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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ataaaaattcaaattcacttcctcttgcaacacatggattcttttagaaatatgccattatgctcatcgatctcatgacaatatccttttccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC

Basic information

species Zea mays
transcript GRMZM2G058276_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G058276_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os03g42220.1 (Oryza sativa), 3'ss of exon 6
ataaaaattcaaattcacttcctcttgcaacacatggattcttttagaaatatgccattatgctcat--cgatctcat--gacaatatccttttccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC
 || || |||||    |  |||   ||||  |  |   || | | |  |||||||     ||  |||   ||||| |   || ||  | | |||   |||||||||||||| ||| | ||||||||||| ||||| |||||||| |||||||||||||| || || || |||||||||||||||||||||||||| ||||||||
gtataagttcaa--cgaactccatatgcattatttc--ttgtctaaagaatatgctcacctgaacatgttgatctgactggataagcttcctttggatctgcagATAAGTTTAGATCAGATCTCATGAACATTGCCATGACTACACTTAGTTCAAAAATCCTCTCTCAGGACAAGGAGTACTTTGCTGAACTTGCTGTTGATGC

upper sequence: GRMZM2G058276_T01 (Zea mays), 3'ss of exon 5
lower sequence: Vv18s0001g00480.t01 (Vitis vinifera), 3'ss of exon 7
ataaaaattcaaattcacttcctcttgcaacacatggattcttttagaaatatgccatta-tgctcatcgatctcatgacaatatccttttccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC
 |||  ||     | ||     | ||     | |    | | | ||  | | | || | | |  | ||  |||||||||  |  |  | |   |  |||||| |||||||     ||  | ||||| ||||| ||||||||| | ||||||||||| |||||||| ||||||||  | |||||  |||| || || |||||
ctaaccatgttggt-catgatatattatgtaatacttttccatctaatatttttccttaaattattatatatctcatgaataatttttctggttaatgcagAAAAGTTCAAGTTAGACTTGATGAAGATTGCAATGACTACTTTGAGTTCAAAAATTCTTTCCCAGGACAAGGAACATTTTGCAAAACTGGCAGTAGATGC

upper sequence: GRMZM2G058276_T01 (Zea mays), 3'ss of exon 5
lower sequence: EFJ28920 (Selaginella moellendorffii), 3'ss of exon 6
ataaaaattcaaattcacttcctcttgcaacacatggattcttttagaaatatgccattatgctcatcgatctcatgacaatatccttttccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC
                                       ||| || | | |    ||  ||| | |  || ||||      | |  || ||| | |||||| |||||  ||   ||||| || |||||||||||||| ||||| ||||||||||| ||||| || || || ||  | |||||  | ||||| ||  ||||
--------------------------------------gtctgttggcactgcttca--atgttta--gagctca--gtcttgtgtttgtccat-tgcagAAAAGTTTCGACAAGATCTTATCAATATTGCTATGACAACTCTGAGTTCAAAAATTCTTTCTCAGGAGAAAGAACATTTTGCCAATCTTGCTGTGAATGC

upper sequence: GRMZM2G058276_T01 (Zea mays), 3'ss of exon 5
lower sequence: EFJ13624 (Selaginella moellendorffii), 3'ss of exon 6
ataaaaattcaaattcacttcctcttgcaacacatggattcttttagaaatatgccattatgctcatcgatctcatgacaatatccttttccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC
                                       ||| || | | |    ||      |  | || ||||      | |  || ||| | |||||| |||||  ||   ||||| || |||||||||||||| ||||| ||||||||||| ||||| || || || ||  | |||||  | ||||| ||  ||||
--------------------------------------gtctgttggcactgcttca----atttttagagctca--gtcttgtgtttgtccat-tgcagAAAAGTTTCGACAAGATCTTATCAATATTGCTATGACAACTCTAAGTTCAAAAATTCTTTCTCAGGAGAAAGAACATTTTGCCAATCTTGCTGTGAATGC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211242377|gb|FL471585.1|FL471585
EST:     TGCCAGAAATGCTTTGTTACAAAGGACCATGGACAACAAAGAAAATACAG                         ATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
genomic: TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
EST: gi|211486980|gb|FL134661.1|FL134661
EST:     TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAG                         ATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
genomic: TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
EST: gi|211335042|gb|FL425887.1|FL425887
EST:     TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAG                         ATAAGTTCAGAGCTGATCTCATGAATAATGCTATGACTACTCTTAGTTCAA
genomic: TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
EST: gi|60400200|gb|DN233007.1|DN233007
EST:     TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAG                         ATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
genomic: TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
EST: gi|32909064|gb|CF013876.1|CF013876
EST:     GCCAGAAATGGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAG                         ATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
genomic: GCCAGAAATG-CTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
EST: gi|211346060|gb|FL392059.1|FL392059
EST:     TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAG                         ATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA
genomic: TGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 158

GTTACAGAATGGCTCTCGAGTGTGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 87

GTTACAGAATGGCTCTCGAGTGTGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 72

GTTACAGAATGGCTCTCGAGTGTGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 71

GTTACAGAATGGCTCTCGAGTGTGCCAGAAATGCTTTGCTACAAAATACCATGGACAACAAAGAAAATACAGgtaaattatt ... ccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agaaatatgccattatgctcatcgatctcatgacaatatccttttccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC
                                    tatccttttccttctg  CT-rich tract
                                acaatat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ataaaaattcaaattcacttcctcttgcaacacatggattcttttagaaatatgccattatgctcatcgatctcatgacaatatccttttccttctgcagATAAGTTCAGAGCTGATCTCATGAATATTGCTATGACTACTCTTAGTTCAAAAATACTTTCCCAAGACAAGGAGTACTTTGCTGAACTTGCGGTTGATGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAG
ataaaaa
- - - - - - - - - - - tcttgca
- - - - - - - - - - - - - - - cacatgg
- - - - - - - - - - - - - - - - - - - - - - - - - tatgcca
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - tatgctc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -catgaca