ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...taacagaaaacatttgctttctgctggtttgtttacgttatttattcatcaatgcaatacttgatttatacaagcaaccctaatttaaaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG

Basic information

species	Zea mays
transcript	GRMZM2G034143_T01
intron #	5
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G034143_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os02g17320.1 (Oryza sativa), 3'ss of exon 5

taacagaaaacatttgctttctgctggtttgtttacgttatttattcatcaatgcaatacttgatttatacaagcaaccctaat-ttaaaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG
 || |    |    || || | | |  ||  | | | || ||||| |||   ||   ||||| || | |   || ||||||||| ||   |||   | ||||| |||||||||||||| |||||||||||||||||||| ||||| |||||||||||  || ||||||||||||||||||||||||| ||||| ||||
gaaaattggatcactgattacagtttattcttct-ctttgtttatccatatctgttttactttatgtttcagagaaaccctaatattttgacttctgtcagGTCAATCCTTCAGATGTAATTATTTTGGAAGGAATTCTGGTTTTCCATGATTCACGTGTCCGGGATTTGATGAACATGAAGATCTTTGTTGACACAG

upper sequence: GRMZM2G034143_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os11g16370.1 (Oryza sativa), 3'ss of exon 5

--taacagaaaacatttgctttctgctggtttgtttacgttatttattcatcaatgcaatacttgatttatacaagcaaccctaat-ttaaaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG
   || |  | |    |||||   | | |||  || || ||||||||||||   ||   ||||| || | |   ||  |||||||| ||   ||||  | ||||| |||||||||||||| |||||||||||||| ||||| ||||| |||||||||||  || |||| ||||||||||||||||| || ||||||||||
aagaaaattagatcactgcttatagtttgttc-ttcactttatttattcatatttgttttactttatgtttcagag--accctaatattttgactgctgtcagGTCAATCCTTCAGATGTAATTATTTTGGAAGGTATTCTGGTTTTCCATGATTCACGTGTCCGGGACTTGATGAACATGAAGATATTTGTTGATACAG

upper sequence: GRMZM2G034143_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA18G08180.1 (Glycine max), 3'ss of exon 5

taacagaaaacatttgctttctgctggtttgtttacgttatttattcatcaatgcaatacttgatttatacaagcaaccctaatttaaaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG-
 | || | ||   |    |||     |  | |||  | | |   || || ||     |       | ||   |       |   || |   |||   ||||| || |||||||||||||| ||||| ||||| || || ||||| |||||| ||||  |  |||| |||||||| |||||||| || ||||| |||| 
aagcatagaatcatgatattccaaatgcatattttagct-tagttttatgaagttgctgtcattctcatttcattttgtgttgctttatcatgttttcaggTGAACCCTTCAGATGTTATAATTTTAGAAGGCATCCTTGTTTTCCATGATCCACGTGTTCGGGAGTTGATGAATATGAAGATATTTGTTGACACAGG

upper sequence: GRMZM2G034143_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA02G47270.1 (Glycine max), 3'ss of exon 5

taacagaaaacatttgctttctgctggtttgtttacgtta---tttattcatcaatgcaatac---ttgatttatacaagca----accctaatttaaaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG
          ||| |   ||||   | ||| | | | |||   |  |    |  ||| |||||   || |  |||||   |     |   |  |||||     |   ||||| || ||  | |||||||||||||||||||| || || |||||||||||| ||||  |  | |  |||||||| |||||||| || ||||| ||||
----------catgtttcttcttgcgttttatctccattagagtggaaatttatatggaatacaatttcagctatacttcccttttatgttggtttaatctgttttgcagGTAAACCCAGCGGATGTTATTATTTTGGAAGGTATCCTTGTTTTTCATGATCCACGAGTGCGAGCATTGATGAATATGAAGATATTTGTTGACACAG

upper sequence: GRMZM2G034143_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA08G45270.1 (Glycine max), 3'ss of exon 5

taacagaaaacatttgctttctgctggtttgtttacgttatttattcatcaatgcaatacttgatttatacaagcaaccctaattta----aaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG
  | | || ||||||  |   || | ||     | | | |||| |   |  |   | |  |||   |   ||    |   |   ||     |   |||   ||||| || |||||||||||||| ||||||||||| || || ||||| |||||  ||||  |  |||| |||||||| |||||||| || ||||| ||||
--ataaaatacattt--tacatgatagtccaaatgcataatttttagttttacaaagttgttgtcattctcatttcattttgtgttgccttatcgtgttttcagGTGAACCCTTCAGATGTTATAATTTTGGAAGGCATCCTTGTTTTCCATGACCCACGTGTTCGGGAGTTGATGAATATGAAGATATTTGTTGACACAG

upper sequence: GRMZM2G034143_T01 (Zea mays), 3'ss of exon 5
lower sequence: Vv19s0015g01730.t01 (Vitis vinifera), 3'ss of exon 5

taacagaaaacatttgctttctgctggtttgtttacgttatttattcatcaatgcaatacttgatttat--acaagcaaccctaatttaaaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG
  |  |    | |      | ||    | | |||  | || ||  |      | |     |  ||  ||  | || |  |  |  | | |||   | | ||||| || ||||||||||| || ||||||||||| || ||  |||||||||||   ||  || |||| ||||||||||||||||| || ||||||||||
--agggtggtcctgaatcctgtgagattatatttcaggtacttgatttctgttcccgggttacatgcattgagaaccctcattgctatgaaatatttggcagGTCAACCCTTCAGATGTGATAATTTTGGAAGGCATACTCATTTTTCATGATCTTCGTGTCCGGGAATTGATGAACATGAAGATATTTGTTGATACAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|78113249|gb|DV531643.1|DV531643

EST:     TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAG                         GTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT
genomic: TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT

EST: gi|71439660|gb|DR820710.1|DR820710

EST:     TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAG                         GTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT
genomic: TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT

EST: gi|148995152|gb|EE034432.2|EE034432

EST:     TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAG                         GTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT
genomic: TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT

EST: gi|18661324|gb|BM501354.1|BM501354

EST:     TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAG                         GTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT
genomic: TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT

EST: gi|31359325|gb|CD443682.1|CD443682

EST:     TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAG                         GTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT
genomic: TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT

EST: gi|149020749|gb|EE045750.2|EE045750

EST:     TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAG                         GTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT
genomic: TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT

EST: gi|148943394|gb|EC888453.2|EC888453

EST:     TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAG                         GTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT
genomic: TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT

EST: gi|149088610|gb|EE177727.2|EE177727

EST:     TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAG                         GTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT
genomic: TCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGAT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 101

GCTACTTCTCTCTTGTATGCAAAACTTGAAACATGGCAAAGCTGTCGACATCCCTAATTACAACTTCAAGACATACAAGAGTGTACCAAATGCAAGGAAGgtaatatttt ... ctgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG

Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 72

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 62

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 79

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tcatcaatgcaatacttgatttatacaagcaaccctaatttaaaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG

                                ccctaat  putative branch site (score: 79)
          aatacttgatttata  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
taacagaaaacatttgctttctgctggtttgtttacgttatttattcatcaatgcaatacttgatttatacaagcaaccctaatttaaaactgtcgccagGTTAATCCTTCAGATGTTATTATTTTGGAAGGAATTCTAGTTTTTCATGATTCACGCCTCAGGGATTTGATGAACATGAAGATCTTCGTTGATACAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
-aacagaa
- - - - - - tttgctt
- - - - - - - - - -tctgctg
- - - - - - - - - - - - - - - - - - - - - - - - -caatgca
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - caagcaa