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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagtgatttttagtgtttcatttaagatcttaactaaatgactagttttttcttcttctcccttttccttcacattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC

Basic information

species Zea mays
transcript GRMZM5G869572_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G869572_T01 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os04g59494.1 (Oryza sativa), 3'ss of exon 24
gtgagtg-atttttagtg--tttcatttaagatcttaactaaatgactagttttttcttcttctcccttttccttcacat----tggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC
|| | || ||||| |||   | ||||      | |||    | |||  | ||        | |  | | |  | | ||||    |  ||||| | ||| ||||||||| | ||||| ||||| |||||||||||||||||| ||||||| ||||||||||| |||||||| || ||||| ||||||||||
gtaattgcattttaagttgttctcatcacgtcttttactcgagtgagcatttcagctaaatgcctcgtgtgtcctgacatactattacagGGCTTGAATAAGTCACTTCTCGAAAATAGCGCAATCACTCTTGGGAGGCTTTGCTGGGTCTGTCCAGACATTGTGGCCCCCCACATGGACCATTTCATGC

upper sequence: GRMZM5G869572_T01 (Zea mays), 3'ss of exon 4
lower sequence: Vv18s0001g00910.t01 (Vitis vinifera), 3'ss of exon 24
gtgagtgatttttagtgtttcatttaagatcttaactaaatgactagttttttcttcttctcccttttccttcacatt---ggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC
    || ||  ||  ||||    ||||    |||  | | |  ||| | | ||| |  |     |||    |   |||   |||||||||  || ||||||||||| ||||| || || ||||| ||||||||||||   ||||| || ||||| || || || |  |||||||||||||| ||||
----gt-atcgtttttgttctgcttaatcaatta--ttactttctattatcttcctgattctaatttgagatttgattttgggcagGGTTTTAATAAGTCACTTGTGGAAAATAGTGCCATCACCCTTGGGAGGCTTGCTTGGGTCTGCCCAGAGATTGTAGCACTGCATATGGAGCATTTTATGC

upper sequence: GRMZM5G869572_T01 (Zea mays), 3'ss of exon 4
lower sequence: PP1S276_77V6.1 (Physcomitrella patens), 3'ss of exon 24
---------------------------------------------------gtgagtgatttttagtgtttcatttaagatcttaactaaatgactagttttttcttcttctcccttttcct--tcacattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC
                                                     | || ||  | |  |||   | | |  || | |   | |   || |  || ||  |  |   ||   |     |||   |||||||   |||||||||||  | || || || || || ||  | || || ||  | ||||| |||||||| ||||| || |||||||||||||| |||||||
gtacgtttacccccgtcttcttttcgctttaacacatgtcttttcgctttgaagggtaataataatggttctgtattatttcctgagctattctatattccttgctgttgattgattccacgagctacacgtgcagGGTCTAAACAAGTCACTCTTGGAGAATAGTGCAATTACCTTAGGAAGACTGGGATGGGTTTGTCCAGAGATCGTTGCTCCTCATATGGAGCACTTCATGC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|31363389|gb|CD447746.1|CD447746
EST:     TGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCACAGAG                         GGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTT
genomic: TGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAGgtgagtgatt ... acattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTT
EST: gi|211163359|gb|FK967902.1|FK967902
EST:     TGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAG                         GGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTT
genomic: TGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAGgtgagtgatt ... acattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTT
EST: gi|60338787|gb|DN205760.1|DN205760
EST:     TGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAG                         GGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTT
genomic: TGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAGgtgagtgatt ... acattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 62

ATTGGCAAAGAAATAGCGCCTGTGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAGgtgagtgatt ... acattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 38

ATTGGCAAAGAAATAGCGCCTGTGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAGgtgagtgatt ... acattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 78

ATTGGCAAAGAAATAGCGCCTGTGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAGgtgagtgatt ... acattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC


Block sizes: 44 (upstream exon), 47 (downstream exon)
Score: 31

ATTGGCAAAGAAATAGCGCCTGTGGTGATCACCGTTGTTTCGTGCTTGGTTCCCATTCTAAAATCCCCAGAGgtgagtgatt ... acattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atcttaactaaatgactagttttttcttcttctcccttttccttcacattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC
     aactaaa  putative branch site (score: 31)
               ctagttttttcttctt  putative PPT
                tagtttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagtgatttttagtgtttcatttaagatcttaactaaatgactagttttttcttcttctcccttttccttcacattggcagGGTTCGAACAAGTCACTTGTTGAAAACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGCA