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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtccgtttcttgaccacccaccctgacaaggttcttgaggtgctttatgctttgattgaatgaattgaatcaaccatggttcagaggttgtgacatctgatcgaatcctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTGCAGCCGGTGCAGGAGGCGAGCACGAGGAGCAGCAGCAGGATGGGGACGG

Basic information

species Zea mays
transcript GRMZM2G044004_T03
intron # 5
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71298151|gb|DR785052.1|DR785052
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
EST: gi|211185322|gb|FL304176.1|FL304176
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
EST: gi|71440982|gb|DR822032.1|DR822032
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
EST: gi|50328106|gb|CO523232.1|CO523232
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
EST: gi|211185318|gb|FL304172.1|FL304172
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
EST: gi|211185320|gb|FL304174.1|FL304174
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
EST: gi|91048835|gb|EB159253.1|EB159253
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
EST: gi|211185319|gb|FL304173.1|FL304173
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
EST: gi|211185321|gb|FL304175.1|FL304175
EST:     CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAG                         ATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG
genomic: CGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 142

ACCACCACTCTGGGGCAAGGCGACGACGGCTGCAAAGGCCGCAGCGCCAACGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTGCAGCCGGTGCAGGAGGCGAGCACGAGGAGCAGCAGCAGGATGGGGACGG


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 34

ACCACCACTCTGGGGCAAGGCGACGACGGCTGCAAAGGCCGCAGCGCCAACGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTGCAGCCGGTGCAGGAGGCGAGCACGAGGAGCAGCAGCAGGATGGGGACGG


Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 37

ACCACCACTCTGGGGCAAGGCGACGACGGCTGCAAAGGCCGCAGCGCCAACGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTGCAGCCGGTGCAGGAGGCGAGCACGAGGAGCAGCAGCAGGATGGGGACGG


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 29

ACCACCACTCTGGGGCAAGGCGACGACGGCTGCAAAGGCCGCAGCGCCAACGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGgtccgtttct ... cctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTGCAGCCGGTGCAGGAGGCGAGCACGAGGAGCAGCAGCAGGATGGGGACGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gaattgaatcaaccatggttcagaggttgtgacatctgatcgaatcctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTGCAGCCGGTGCAGGAGGCGAGCACGAGGAGCAGCAGCAGGATGGGGACGG
                                 atctgat  putative branch site (score: 29)
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtccgtttcttgaccacccaccctgacaaggttcttgaggtgctttatgctttgattgaatgaattgaatcaaccatggttcagaggttgtgacatctgatcgaatcctgtagcagATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTGCAGCCGGTGCAGGAGGCGAGCACGAGGAGCAGCAGCAGGATGGGGACGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGGATG