC892199
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|211374024|gb|FL281477.1|FL281477
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|26456561|gb|CA828144.1|CA828144
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|60342722|gb|DN209695.1|DN209695
EST:     AGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|211374011|gb|FL281464.1|FL281464
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|149058039|gb|EE156723.2|EE156723
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|211374012|gb|FL281465.1|FL281465
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|211376675|gb|FL281479.1|FL281479
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|211376676|gb|FL281480.1|FL281480
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|211374019|gb|FL281472.1|FL281472
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|211374017|gb|FL281470.1|FL281470
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|91875287|gb|EB405244.1|EB405244
EST:     AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
EST: gi|211376678|gb|FL281482.1|FL281482
EST:     AGAGGCTTGAAGATTTTCGGNCTCAGCTCAAGGATCTTGAAAAAGAGAAG                         AGTAATAGGNTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT
genomic: AGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 358

GGGTGAGCAAATGACAACACCCCAAGTCAATGAGGATGACTTAACACTTGAGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTTTGTACCGTCTTGGGGATGGATTTTCTCAGCACAGTGATTGAAGTTCATC


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 63

GGGTGAGCAAATGACAACACCCCAAGTCAATGAGGATGACTTAACACTTGAGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTTTGTACCGTCTTGGGGATGGATTTTCTCAGCACAGTGATTGAAGTTCATC


Block sizes: 48 (upstream exon), 45 (downstream exon)
Score: 42

GGGTGAGCAAATGACAACACCCCAAGTCAATGAGGATGACTTAACACTTGAGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTTTGTACCGTCTTGGGGATGGATTTTCTCAGCACAGTGATTGAAGTTCATC


Block sizes: 46 (upstream exon), 47 (downstream exon)
Score: 80

GGGTGAGCAAATGACAACACCCCAAGTCAATGAGGATGACTTAACACTTGAGAGGCTTGAAGATTTTCGGTCTCAGCTCAAGGATCTTGAAAAAGAGAAGgtacttttct ... tggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTTTGTACCGTCTTGGGGATGGATTTTCTCAGCACAGTGATTGAAGTTCATC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

catgccaagcctgtctttattggcttcttattcatgataataatttggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTTTGTACCGTCTTGGGGATGGATTTTCTCAGCACAGTGATTGAAGTTCATC
                        ttcttattcatgataa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
cgtcgaacctatgacctttggctcggtgtgaaggcttcccacctccatgccaagcctgtctttattggcttcttattcatgataataatttggtttgcagAGTAATAGGTTGGAGAAGGTTCTTGATTATGTAGGCATAGTACATGATCTTTGTACCGTCTTGGGGATGGATTTTCTCAGCACAGTGATTGAAGTTCATC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GATGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG
- - - - - - - - - - - - - - - - -gcttccc
- - - - - - - - - - - - - - - - - - - - - -tccatgc
- - - - - - - - - - - - - - - - - - - - - - - - - caagcct