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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttaagtacaatagtagatttgtttatggtagcattctggttctcatgaattaagagacagttttgattttattccacaaattatgtattgaactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG

Basic information

species Zea mays
transcript GRMZM2G017089_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G017089_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os03g21990.1 (Oryza sativa), 3'ss of exon 7
ttaagtacaatagtagatttgtttatggtagcattctggttctcatgaattaagagacagttttgatt--ttattccacaaattatgtattgaactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG
            |||  ||| |      |||    |||| | |  |   ||     |   |||  | |  |  || | |||| | |||          |||||| ||||||||||||||||||||||| || || |||||||||||||||   || ||||||||||| || ||||||||||| ||||| || |||
------------gtaactttttcctcattagtg--ctggattttctagttttgcctatgatttgcactaatggtttctcaaaatgtgt----------gcagGTGATCAGTGGTCACTTGGGATGGGTGCGGTCAATTGCATTTGATCCAAGTAACGAATGGTTCTGTACGGGTTCTGCCGACAGGACGATTAAG

upper sequence: GRMZM2G017089_T01 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA09G04910.1 (Glycine max), 3'ss of exon 6
-ttaagtacaatagtagatttgtttatggtagcattctggttctcatgaattaagagacagttttgattttattccacaaattatgtattgaactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG
  | ||| |     ||  |   |||   |  || || |   |   |  | ||  || | ||||||  ||   |      ||   ||    |      | |||||||||| |||||||||||||||||  ||||| ||||| |||||||    ||   |||||| || ||||||||||| ||| | || || |||
aatgagttctgacctatgta-attttccgaggccttttaaattgtaatacttttgatatagttttagttgagtgatttgaaccttgataagtttgttgtagGTCATCAGCGGTCACTTGGGATGGGTGAGATCTGTTGCAGTTGATCCCAGTAACACATGGTTTTGTACTGGTTCTGCAGATCGAACTATCAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|78085590|gb|DV513983.1|DV513983
EST:     CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGG                         GTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
genomic: CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
EST: gi|78087205|gb|DV515598.1|DV515598
EST:     CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGG                         GTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
genomic: CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
EST: gi|6021220|gb|AW066148.1|AW066148
EST:     CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGG                         GTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
genomic: CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
EST: gi|32933221|gb|CF038033.1|CF038033
EST:     CTAGTAGATGGCCGCGGCCTACATGGCATGCTCCTTGGAAGAACTATCGG                         GTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
genomic: CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
EST: gi|148951460|gb|EC897185.2|EC897185
EST:     CTAGTAGAAGGGCACGGCCTACATGGCATGCTCCTTGGAAGA-CTATCGG                         GTCATCAGTGGTCACTTGGGATGGGTT
genomic: CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTT
EST: gi|94478034|gb|EB706992.1|EB706992
EST:     CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGG                         GTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
genomic: CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
EST: gi|148950913|gb|EC896543.2|EC896543
EST:     CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGG                         GTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
genomic: CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
EST: gi|148962621|gb|EE013511.2|EE013511
EST:     CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGG                         GTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG
genomic: CTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 465

ATTTTCTACATCCGCACTGATGGAAAGGATACCTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 240

ATTTTCTACATCCGCACTGATGGAAAGGATACCTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 159

ATTTTCTACATCCGCACTGATGGAAAGGATACCTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 227

ATTTTCTACATCCGCACTGATGGAAAGGATACCTAGTAGATGGCCACGGCCTACATGGCATGCTCCTTGGAAGAACTATCGGgtactttctc ... aactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgaattaagagacagttttgattttattccacaaattatgtattgaactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG
               ttttgat  putative branch site (score: 227)
             agttttgattttatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttaagtacaatagtagatttgtttatggtagcattctggttctcatgaattaagagacagttttgattttattccacaaattatgtattgaactacgcagGTCATCAGTGGTCACTTGGGATGGGTTCGATCTATTGCATTTGATCCAGCGAATGAATGGTTCTGCACTGGTTCTGCCGATAGGACAATAAAG

- - - - - - - - - - - - - - - -cattctg
- - - - - - - - - - - - - - - - - - - - - -catgaat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tatgtat