ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtaagtctgttgatcacatcatgcaatctctctctctctctctctctgctaacattgcttatttatgttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGCTGCTTACAGCTTCTTGGTTTCTGTACCTTTGAGTCATGTGTTGGTATAT

Basic information

species	Zea mays
transcript	GRMZM2G067638_T01
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G067638_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os10g37520.1 (Oryza sativa), 3'ss of exon 5

gtaagtctgttgatcacatcatgcaatct---ctctctctctctctctctg----ctaacattgcttatttatgttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGCTGCTTACAGCTTCTTGGTTTCTGTACCTTTGAGTCATGTGTTGGTATAT
||||||||| ||  | ||   |    ||     | || || | |   ||       |||||     | ||| ||||  | | ||||||||||||||||||||  ||  ||||| ||  |||| || || ||||||||  ||||||| || ||||||||||  ||||| || |||||||| || |
gtaagtctgctgcactcagtttattctcaatgttttcactttatagttcgaaagtttaacacaatctgtttttgtt--aaatagTTCCTAGTACCTCCCTCCTCGGTCAAAGGTGGTGGCATCTCATTTGGAGGCTGTCTACAGCTGCTCGGTTTCTGTATATTTGAATCCTGTGTTGGCATTT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|86473298|gb|DY239668.1|DY239668

EST:     CTCTTTTCCTTCCCCGTTGTCACCAAT                         TTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC
genomic: CTCTTTTCC-TCCCCGTTGTCACCAATgtaagtctgt ... ttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC

EST: gi|20208264|gb|BQ134353.1|BQ134353

EST:     TCGTGTTCTCAGTATCAGCCTTTACTCTTTTCCTCCCTGTTGTCACCAAT                         TTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC
genomic: TCGTGTTTTCAGTATCAGCCTTTACTCTTTTCCTCCCCGTTGTCACCAATgtaagtctgt ... ttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC

EST: gi|74246169|gb|DT654083.1|DT654083

EST:     TCGTGTTTTCAGTATCAGCCTTTACTCTTTTCCTCCCCGTTGTCACCAAT                         TTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC
genomic: TCGTGTTTTCAGTATCAGCCTTTACTCTTTTCCTCCCCGTTGTCACCAATgtaagtctgt ... ttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC

EST: gi|26456031|gb|CA827614.1|CA827614

EST:     TCG-GTTTTCAGTATCAGCCTTTACTCTTTTCCTCCCTGTTGTCACCAAT                         TTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC
genomic: TCGTGTTTTCAGTATCAGCCTTTACTCTTTTCCTCCCCGTTGTCACCAATgtaagtctgt ... ttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC

EST: gi|78117635|gb|DV536022.1|DV536022

EST:     CAGCCTTTACTC-TTTCCTCCCCGTTGTCACCAAT                         TTCNTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC
genomic: CAGCCTTTACTCTTTTCCTCCCCGTTGTCACCAATgtaagtctgt ... ttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 60

TGCTTCTCGTTTGTTAGCTAGGAAGCTGAAGGTTGAAGGTTATATGCAAATCGTGTTTTCAGTATCAGCCTTTACTCTTTTCCTCCCCGTTGTCACCAATgtaagtctgt ... ttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGCTGCTTACAGCTTCTTGGTTTCTGTACCTTTGAGTCATGTGTTGGTATAT

Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 44

Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 26

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 113

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

gcaatctctctctctctctctctctgctaacattgcttatttatgttttatacagTTCCTAGTACCTCCCTCCGGGGAGAAAGGCGGCAGCATTTCGTTAGGAGGCTGCTTACAGCTTCTTGGTTTCTGTACCTTTGAGTCATGTGTTGGTATAT